Alina Bisag1,2, Cristiana Bucci1,2,3,4, Sara Coluccelli1,2,3,5,6, Giulia Girolimetti2,3,6, Romolo Laurita1,2,7, Pierandrea De Iaco2,3,5, Anna Myriam Perrone2,5, Matteo Gherardi1,2,7, Lorena Marchio2,3,6, Anna Maria Porcelli2,4,8, Vittorio Colombo1,2,7,9, Giuseppe Gasparre2,3,6. 1. Department of Industrial Engineering, Alma Mater Studiorum-University of Bologna, 40136 Bologna, Italy. 2. Centro di Studio e Ricerca sulle Neoplasie Ginecologiche, Alma Mater Studiorum-University of Bologna, 40138 Bologna, Italy. 3. Department of Medical and Surgical Sciences, Alma Mater Studiorum-University of Bologna, 40138 Bologna, Italy. 4. Department of Pharmacy and Biotechnology, Alma Mater Studiorum-University of Bologna, 40126 Bologna, Italy. 5. Unit of Gynecologic Oncology, S. Orsola-Malpighi Hospital, 40138 Bologna, Italy. 6. Centre for Applied Biomedical Research, Alma Mater Studiorum-University of Bologna, 40138 Bologna, Italy. 7. Interdepartmental Center for Industrial Research Advanced Mechanical Engineering Applications and Materials Technology, Alma Mater Studiorum-University of Bologna, 40136 Bologna, Italy. 8. Interdepartmental Center for Industrial Research Life Sciences and Technologies for Health, Alma Mater Studiorum-University of Bologna, 40064 Ozzano dell'Emilia, Italy. 9. Interdepartmental Center for Industrial Research Agrifood, Alma Mater Studiorum-University of Bologna, 40126 Bologna, Italy.
Abstract
Epithelial Ovarian Cancer (EOC) is one of the leading causes of cancer-related deaths among women and is characterized by the diffusion of nodules or plaques from the ovary to the peritoneal surfaces. Conventional therapeutic options cannot eradicate the disease and show low efficacy against resistant tumor subclones. The treatment of liquids via cold atmospheric pressure plasma enables the production of plasma-activated liquids (PALs) containing reactive oxygen and nitrogen species (RONS) with selective anticancer activity. Thus, the delivery of RONS to cancer tissues by intraperitoneal washing with PALs might be an innovative strategy for the treatment of EOC. In this work, plasma-activated Ringer's Lactate solution (PA-RL) was produced by exposing a liquid substrate to a multiwire plasma source. Subsequently, PA-RL dilutions are used for the treatment of EOC, non-cancer and fibroblast cell lines, revealing a selectivity of PA-RL, which induces a significantly higher cytotoxic effect in EOC with respect to non-cancer cells.
pan class="Disease">Epithelial Ovarian Cancer (n>n class="Disease">EOC) is one of the leading causes of cancer-related deaths among women and is characterized by the diffusion of nodules or plaques from the ovary to the peritoneal surfaces. Conventional therapeutic options cannot eradicate the disease and show low efficacy against resistant tumor subclones. The treatment of liquids via cold atmospheric pressure plasma enables the production of plasma-activated liquids (PALs) containing reactive oxygen and nitrogen species (RONS) with selective anticancer activity. Thus, the delivery of RONS to cancer tissues by intraperitoneal washing with PALs might be an innovative strategy for the treatment of EOC. In this work, plasma-activated Ringer's Lactate solution (PA-RL) was produced by exposing a liquid substrate to a multiwire plasma source. