Kimie Tanaka1,2, Daiju Fukuda3, Yasutomi Higashikuni2, Yoichiro Hirata4, Issei Komuro2, Toshiki Saotome5, Yoshihiro Yamashita6, Tetsuo Asakura7, Masataka Sata8. 1. Division for Health Service Promotion, the University of Tokyo. 2. Department of Cardiovascular Medicine, the University of Tokyo. 3. Department of Cardio-Diabetes Medicine, Tokushima University Graduate School of Biomedical Sciences. 4. Department of Pediatrics, the University of Tokyo Hospital. 5. Research and Development Center, The Japan Wool Textile Co., Ltd. 6. Research Center for Fibers and Materials, University of Fukui. 7. Department of Biotechnology, Tokyo University of Agriculture & Technology. 8. Department of Cardiovascular Medicine, Tokushima University Graduate School of Biomedical Sciences.
Abstract
AIM: Synthetic vascular grafts are widely used in surgical revascularization, mainly for medium- to large-sized vessels. However, synthetic grafts smaller than 6 mm in diameter are associated with a high incidence of thrombosis. In this study, we evaluated silk fibroin, a major protein of silk, with high biocompatibility and biodegradability, as a useful material for extremely-small-diameter vascular grafts. METHODS: A small-sized (0.9 mm inner diameter) graft was braided from a silk fibroin thread. The right carotid arteries of 8- to 14-week-old male C57BL/6 mice were cut at the midpoint, and fibroin grafts (5- to 7-mm in length) were transplanted using a cuff technique with polyimide cuffs. The grafts were harvested at different time points and analyzed histologically. RESULTS: CD31＋ endothelial cells had already started to proliferate at 2 weeks after implantation. At 4 weeks, neointima had formed with α-smooth muscle actin+ cells, and the luminal surface was covered with CD31＋endothelial cells. Mac3＋ macrophages were accumulated in the grafts. Graft patency was confirmed at up to 6 months after implantation. CONCLUSION: This mouse model of arterial graft implantation enables us to analyze the remodeling process and biocompatibility of extremely-small-diameter vascular grafts. Biodegradable silk fibroin might be applicable for further researches using genetically modified mice.
AIM: Synthetic vascular grafts are widely used in surgical revascularization, mainly for medium- to large-sized vessels. However, synthetic grafts smaller than 6 mm in diameter are associated with a high incidence of thrombosis. In this study, we evaluated silk fibroin, a major protein of silk, with high biocompatibility and biodegradability, as a useful material for extremely-small-diameter vascular grafts. METHODS: A small-sized (0.9 mm inner diameter) graft was braided from a silk fibroin thread. The right carotid arteries of 8- to 14-week-old male C57BL/6 mice were cut at the midpoint, and fibroin grafts (5- to 7-mm in length) were transplanted using a cuff technique with polyimide cuffs. The grafts were harvested at different time points and analyzed histologically. RESULTS: CD31＋ endothelial cells had already started to proliferate at 2 weeks after implantation. At 4 weeks, neointima had formed with α-smooth muscle actin+ cells, and the luminal surface was covered with CD31＋endothelial cells. Mac3＋ macrophages were accumulated in the grafts. Graft patency was confirmed at up to 6 months after implantation. CONCLUSION: This mouse model of arterial graft implantation enables us to analyze the remodeling process and biocompatibility of extremely-small-diameter vascular grafts. Biodegradable silk fibroin might be applicable for further researches using genetically modified mice.
Authors: Gregory H Altman; Frank Diaz; Caroline Jakuba; Tara Calabro; Rebecca L Horan; Jingsong Chen; Helen Lu; John Richmond; David L Kaplan Journal: Biomaterials Date: 2003-02 Impact factor: 12.479