Alan Is Morais1, Xichi Wang2,3,4, Ewerton G Vieira1, Bartolomeu C Viana1,5, Edson C Silva-Filho1, Josy A Osajima1, Samson Afewerki3,4, Marcus Af Corat6, Heurison S Silva5, Fernanda R Marciano5, Guillermo U Ruiz-Esparza3,4, Thiago D Stocco6,7, Mirian Mm de Paula6, Anderson O Lobo1. 1. LIMAV-Interdisciplinary Laboratory for Advanced Materials, Materials Science and Engineering Graduate Program, UFPI-Federal University of Piauí, Teresina, PI CEP 64049-550, Brazil. 2. Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People's Republic of China. 3. Division of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham & Women´s Hospital, Cambridge, MA 02139, USA. 4. Harvard-MIT Division of Health Science and Technology, Massachusetts Institute of Technology, MIT, Cambridge, MA 02139, USA. 5. Department of Physics, UFPI-Federal University of Piauí, Teresina, PI CEP 64049-550, Brazil. 6. Multidisciplinary Center for Biological Research, University of Campinas (UNICAMP), Campinas 13083-877, Brazil. 7. Faculty of Physiotherapy, Santo Amaro University, São Paulo 04829-300, Brazil.
Abstract
BACKGROUND: The facile preparation of oxygen-generating microparticles (M) consisting of Polycaprolactone (PCL), Pluronic F-127, and calcium peroxide (CPO) (PCL-F-CPO-M) fabricated through an electrospraying process is disclosed. The biological study confirmed the positive impact from the oxygen-generating microparticles on the cell growth with high viability. The presented technology could work as a prominent tool for various tissue engineering and biomedical applications. METHODS: The oxygen-generated microparticles fabricated through electrospraying processes were thoroughly characterization through various methods such as X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) analysis, and scanning electron microscopy (SEM)/SEM-Energy Dispersive Spectroscopy (EDS) analysis. RESULTS: The analyses confirmed the presence of the various components and the porous structure of the microparticles. Spherical shape with spongy characteristic microparticles were obtained with negative charge surface (ζ = -16.9) and a size of 17.00 ± 0.34 μm. Furthermore, the biological study performed on rat chondrocytes demonstrated good cell viability and the positive impact of increasing the amount of CPO in the PCL-F-CPO-M. CONCLUSION: This technological platform could work as an important tool for tissue engineering due to the ability of the microparticles to release oxygen in a sustained manner for up to 7 days with high cell viability.
BACKGROUND: The facile preparation of oxygen-generating microparticles (M) consisting of Polycaprolactone (PCL), Pluronic F-127, and calcium peroxide (CPO) (PCL-F-CPO-M) fabricated through an electrospraying process is disclosed. The biological study confirmed the positive impact from the oxygen-generating microparticles on the cell growth with high viability. The presented technology could work as a prominent tool for various tissue engineering and biomedical applications. METHODS: The oxygen-generated microparticles fabricated through electrospraying processes were thoroughly characterization through various methods such as X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) analysis, and scanning electron microscopy (SEM)/SEM-Energy Dispersive Spectroscopy (EDS) analysis. RESULTS: The analyses confirmed the presence of the various components and the porous structure of the microparticles. Spherical shape with spongy characteristic microparticles were obtained with negative charge surface (ζ = -16.9) and a size of 17.00 ± 0.34 μm. Furthermore, the biological study performed on rat chondrocytes demonstrated good cell viability and the positive impact of increasing the amount of CPO in the PCL-F-CPO-M. CONCLUSION: This technological platform could work as an important tool for tissue engineering due to the ability of the microparticles to release oxygen in a sustained manner for up to 7 days with high cell viability.
Authors: Mazaher Gholipourmalekabadi; Susan Zhao; Benjamin S Harrison; Masoud Mozafari; Alexander M Seifalian Journal: Trends Biotechnol Date: 2016-06-17 Impact factor: 19.536
Authors: Shabir Hassan; Berivan Cecen; Ramon Peña-Garcia; Fernanda Roberta Marciano; Amir K Miri; Ali Fattahi; Christina Karavasili; Shikha Sebastian; Hamza Zaidi; Anderson Oliveira Lobo Journal: J Funct Biomater Date: 2021-05-02