X-H Zhang1, Z-M Xin. 1. Department of Burn and Plastic Surgery, Affiliated Success Hospital of Xiamen University (The 174th Hospital of PLA), Xiamen, China. zxyyxzm@163.com.
Abstract
OBJECTIVE: The aim of this study was to investigate the potential effects of microRNA-135b-5p (miR-135b) on the development of malignant melanoma (MM) and the relevant mechanism. PATIENTS AND METHODS: The expression level of miR-135b in MM tissues and cells was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Online prediction software and luciferase reporter assays were used to predict and verify the possible target of miR-135b, respectively. Furthermore, the effects of the miR-135b on MM A375 cells were determined by Western blotting, MTT, and transwell assays. RESULTS: MiR-135b was significantly down-regulated in MM. RING-box protein 1 (RBX1) was verified as a direct target of miR-135b. Subsequent experiments showed that down-regulation of RBX1 resulted from miR-135b up-regulation could significantly inhibit the proliferation, invasion, and migration abilities of MM cells. CONCLUSIONS: MiR-135b inhibited the progression of MM by targeting RBX1. Our findings revealed that miR-135b/RBX1 might be a potential therapeutic target for the treatment of MM.
OBJECTIVE: The aim of this study was to investigate the potential effects of microRNA-135b-5p (miR-135b) on the development of malignant melanoma (MM) and the relevant mechanism. PATIENTS AND METHODS: The expression level of miR-135b in MM tissues and cells was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Online prediction software and luciferase reporter assays were used to predict and verify the possible target of miR-135b, respectively. Furthermore, the effects of the miR-135b on MMA375 cells were determined by Western blotting, MTT, and transwell assays. RESULTS:MiR-135b was significantly down-regulated in MM. RING-box protein 1 (RBX1) was verified as a direct target of miR-135b. Subsequent experiments showed that down-regulation of RBX1 resulted from miR-135b up-regulation could significantly inhibit the proliferation, invasion, and migration abilities of MM cells. CONCLUSIONS:MiR-135b inhibited the progression of MM by targeting RBX1. Our findings revealed that miR-135b/RBX1 might be a potential therapeutic target for the treatment of MM.
Authors: Mireia Olivan; Marta Garcia; Leticia Suárez; Marc Guiu; Laura Gros; Olga Méndez; Marina Rigau; Jaume Reventós; Miguel F Segura; Inés de Torres; Jacques Planas; Xavier de la Cruz; Roger R Gomis; Juan Morote; Ruth Rodríguez-Barrueco; Anna Santamaria Journal: Cancers (Basel) Date: 2021-12-09 Impact factor: 6.639