Functional properties of presumed dopamine-containing and other ventral tegmental area neurons in conscious rats.

To elucidate the functional significance of mesolimbocortical dopamine (DA)-containing neurons in animal adaptive activity, the properties of single units in ventral tegmental area (VTA) and adjacent regions of the midbrain were studied in conscious rats with strictly fixed skull. Analysis of spontaneous firing activity, its changes during polymodal activating and aversive stimulations and their interrelations was performed in electrophysiologically-identified presumed DA-containing (D-type, 48 cells) and other (A-B and C-type, 47 and 29 cells accordingly) neurons found in this brain area. A common feature of all cells was the dependence of their discharge changes on the biological significance of the stimulation used and the strong correlation between these firing changes and stimulation-induced or spontaneous movement activity and blood pressure oscillations. Moreover, a significant correlation between the rate of firing and its dispersion and constancy of directions of neuronal changes during experimental stimulation in single cells were found. Presumed DA-containing neurons of D-type had a high variability of all properties and heterogeneity in the pattern of their discharges and in direction changes (prevalent activations). In presumed acetylcholine (ACh)-containing A-B type cells strong tonic-like activations and in presumed GABA- or ACh-containing interneurons of C-type depressions of firing both correlated with animal movement activity were found. Present data were discussed in relation with mediator specifity of studied cells and the differences of their participation in avoidance behavior forming in aversive environment.