Qing Xiao1, Jiayi Ying1, Zhuhui Qiao1, Yiwen Yang1, Xiaoxi Dai1, Zhongyi Xu1, Chengfeng Zhang2, Leihong Xiang3. 1. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, PR China. 2. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, PR China. Electronic address: e3dangdang@hotmail.com. 3. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, PR China. Electronic address: flora_xiang@vip.163.com.
Abstract
BACKGROUND: Melanoma is one of the most aggressive, therapy-resistant skin cancers in the world. Hydrogen sulfide (H2S), a newly discovered gasotransmitter, plays a crucial role in the progression and development of many types of cancers. However, the effect of H2S on human skin melanoma remains to be elucidated. OBJECTIVE: We aimed to explore the effect of exogenous H2S on melanoma cells and its underlying mechanisms. METHODS: In this study, human skin melanoma cell lines, including A375 and SK-MEL-28, were treated with a donor of H2S (NaHS). CCK-8, scratch assay, flow cytometric analysis, western blotting and transmission electron microscopy (TEM) were performed to explore the effects of H2S on cell behaviors. RESULTS: Treatment with NaHS inhibited cell proliferation, migration and division, while it could induce cell apoptosis and autophagy in melanoma cell lines. Moreover, NaHS significantly decreased the expression of p-PI3K, p-Akt and mTOR proteins. Furthermore, insulin-like growth factor-1 (IGF-1), the activator of PI3K/AKT/mTOR pathway, could reverse the cell behaviors caused by NaHS. CONCLUSION: Our results demonstrated that exogenous hydrogen sulfide could inhibit human melanoma cell development via suppression of the PI3K/AKT/mTOR pathway. Hydrogen sulfide might serve as a potential therapeutic option for melanoma.
BACKGROUND:Melanoma is one of the most aggressive, therapy-resistant skin cancers in the world. Hydrogen sulfide (H2S), a newly discovered gasotransmitter, plays a crucial role in the progression and development of many types of cancers. However, the effect of H2S on humanskin melanoma remains to be elucidated. OBJECTIVE: We aimed to explore the effect of exogenous H2S on melanoma cells and its underlying mechanisms. METHODS: In this study, humanskin melanoma cell lines, including A375 and SK-MEL-28, were treated with a donor of H2S (NaHS). CCK-8, scratch assay, flow cytometric analysis, western blotting and transmission electron microscopy (TEM) were performed to explore the effects of H2S on cell behaviors. RESULTS: Treatment with NaHS inhibited cell proliferation, migration and division, while it could induce cell apoptosis and autophagy in melanoma cell lines. Moreover, NaHS significantly decreased the expression of p-PI3K, p-Akt and mTOR proteins. Furthermore, insulin-like growth factor-1 (IGF-1), the activator of PI3K/AKT/mTOR pathway, could reverse the cell behaviors caused by NaHS. CONCLUSION: Our results demonstrated that exogenous hydrogen sulfide could inhibit humanmelanoma cell development via suppression of the PI3K/AKT/mTOR pathway. Hydrogen sulfide might serve as a potential therapeutic option for melanoma.