Comparison of common interventions for the treatment of infantile colic: a systematic review of reviews and guidelines.

OBJECTIVE: To conduct a systematic review of systematic reviews and national guidelines to assess the effectiveness of four treatment approaches (manual therapy, probiotics, proton pump inhibitors and simethicone) on colic symptoms including infant crying time, sleep distress and adverse events.
METHODS: We searched PubMed, Embase, Cochrane and Mantis for studies published between 2009 and 2019. Inclusion criteria were systematic reviews and guidelines that used evidence and expert panel opinion. Three reviewers independently selected articles by title, abstract and full paper review. Data were extracted by one reviewer and checked by a second. Selected studies were assessed for quality using modified standardised checklists by two authors. Meta-analysed data for our outcomes of interest were extracted and narrative conclusions were assessed.
RESULTS: Thirty-two studies were selected. High-level evidence showed that probiotics were most effective for reducing crying time in breastfed infants (range -25 min to -65 min over 24 hours). Manual therapies had moderate to low-quality evidence showing reduced crying time (range -33 min to -76 min per 24 hours). Simethicone had moderate to low evidence showing no benefit or negative effect. One meta-analysis did not support the use of proton pump inhibitors for reducing crying time and fussing. Three national guidelines unanimously recommended the use of education, parental reassurance, advice and guidance and clinical evaluation of mother and baby. Consensus on other advice and treatments did not exist.
CONCLUSIONS: The strongest evidence for the treatment of colic was probiotics for breastfed infants, followed by weaker but favourable evidence for manual therapy indicated by crying time. Both forms of treatment carried a low risk of serious adverse events. The guidance reviewed did not reflect these findings. PROSPERO REGISTRATION NUMBER: CRD42019139074. © Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This study compares meta-data from different treatments for infantile colic on common outcomes.

Guidance and evidence are compared.

Distinguishing superiority of treatments is difficult with multiple subpopulations and outcomes.

Where aetiology of a condition is uncertain, rationale for treatments are difficult to justify.

Effectiveness studies and efficacy studies are needed in this field.

Introduction

Infantile colic, which is defined as excessive crying in the first few months of life, is a common but poorly understood and often frustrating problem for parents and carers. Infantile colic affects somewhere between 3% and 40% of infants worldwide, depending on geography and definitions used.1 It is estimated that around one in six families (17%) with children consult a health professional about symptoms associated with infantile colic, and these include excessive crying, fussing and distress.2

Infants cry for various reasons to express discomfort caused by conditions ranging from benign disorders to life-threatening illness. A meta-analysis published in 2017, which included 28 diary studies covering 8690 infants, reported a mean daily fuss and cry duration of 117 min to 133 min in the first 6 weeks of life, followed by a decline in crying time to a mean of 68 min per day by 10 to 12 weeks of age.3 It is suggested that less than 5% of distressed infants have identifiable medical explanations for their crying.4

There is confusion around the terminology and diagnosis of infantile colic with other diagnostic terms such as silent reflux, functional gastrointestinal disorder and sometimes infantile headache to explain the symptoms of colic. A systematic review of definitions and outcome measures in trials of infantile colic reported the current variability in defining infantile colic, which parallels the non-uniformity of measuring the condition.5 Most definitions are based on Wessel’s criteria, also known as the ‘rule of threes’, which defines colic as paroxysms of irritability, fussing or crying lasting ≥3 hours per day on ≥3 days per week for >3 weeks6 in an otherwise healthy baby aged 2 weeks to 4 months. However, these criteria have been found to be out of date and impractical to use.7 The most recent diagnostic criteria, formulated by the Rome IV committee, are recurrent and prolonged periods of crying without an obvious cause or evidence of failure to thrive or illness in infants younger than 5 months.8

The natural history of infantile colic is favourable with symptoms gradually disappearing by the time the infant is 4 months old.9 However, the impact of excessive infant crying on healthcare services is the most common reason for paediatric consultations and hospital emergency department visits in the first weeks of life.10 The consequences of having an excessively crying infant in the family are harmful to relationships and health.11 Excessive infant crying is associated with maternal issues such as depression, anxiety and loss of parenting confidence.12 13 It is also a common cause of early breastfeeding cessation14 and has been associated with severe infant injury or death as a result of abuse.15

Recommended management strategies usually centre around parental support and reassurance that the infant is otherwise healthy. However, parents are often in a state of crisis and feel that they want to take action. A number of treatment options exist, which include pharmacological treatments (eg, dicyclomine hydrochloride, cimetropium bromide, simethicone and proton pump inhibitors), probiotics, complementary therapies (including herbal agents and sucrose), manual therapies (for example chiropractic, osteopathy and physiotherapy), dietary interventions and parental behavioural interventions.

