Mohamed A El-Mokhtar1, Khaled M Hassanein1, Ahmed S Ahmed1, Gamal Fm Gad2, Mohamed M Amin3, Osama Fe Hassanein4. 1. Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt. 2. Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia, Egypt. 3. Department of Microbiology and Immunology, Faculty of Medicine, Aswan University, Aswan, Egypt. 4. Drug Research Center, Assiut University, Assiut, Egypt.
Abstract
AIM: This study aimed to describe the inhibitory activity of cell-free supernatants (CFS) of lactobacilli against extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae (K pneumoniae) and Pseudomonas aeruginosa (P aeruginosa). MATERIAL AND METHODS: Pathogenic clinical strains of K pneumoniae and P aeruginosa were isolated from urine samples and selected for investigation. Anti-bacterial activities of the CFS of lactobacilli were assessed by agar well diffusion, MTT assay, as well as time-kill tests. In addition, the antibiofilm characteristics were analyzed by the microplate method against fresh and 24 h-old biofilms. The ability of CFS to interfere with bacterial invasion was analyzed by flow cytometry. RESULTS: Although all tested strains were ESBL producers and showed a multidrug-resistant phenotype, the CFS displayed a high anti-ESBL activity with inhibition zone diameters greater than 13 mm in the agar well diffusion assays against both pathogens. The growth kinetics of K pneumoniae and P aeruginosa were dramatically decreased in the presence of the CFS. The CFS not only inhibited the biofilm formation by these pathogens but also was able to remove the 24-h formed biofilms. The invasion abilities of FITC-labelled K pneumoniae decreased from 30.3%±7 to 15.4%±5 and invasion of FITC-labelled P aeruginosa was reduced from 36.9%±7 to 25.2%±5. CONCLUSION: CFS of lactobacilli exhibit anti-ESBL activities, which suggests its potential application for controlling or preventing colonization of infections caused by ESBL-producing bacteria.
AIM: This study aimed to describe the inhibitory activity of cell-free supernatants (CFS) of lactobacilli against extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae (K pneumoniae) and Pseudomonas aeruginosa (P aeruginosa). MATERIAL AND METHODS: Pathogenic clinical strains of K pneumoniae and P aeruginosa were isolated from urine samples and selected for investigation. Anti-bacterial activities of the CFS of lactobacilli were assessed by agar well diffusion, MTT assay, as well as time-kill tests. In addition, the antibiofilm characteristics were analyzed by the microplate method against fresh and 24 h-old biofilms. The ability of CFS to interfere with bacterial invasion was analyzed by flow cytometry. RESULTS: Although all tested strains were ESBL producers and showed a multidrug-resistant phenotype, the CFS displayed a high anti-ESBL activity with inhibition zone diameters greater than 13 mm in the agar well diffusion assays against both pathogens. The growth kinetics of K pneumoniae and P aeruginosa were dramatically decreased in the presence of the CFS. The CFS not only inhibited the biofilm formation by these pathogens but also was able to remove the 24-h formed biofilms. The invasion abilities of FITC-labelled K pneumoniae decreased from 30.3%±7 to 15.4%±5 and invasion of FITC-labelled P aeruginosa was reduced from 36.9%±7 to 25.2%±5. CONCLUSION: CFS of lactobacilli exhibit anti-ESBL activities, which suggests its potential application for controlling or preventing colonization of infections caused by ESBL-producing bacteria.
Authors: Haidi Karam-Allah Ramadan; Manal A Mahmoud; Mohamed Zakaria Aburahma; Amal A Elkhawaga; Mohamed A El-Mokhtar; Ibrahim M Sayed; Amal Hosni; Sahar M Hassany; Mohammed A Medhat Journal: Infect Drug Resist Date: 2020-10-05 Impact factor: 4.003
Authors: Sawsan Mohammed Kareem; Israa M S Al-Kadmy; Saba S Kazaal; Alaa N Mohammed Ali; Sarah Naji Aziz; Rabab R Makharita; Abdelazeem M Algammal; Salim Al-Rejaie; Tapan Behl; Gaber El-Saber Batiha; Mohamed A El-Mokhtar; Helal F Hetta Journal: Infect Drug Resist Date: 2021-02-12 Impact factor: 4.003
Authors: Olga S Savinova; Olga A Glazunova; Konstantin V Moiseenko; Anna V Begunova; Irina V Rozhkova; Tatyana V Fedorova Journal: Int J Mol Sci Date: 2021-10-12 Impact factor: 5.923