| Literature DB >> 32109414 |
Shuai Ma1, Shuhui Sun2, Lingling Geng3, Moshi Song4, Wei Wang5, Yanxia Ye6, Qianzhao Ji5, Zhiran Zou5, Si Wang7, Xiaojuan He8, Wei Li8, Concepcion Rodriguez Esteban9, Xiao Long10, Guoji Guo11, Piu Chan8, Qi Zhou12, Juan Carlos Izpisua Belmonte13, Weiqi Zhang14, Jing Qu15, Guang-Hui Liu16.
Abstract
Aging causes a functional decline in tissues throughout the body that may be delayed by caloric restriction (CR). However, the cellular profiles and signatures of aging, as well as those ameliorated by CR, remain unclear. Here, we built comprehensive single-cell and single-nucleus transcriptomic atlases across various rat tissues undergoing aging and CR. CR attenuated aging-related changes in cell type composition, gene expression, and core transcriptional regulatory networks. Immune cells were increased during aging, and CR favorably reversed the aging-disturbed immune ecosystem. Computational prediction revealed that the abnormal cell-cell communication patterns observed during aging, including the excessive proinflammatory ligand-receptor interplay, were reversed by CR. Our work provides multi-tissue single-cell transcriptional landscapes associated with aging and CR in a mammal, enhances our understanding of the robustness of CR as a geroprotective intervention, and uncovers how metabolic intervention can act upon the immune system to modify the process of aging.Entities:
Keywords: aging; caloric restriction; immune cell; inflammation; single-cell RNA atlas; single-cell RNA sequencing; single-nucleus RNA sequencing
Year: 2020 PMID： 32109414 DOI： 10.1016/j.cell.2020.02.008
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582