Reviews to date have assessed the effectiveness of manipulative therapies,16 probiotics,17 dietary modification,18 complementary and alternative therapies (herbal formulations, sucrose or glucose)19 and pain-relieving agents.19 However, there is limited research which compares these treatments to inform the management of infants experiencing colic and parental decision-making.9

Aim

The aim of this study was to review and compare the effectiveness of manual therapy to three of the most common interventions (probiotics, simethicone and proton pump inhibitors) on colic symptoms in infants, including crying time, sleep and infant distress and adverse events.

Methods

We conducted a pragmatic narrative systematic review of systematic reviews (SRs) and clinical guidelines for the most common treatments of infantile colic. We employed a review approach using existing analyses of information whether it was based on compiled narrative analyses, meta-analysis or guideline consensus review. A network meta-analyses for comparing outcomes across treatments was not considered as we were aware from the pre-scoping stage of this review that the studies were too heterogeneous in terms of quality between treatment type. The review protocol was registered on the International prospective register of systematic reviews, and this report follows the guidance in the PRISMA statement20 for reporting reviews.

Eligibility criteria

Our population of interest were infants (under 6 months old) with colic as defined by either Wessel’s or the ROME III or IV criteria.6 7 We were not considering infants with diagnosed gastro-oesophageal reflux disease (GORD), as this is considered a pathological condition and differs from colic in that the infants are generally not thriving.

Only children who were otherwise healthy and thriving were included in this study, that is, no other dominant or serious comorbidity requiring medical care in a non-primary care setting.

We considered four treatments for infantile colic: manual therapy, simethicone, proton pump inhibitors and probiotics as these were considered most widely recognised and of interest to the funder. We included the use of proton pump inhibitors because, while it is not recommended for use in infants with colic, it is increasingly being used with these infants to ‘rule out’ reflux and or GORD, as symptoms of colic are thought to be secondary to GORD.21 Dicyclomine treatment for infantile colic (anti-spasmodic drug) was excluded in this study as it is not recommended for infants under 6 months due to contraindications, mainly respiratory symptoms varying in severity and consequence.22

We defined manual therapy as any predominantly (more than 75%) touch-based therapy administered by a trained and registered manual therapist, such as a chiropractor, osteopath, osteopathic physician, physical therapist or physiotherapist.

The outcomes of interest were crying time, sleep and parental distress, and in addition, where data existed, adverse events data for each treatment were reviewed.

We limited the type of literature we reviewed to systematic reviews of effectiveness and national clinical guidelines.

We included reviews that reported a systematic review methodology and which had more than one researcher indicated in the review process. Narrative literature reviews and editorials were not included.

We included clinical guidelines where clear methodological procedures for development were reported and included systematically designed evidence reviews and expert panel consensus. The guidance had to be intended for broad use at a national level rather than intended as guidance for a single clinic or hospital or a specific setting. We excluded guidance targeted at parents.

We limited the literature to that published in the last decade between 2009 and 2019 and written in English.

Information sources

For systematic reviews, we searched PubMed, MANTIS, EMBASE, Cochrane and for guidelines we searched those published in English by national organisations. The searches were conducted in June 2019.

Search

Key search terms used were ‘systematic reviews’, infant*, colic, ‘manual therapy’, ‘manipulative medicine’, simethicone, ‘proton pump inhibitors’, ‘PPIs’, probiotics, ‘probiotic agents’ (see online supplementary appendix 1).

We searched the central clearing guideline database and known national guideline centres for guidelines on the treatment, management and care of babies with unsettled or distressed behaviour, including infantile colic.

Study selection

Results from searches on each database were downloaded into a Reference Management Software, EndNote (V.X4.0.2), and duplicates were removed; titles and abstracts were screened by two independent researchers. Inclusion/exclusion decisions were made by all three authors. Citation tracking was used to triangulate our searches and check for missing reviews and guidance, as well as to identify other articles that may have not been indexed in PubMed. Full-text papers were obtained for those that met the inclusion criteria and for those where it was unclear whether or not the abstract and title met the inclusion criteria. If a more recent or updated versions of reviews or guidelines were found, the more recent ones replaced those initially found.

Quality appraisal

We appraised the quality of the systematic reviews using a modified version of the AMSTAR 2 critical appraisal tool.23 Of the 15 quality categories assessed, 8 were selected by the authors for the final calculation of quality and we allocated a score of 1 for Yes, 0 for No and 0.5 for Partial Yes. The scale ranged from 0 to 8 (Highest quality). Each study was appraised by two independent reviewers and a third reviewer was used if mitigation was required (see online supplementary appendix 2).

We reported on the quality of the guidelines using a modified version of the AGREE II framework.24 The AGREE II checklist has 23 items evaluating six domains. Where more than 75% of the 23 items (>17/23) were met, we rated these as high-quality guidance. In guidance, where there was insufficient information to record a verdict, we left these domains blank. Guidelines of 16 or less domains did not receive an evaluation of high quality. Each guideline was appraised by one reviewer (see online supplementary appendix 3).

Data extraction and items

We summarised studies by type of intervention, number of studies included, number of participants and outcomes of interest and measure used (table 1). We extracted meta-analysed data on hours of crying time and sleeping time between intervention and control groups and other active interventions. For parent distress and confidence, we proposed to compare effect sizes and/or change scores on similar outcomes. In the absence of meta-analysis, change scores or effect size data, we conducted a narrative synthesis of outcomes.

Table 1

Characteristics of studies for manual therapy for infantile colic

Authors	Participants/n/age/gender	Timing of intervention	Number of studies: type of studies included in review	Method of data synthesis (narrative/meta-analysis)
Alcantara et al 38	Infants/n=980 (where reported)/<18 years/gender not reported	<4 weeks/30 days	26: 3 clinical trials, 2 survey studies, 6 case reports, 2 case series, 4 cohort studies, 5 commentaries and 4 reviews of the literature	Narrative
Carnes et al 43	Infants/n=866 (one study not reported)/gender not reported	8 days to 4 weeks FU 2–11 years	11: 5× RCTs, 1× retrospective review, 2× cohort studies, 3× cases series	Both
Clar et al 39	Infants/n=not reported	Not reported	19: 10 SRs and 9 RCTs	Narrative
Dobson et al 16	Infants/n=358/birth–12 weeks/gender percentages varied (where reported) between 34% and 93% male (mean 56%, median 47%)	8 days to 6 weeks	6 RCTs	Meta-analysis
Driehuis et al 42	Infants/n=283/birth–10 weeks/gender not reported	8–14 days	4 RCTs	Both
Ernst41	Infants/n=198/2–10 weeks/gender not specified	8–15 days	3 RCTs	Narrative
Gleberzon et al 36	Infants/n=1459/birth–3 years	Immediate—4 weeks	6: 3× RCTs, 1× prospective uncontrolled, 2× retrospective studies	Narrative
Parnell Prevost et al 40	Infants/n=>4000/0–12 weeks	Not reported	5: 4 RCTs, 1 obs	Narrative
Posadzki and Ernst27	Infants/n=not reported	Not reported	2 SRs	Narrative
Posadzki et al 37	Infants/n=28/1–12 weeks	28 days	1 RCT	Narrative
Salehi et al 28	Infants/n=368/age=not reported	Not reported	11 SRs—2 SRs for colic	Narrative

FU, follow-up; RCT, randomised controlled trial; SR, systematic review.

We also extracted adverse event incident data and compared risk ratios between treatments and controls where possible. One researcher extracted characteristics and data from the selected articles, and this was checked by a second researcher. A third reviewer’s opinion was sought in cases of disagreement in the extraction process.

Level of evidence

We used reported levels of evidence as published in the reviews and guidance and analysed these to indicate overall level of publication consensus on effectiveness and safety. The strength of the overall evidence was determined by the reviews and guidance evaluated as Favourablesignificant benefit to treatment compared with control; Not favourable—no significant benefit to treatment compared with control or no difference between treatment and controls; Unfavourable—results for treatment worse than control; Inconclusive—unable to draw conclusions from results.

Patient and public involvement

No patient involvement.

Results

We found 201 studies for screening against title and abstract of which 126 were excluded and 75 were selected for full paper review. Of these, a further 43 were excluded and 32 references were finally included in this systematic review. See figure 1 flow chart illustrating the process and reasons for exclusion.

Figure 1

Flowchart of search process for the review. PPI, proton pump inhibitor.

Eleven studies investigated the effectiveness of manual therapy for treating infantile colic, 12 for probiotics, 1 for proton pump inhibitors, 4 for simethicone and 4 papers which included a combination of the interventions of interest in this review.

The characteristics of the final selection of studies are shown in tables 1–5 ordered by interventions: manual therapy, simethicone, probiotics, proton pump inhibitors and mixed interventions.

The control arms varied for the studies included in the systematic reviews: for example, in the probiotic, proton pump inhibitors and simethicone studies, the controls were mainly placebos or usual feeding method (mainly breast feeding); and in the manual therapy studies, the controls were either no treatment, usual care, dimethicone and in one craniosacral decompression.

