Effect of enzymolysis time on biohydrogen production from photo-fermentation by using various energy grasses as substrates.

Energy grass is an ideal raw material of biomass energy, and hydrogen energy is one of the ideal renewable energy. In order to study the feasibility of different energy grasses for hydrogen production from photosynthetic organisms, the enzymatic hydrolysis of energy grasses is a very necessary process. Therefore, biohydrogen production from photo-fermentation by using energy grasses as substrates was investgated by changing enzymolysis time. The hydrogen production results were evaluated by the experimental results of hydrogen yield, hydrogen production rate and hydrogen production efficiency. The experimental results showed that Medicago sativa L. with enzymolysis time of 60 h had the highest hydrogen yield, which was 147.64 mL. The highest hydrogen production rate was 5.53 mL/(h·g TS), which was obtained from Arundo donax with enzymolysis time of 36 h, and the highest hydrogen production efficiency was 1.15 mL/(h·g TS), which was obtained from Miscanthus with enzymolysis time of 0 h.