Anne Lundby Hess1, Lesli Hingstrup Larsen1, Pernille Baekgaard Udesen2,3, Yolanda Sanz4, Thomas Meinert Larsen1, Louise Torp Dalgaard2. 1. Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark. 2. Department of Science and Environment, Roskilde University, Roskilde, Denmark. 3. Department of Gynaecology and Obstetrics, Fertility Clinic, Zealand University Hospital, Køge, Denmark. 4. Microbial Ecology, Nutrition and Health Research Unit, Institute of Agrochemistry and Food Technology, National Research Council (IATA-CSIC), Valencia, Spain.
Abstract
OBJECTIVE: This study investigated whether the levels of specific serum microRNAs (miRNAs) were altered following diet-induced weight loss and whether the serum miRNAs differed in the presence of the metabolic syndrome. METHODS: The study was a weight loss intervention trial with a prescribed energy deficit of approximately 500 kcal/d. Levels of 22 miRNAs were determined in serum samples from 85 participants with overweight or obesity. miRNAs were analyzed using TaqMan Array miRNA Cards and normalized to the geometric mean of spiked-in ath-miR-159a and U6 small nuclear RNA using the ΔCT method. RESULTS: The average weight loss was 5.7 kg (P < 0.001). miR-122-5p (-0.18 ± 0.06 log fold relative to initial, P < 0.01) and miR-193a-5p (-0.12 ± 0.04, P < 0.01) levels decreased in response to weight loss. miR-126a-3p (0.11 ± 0.04, P = 0.01) and miR-222-3p (1.51 ± 0.12, P < 0.001) levels increased. Furthermore, a higher level of miR-122-5p was observed at baseline in participants with the metabolic syndrome compared with participants without (0.28 ± 0.08, P < 0.01). CONCLUSIONS: Changes in circulating miR-122-5p, miR-126a-3p, miR-193a-5p, and miR-222-3p in response to diet-induced weight loss are demonstrated. Furthermore, assessment of miR-122-5p could be an indicator of an adverse metabolic health status independent of obesity.
OBJECTIVE: This study investigated whether the levels of specific serum microRNAs (miRNAs) were altered following diet-induced weight loss and whether the serum miRNAs differed in the presence of the metabolic syndrome. METHODS: The study was a weight loss intervention trial with a prescribed energy deficit of approximately 500 kcal/d. Levels of 22 miRNAs were determined in serum samples from 85 participants with overweight or obesity. miRNAs were analyzed using TaqMan Array miRNA Cards and normalized to the geometric mean of spiked-in ath-miR-159a and U6 small nuclear RNA using the ΔCT method. RESULTS: The average weight loss was 5.7 kg (P < 0.001). miR-122-5p (-0.18 ± 0.06 log fold relative to initial, P < 0.01) and miR-193a-5p (-0.12 ± 0.04, P < 0.01) levels decreased in response to weight loss. miR-126a-3p (0.11 ± 0.04, P = 0.01) and miR-222-3p (1.51 ± 0.12, P < 0.001) levels increased. Furthermore, a higher level of miR-122-5p was observed at baseline in participants with the metabolic syndrome compared with participants without (0.28 ± 0.08, P < 0.01). CONCLUSIONS: Changes in circulating miR-122-5p, miR-126a-3p, miR-193a-5p, and miR-222-3p in response to diet-induced weight loss are demonstrated. Furthermore, assessment of miR-122-5p could be an indicator of an adverse metabolic health status independent of obesity.
Authors: Anja Elaine Sørensen; Mireille N M van Poppel; Gernot Desoye; Peter Damm; David Simmons; Dorte Møller Jensen; Louise Torp Dalgaard Journal: Cells Date: 2021-01-15 Impact factor: 6.600
Authors: G Catanzaro; T Filardi; C Sabato; A Vacca; S Migliaccio; S Morano; E Ferretti Journal: J Endocrinol Invest Date: 2020-10-28 Impact factor: 4.256
Authors: Susana Sangiao-Alvarellos; Konstantinos Theofilatos; Temo Barwari; Clemens Gutmann; Kaloyan Takov; Bhawana Singh; Paula Juiz-Valiña; Bárbara María Varela-Rodríguez; Elena Outeiriño-Blanco; Elisa Duregotti; Anna Zampetaki; Lukas Lunger; Christoph Ebenbichler; Herbert Tilg; María Jesús García-Brao; Peter Willeit; Enrique Mena; Stefan Kiechl; Fernando Cordido; Manuel Mayr Journal: BMJ Open Diabetes Res Care Date: 2020-10