Outcome measures

Crying time was the most reported outcome (26/32 studies). In three studies, crying episodes (as opposed to crying duration) were recorded as the outcome measure.18 25 26 Three studies did not report outcome data.27–29

Fourteen of the 26 papers that reported crying time conducted a meta-analysis. One study30 reported outcomes for all three interventions and another31 reported meta-analysed data for probiotic use and narrative analysis for simethicone. There were 10 meta-analysed results for probiotics, 2 for simethicone and 4 for manual therapy. The data indicated that for breastfed infants, probiotics significantly decreased daily crying time (range −25 min to −65 min). The level of evidence for these findings was reported as high (as indicated by the systematic reviewer authors’ appraisal of quality and/or risk of bias) with seven studies reporting high quality, one moderate and two low quality. There were four systematic reviews investigating effectiveness of manual therapy for crying, three showed favourable effects (from −33 min to −76 hours of crying time reduction in 24 hours) and one review was inconclusive. The quality of these randomised controlled trials (RCTs) ranged from moderate to low by the authors of the SRs.

The findings from two studies investigating the effects of simethicone were inconclusive or not favourable and were graded as moderate/low quality. Table 6 shows the meta-analysed data for crying time.

Table 6

Summary of meta-analyses of treatments for infant colic measured by crying time

Author	Time point	24 hours crying time	Effect	Level of evidence	AMSTAR
Probiotics*
Ong et al 17	30–90 days	−32.57 min(95% CI −55.6 to −9.54)	Favourable	Low	7
Dryl and Szajewska54	21 days and 28 days	−49 min(95% CI −66 to −33) for L.reuteri only	Favourable	High	5.5
Sung et al 57	21 days	−25.4 min (95% CI −47.3 to −3.5)	Favourable	High	6.5
Gutiérrez-Castrellón et al 30	7–28 days	WMD −51.3 min (95% CI −30.5 to −72.2 min), p=0.0001	Favourable	High	7
Schreck Bird et al 55	21 days28 days	2.3 times more likely to have a ≥50% reduction (p=0.01)	Favourable	High	6
Harb et al 31	21 and 30 days	−55.8 min(95% CI −64.4 to −47.3)	Favourable	High	8
Xu et al 58	14 days21 days	−42.89 min(95% CI –60.50 to –25.29; p=0.000)−45.83 min(95% CI –59.45 to –32.21; p=0.000)	Favourable	High	7
Urbańska and Szajewska59	21 days1 month	−43 min/day(95% CI −68 to −19)	Favourable	High	4.5
Sung et al 56	21 days	−65 min(95% CI −86 to −44)	Inconclusive	Low	8
Anabrees et al 52	21 and 28 days	−56.03 min (95% CI −59.92 to −52.15), RR of 0.06 (95% CI 0.01 to 0.25) NNT 2	Favourable	Moderate	8
Simethicone
Gutiérrez-Castrellón et al 30	7–28 days	WMD −30.0 min (95% CI −20.8 to −39.0 min), p=0.001 for drugs including simethicone	Inconclusive	Moderate	7
Biagioli et al 19	7 days	−0.13 hours(95% CI −1.40 to 1.14)	Not favourable	Low	8
Manual therapy
Carnes et al 43	1–4 weeks	−1.27 hours(95% CI −2.19 to −0.36)	Favourable	Moderate	8
Gutiérrez-Castrellón et al 30	7–28 days	WMD −37.4 min (95% CI −21.5 to −67.0 min), p=0.001	Inconclusive	Moderate	7
Dobson et al 16	8 days to 4 weeks	−1.2 hours(95% CI −1.89 to 0.51)	Favourable	Low	8
Driehuis et al 42	8–10 days	−0.33 hours per day (95% CI −0.012 to 0.59)	Favourable	Low	8

*Predominantly breastfed infants.

NNT, number needed to treat; RR, risk ratio; WMD, weighted mean difference.

There were three studies that reported the number of crying episodes; one was a meta-analysis on the effectiveness of probiotics25 and the other two were narrative analyses, one for probiotics26 and one where simethicone was used as the control arm compared with partially hydrolysed formula.18 The results from these three studies did not support the use of probiotics or simethicone for the treatment of infantile colic and the quality of the studies were reported as low in all studies (see table 7).

Table 7

Summary of meta-analyses of treatments for infant colic measured by number of crying episodes

Author	Intervention	Time point	Number of crying episodes	Effect	Level of evidence	AMSTAR
Gordon et al 18	Simethicone	14 days	3.32 more episodes of crying and fussing with simethicone compared with partially hydrolysed formula	Not favourable c/w partially hydrolysed formula	Very low	8
Mugambi et al 25	Probiotics	Up to 7 months	MD 0.60 (95% CI 0.20 to 1.00) in favour of control for 1 out of 4 studies reported	Not favourable	Low	8
Skórka et al 26	Probiotics	4 weeks to 36 months	Colic symptoms and crying	Inconclusive (not favourable)	Low	7

MD, mean difference.

Narrative analysis

Crying time

Thirteen of the 26 studies reporting on crying times conducted a narrative review. Two of the studies2 32 reported on the effectiveness of both probiotics and manual therapy for treating infantile colic. Of the four studies that reported on probiotics, one reported a favourable outcome when compared with simethicone.33 The other studies did not support the use of probiotics and were of low-grade quality.

Two reviews narratively reported on the effects on crying time using simethicone; the findings did not support its use.31 34 The quality of the studies in these reviews were graded as low by the review authors.

One review reported on crying time, crying episodes, crying time post feeding and parent perception of change in fussing time between infants taking proton pump inhibitors and a placebo. They found no significant differences between groups on any outcome and concluded in the light of associated adverse events unfavourable evidence for the use of proton pump inhibitors.35

Eight studies reported on the effectiveness of manual therapy for infantile colic. Two studies reported a favourable outcome,36 37 four were inconclusive,32 38–40 one study showed no beneficial effects over the control arm41 and one study reported an unfavourable outcome.2 Most of the reviews for manual therapy graded the RCT studies as low quality.

The findings are summarised in table 8.

Table 8

Summary of narrative analyses of treatments for infant colic measured by crying time

Author	vs control	Time point	Effect	Level of evidence	AMSTAR
Probiotics
Batchelor et al 53	Not reported	21 days and 90 days	Not favourable	Not reported	2.5
Cruchet et al 33	Simethicone	7, 14 and 21 days	Favourable	High prevention, Moderate treatment	4.5
Lucassen2	Placebo	21 days	Inconclusive	Very Low	2
Perry et al 32	Placebo andSimethicone	14 and 28 days	Inconclusive	Low	8
Simethicone
Harb et al 31	Placebo, mint and probiotics	17–21 days	Not favourable	Not reported	8
Hall et al 34	Placebo	2–8 weeks	Not favourable	Low	4
Manual therapy
Alcantara et al 38	Dimethicone, no treatment, occipitosacral decompression	14 days and 4 weeks where reported	Inconclusive	Not reported	5
Ernst41	Not reported	8 days to 2 weeks	Not favourable	Low	4.5
Lucassen2	No treatment	10–14 days	Unfavourable	Low	2
Perry et al 32	Dimethicone or no treatment	8 days to 4 weeks	Inconclusive	Low	8
Clar et al 39	Not reported	Not reported	Inconclusive (favourable)	Low tomoderate	8
Gleberzon et al 36	Not reported	<4 weeks	Favourable	Low	6
Parnell Prevost et al 40	Not reported	Not reported	Inconclusive	Moderate to high	7
Posadzki et al 37	Not reported	4 weeks	Favourable(1 RCT only)	Low	7
Proton pump inhibitors
Gieruszczak-Białek et al 35	Placebo	2–4 weeks	Not favourable	Not reported	3.5

RCT, randomised controlled trial.

Three further narrative studies, two for manual therapy27 28 and one for simethicone,29 did not report details on how the effectiveness of interventions was measured. The results of these studies were reported as unfavourable or inconclusive and the quality of the studies were low.

Adverse events

When reported, there were no serious adverse events reported in the RCTs for either manual therapy, simethicone or probiotics. In one SR,42 four serious adverse events were documented in case studies of manual therapy, including a death, temporary paralysis and a rib fracture, but because these were poorly described and missed underlying pathology could not be ruled out, the overall risk where reported was low. Risks of developing infections with probiotics were reported as extremely low.33 The review on PPIs showed both arms had adverse events, one RCT showed a significant difference between lansoprazole and the placebo for totality of serious adverse effects, mainly lower respiratory tract infections (10/81 vs 2/81, risk ratio 5, 95% CI 1.3 to 20, number needed to harm 11, 95% CI 6 to 49)21 (see online supplementary appendix 4).

Guidelines for the treatment, care and management of infants with colic

We found three national clinical guidelines about the treatment and management of ‘colicky’ infants.

Table 9 summarises the recommendations they made. The quality rating for the UK guideline development process and reporting met 18/23 criteria, the USA and the Irish guidance 11/23.

Table 9

National guideline recommendations infantile colic

Recommendation/suggestion	UK 2013 2017*	USA 2015†	Ireland 2014‡
Clinician evaluation of mother and baby	✓	✓	✓
Parenting information, advice, support and reassurance	✓	✓	✓
Continue breast feeding	✓	✓
Maternal diet modification	✕	✓
Change formula if formula fed (+unless milk allergy identified)	✕+	✓
Probiotic supplements (++breastfed-only infants)	✕	✓++	✓
Simethicone (eg, infacol)	✕	✕
Herbal supplements (eg, fennel)	✕	✕
Proton pump inhibitors (eg, omeprazole, Losec)		✕
Lactase (eg, Co-lief drops)	✕
Anitcholinergic medication (including dicyclomine)		✕
Gripe water		✕
Medicine generally
Infant massage			✓
Manual therapy (including spinal manipulation and cranial osteopathy)	✕	✕
Physical contact (eg, holding, rocking)	✓		✓
White noise	✓
Bathing	✓
Winding	✓
Swaddling		✕
Acupuncture		✕
Sleep routine

Blank boxes indicate no recommendation or suggestion made not considered or reported. Ticks indicate recommended interventions; cross indicates non-recommended interventions.

*UK NICE www.nice.org.uk Clinical Knowledge Summary Infant Colic 2017 and Postnatal care: routine postnatal care of women and their babies (NICE Guideline, 2015).

†USA 2015 American Academy of Family Physicians (http://www.aafp.org).

‡Ireland: Irish College of General Practitioners (https://www.icgp.ie).

Discussion

Summary of findings and context

We found 32 relevant systematic reviews and three examples of guidance. Many of the RCTs were repeated within the reviews and this is reflected by fairly consistent results but differing interpretations. Overall, the meta-analysed results showed that both probiotics in breastfed infants and manual therapy can reduce crying time. The daily reduction in crying is between 33 and 76 min with manual therapy and between 25 min and 65 min with probiotics in breastfed infants. The quality and strength of evidence was higher for probiotics than manual therapy. The evidence for probiotics centred on breastfed infants rather than formula-fed infants and there were a number of different types of strains of probiotics. The manual therapy evidence was based on low to moderate quality RCTs and therefore larger blinded RCTs were recommended. In addition, crying time was reported as the primary outcome in most studies which was used as a proxy indicator of colic resolution or improvement.

There were no serious adverse events reported for either probiotics or manual therapy, indicating that both represent a low risk to infants, although we cannot conclude they are without any risk.27 42 Two reviews16 43 analysed the risks of adverse events with manual therapy, one showed 88% less risk of an adverse event in the manual therapy groups than in the control groups43 and the other showed one in six parents reported non-serious adverse events.16 Another study42 reported data from non-RCTs which included four case studies reporting serious incidents of harm but there was some doubt over causality as a result of the treatments given. The risk with probiotic was very low.17

The data for simethicone and proton pump inhibitors were unfavourable with five reviews concluding either no difference or worsening of symptoms with the use of simethicone. One review concluded no significant differences in crying time or episodes with proton pump inhibitors compared with a placebo, but there was evidence of serious adverse events with the proton pump inhibitor group (one RCT).42 Other older reviews have concluded the same.22

We found few systematic reviews assessing the effectiveness of PPIs for colic despite their increasing use in this population.21 44 This is because most studies investigating the use of PPIs were for GORD with symptoms of colic. We are aware, however, of the practice of using PPIs to diagnose GORD by treatment.44 PPIs are designed to suppress acid but have consistently been shown to be ineffective for irritability and fussing in infants with GORD, and they are associated with an increased risk of adverse effects such as infections, allergies and hospital admissions.9 21 35 45 46 There is evidence from Australia to show that PPIs are being over-prescribed for infants with physiological reflux and symptoms of colic who may or do not have GORD. The reasons are complex, but the authors suggest they centre around inconsistent diagnostic criteria and diagnostic labelling and ‘defensive’ medicine practice to substantiate the diagnosis with the expectation to medicate.47 None of the guidance reviewed recommended the use of PPIs for the treatment of colic.

We found three nationally representative guidance, and the only other guidance we found was directed at parents with unclear sources of evidence and guidance justification. Clinical evaluation, information, advice, support and reassurance were the only guidance that was agreed in all four guidelines. Three of the four guidelines recommended to continue to breast feed and use physical contact and not to recommend simethicone and manual therapy, despite the difference in current evidence between them, that is, favourable moderate to low quality for manual therapy and unfavourable low quality for simethicone. Despite the stronger evidence for probiotics in breastfed infants, this was only recommended as a treatment to consider in the USA and Irish guidance. The Canadian Paediatric Association issued a position statement in 2012 which was updated in 2019 stating: “While there may be a role for probiotics in treating infantile colic, there is insufficient evidence to recommend for or against using probiotics to manage this condition”.48

Only the USA guidance specifically states not to use proton pump inhibitors for the treatment of colic. We do not know the level of proton pump inhibitors use in the infantile colic population; further research is needed to understand the prescribing practice in this field and the impact on the infants. Conversely, UK NICE guidance development group for the diagnosis and management of GORD stated that: “it was not unreasonable to offer a trial of either an H).

Interestingly, the guidance and the evidence do not reflect each other; this may be due to the timing of published evidence and guideline development. The lack of consistent guidance available for parents, pharmacists and clinicians compounds the uncertainty relating to the care of infants with the symptoms of colic.49

We did not find any evidence around cost-effectiveness analyses of the treatments and there was not enough data to analyse our other outcomes of interest, sleep and parental distress.

Strengths, limitations and challenges

This review was limited to assessing four treatments. The two treatments, probiotics and manual therapy, indicated an effect on reducing crying time. We do not know, however, whether this reduction is meaningful to parents or whether it is sufficient to improve the parent/infant relationship and/or the well-being of the infants, their parents and siblings. We did not compare manual therapy against other therapies such as dietary modification, herbal remedies, white noise, winding, swaddling and baby massage as suggested in some of the guidance. These other remedies and approaches do not appear to have a strong evidence base but are perhaps deemed to be relatively risk free by the expert consensus guidance panels. Although one recent editorial suggests that herbal mixtures and swaddling, while potentially effective, may be harmful because herbal mixtures may affect optimal milk consumption and swaddling may increase hip dysplasia.9 Simethicone and proton pump inhibitors were not considered as risk free and were not recommended in the guidance either explicitly or implicitly.

In addition, it was not always clear what manual treatments were given and how many; therefore, we are unable to comment on type or quantity of manual treatment that may or may not have an effect.

We reviewed data published in the last decade to avoid over-duplication and we only reviewed articles published in English in peer-reviewed journals; this may have introduced some publication bias.

We suspect that the number and diversity of treatments that exist for infantile colic are borne out of desperation and lack of understanding about the aetiology of the colic and its persistence, although self-limiting in most cases. It has also been suggested that this has led to heterogeneous outcomes being measured,11 but several reviews were able to meta-analyse reduction in crying time. None of the reviews measured the well-being or confidence of the parents in managing their infants, or relationships and bonding between the parents and the infants and/or their siblings.

The strength of this review is that we have compared at least two treatments—probiotics and manual therapy—for colic and compared the recommendations in the guidance with the evidence which highlighted the difference in certainty between the evidence and the recommendations. This review found favourable but low to moderate quality evidence for manual therapy. This is different to other reviews, which generally reject manual therapy as an option due to poor quality data. New studies have raised the quality level of data and our more favourable interpretation may, in part, be due to the background of the authors and their understanding that manual therapy is a multicomponent therapy consisting of more than touch alone. The role of the manual therapist to reassure, guide, advise and support parents through this particularly difficult time may also have a therapeutic role to play which may affect parenting and outcomes. More high-quality studies are needed to increase the level of certainty surrounding the findings about the effect of manual therapy for infantile colic.

Future studies should consider the effect of treatment on the parents to explore parenting confidence, parent/infant bonding and meaningful levels of change in crying and sleeping time.

Further research will probably change recommendations in the guidance as the evidence evolves and increases in quality. The guidance we reviewed did not reflect the emerging evidence. Overall, there is uncertainty about the management and care of infants with infantile colic in part due to the lack of consensus surrounding its aetiology and justification for treatments, although increasingly, newer research is providing plausible mechanistic explanations why probiotics may be beneficial due to the presence of gut inflammatory markers.50 51 Infantile colic is self-limiting in nature and understanding what symptoms and the level of reduction of these symptoms that are important to the infants and their carers needs further investigating. It is understandable that a range of approaches to treatment, management and care exist. Preserving parent choice and balancing this with the limited evidence about effectiveness and safety remains difficult to determine.

Conclusions

We found that the strongest evidence for the treatment of infantile colic was probiotics, particularly Lactobacillus reuteri for breastfed infants, followed by weaker but favourable evidence for manual therapy indicated by crying time. Both forms of treatment carried a low risk of serious adverse events. Current guidelines will probably change over time in light of existing new and emerging evidence.

Table 2

Characteristics of studies for probiotics for infantile colic

Authors	Participants/n/age/gender	Treatment and timing of intervention	Number of studies: type of studies included in review	Method of data synthesis (narrative/meta-analysis)
Anabrees et al 52	Breastfed or partly breastfed infants/n=209/<4 months/gender not reported	L.reuteri (strains—American Type Culture Collection Strain 55730 and DSM 17938)21 days and 28 days	3: 1× Open prospective randomised study, 2× double-blind RCTs	Meta-analysis
Batchelor et al 53	Infants/n=924/newborns <3 months (where reported)/gender not reported	L.reuteri. 21 days and 90 days (where reported)	5: 3× RCTs, 1× observational prospective and 1× meta-analysis	Narrative
Cruchet et al 33	Infants/n=not reported/age not reported/gender not reported	L.reuteri, B.lactis and Str. thermophiles Not reported	9: 1× SR, 8× RCTs	Narrative
Dryl and Szajewska54	Infants/n=471/<6 months/gender not reported	L.reuteri. 21–30 days	7 RCTs	Meta-analysis
Mugambi et al 25	Healthy infants	Mixed: synbiotics, probiotics, prebiotics (various strains used). Not reported	25 RCTs	Meta-analysis
Ong et al 17	Infants/n=1886/<1 month	L.reuteri DSM, multistrain probiotics, L. rhamnosus, L. paracasei and B. animalis 4/52 before delivery to 6 months	6 RCTs	Meta-analysis
Schreck Bird et al 55	Infants/n=444/31–52 days	L.reuteri DSM 17938 (108 CFU)21 and 28 days	5 RCTs	Narrative
Skórka et al 26	Infants/4 weeks to 36 months	Various probiotic strains included. 4 weeks to 12 months	20 RCTs	Narrative
Sung et al 56	Infants/n=1825/<3 months	Various strains probiotics. 2 weeks to 6 months	12 RCTs	Meta-analysis
Sung et al 57	Infants/n=345/birth to 23 months	L.reuteri DSM 1793821 days	4 RCTs	Meta-analysis

CFU, colony-forming unit; RCT, randomised controlled trial; SR, systematic review.

Table 3

Characteristics of studies for simethicone for infantile colic

Authors	Participants/n/age/gender	Timing of intervention	Number and type of studies included in review	Method of data synthesis (narrative/meta-analysis)
Biagioli et al 19	Infants/n=167/<5 weeks/males 45.5%, 44.4%, 49.4%	7 days	4 RCTs	Meta-analysis
Gordon et al 18	Infants/n=1121/2–16 weeks/balanced numbers of boys and girls	4–21 days	1 RCT	Narrative
Hall et al 34	Infants/n=309 (27/32 and 83/92 and 199/267)/2–8 weeks/gender not reported	Not reported	3 RCTs	Narrative
Salvatore et al 29	1 consensus review, 1 review, 1 SR	NA	NA	Narrative
Xu et al 58	Infants/n=423/3–6 months	L.reuteri. 21 and 28 days	6 RCTs	Meta-analysis
Urbańska and Szajewska59	Infants/n=838/birth to 26 weeks	L.reuteri. Up to 1 month for management group and up to 3 months for the prevention group	5 RCTs	Meta-analysis

NA, not available; RCT, randomised controlled trial; SR, systematic review.

Table 4

Characteristics of studies for proton pump inhibitors for infantile colic

Authors	Participants/n/age/gender	Timing of intervention	Number and type of studies included in review
Gieruszczak-Białek et al 35	Infants/n=404/<12 months	2–4 weeks	5 RCTs	Narrative

RCT, randomised controlled trial.

Table 5

Characteristics of studies for mixed intervention studies for infantile colic

Authors	Intervention	Participants/n/age/gender	Timing of intervention	Number and type of studies included in review	Method of data synthesis (narrative/meta-analysis)
Gutiérrez-Castrellón et al 30	Probiotics (L. reuteri)	Infants/n=347/4.1–7.2 weeks/56% female	21 and 28 days	5 RCTs	Meta-analysis
	Manual therapy	Infants/n=257/5.1–5.9 weeks/56% female	11–28 days	5 RCTs	Meta-analysis
	Simethicone	Infants/n=14/4.1 weeks/56% female	17 days	1 RCT	Meta-analysis
Harb et al 31	Probiotics (L. reuteri)	Infants less than 6 months old/n=472	21 and 30 days	7: 5 double-blind RCTs, 1 single blind RCT, 1 prospective RCT	Meta-analysis
	Simethicone	Infants 2 to 8 weeks old/n=193	Not reported	3 RCTs (simethicone was intervention in 1 and control in 2)	Narrative
Lucassen2	Manual therapy	Infants/n=not reported/1–9 weeks/gender not reported	10–14 days	1 SR	Narrative
	Probiotics (L. reuteri)	Infants/n=126/2–16 weeks/gender not reported	Not reported	1 SR	Narrative
Perry et al 32	Probiotics (L. reuteri, L. rhamnosus and P. freudenreichii)	Infants/n=101/0–16 weeks/M=47, F=45	14 days and 28 days	2 RCTs	Narrative
	Manual therapy	Infants/n=not reported /0–16 weeks/gender not reported	Not reported	4 RCTs	Narrative

RCT, randomised controlled trial; SR, systematic review.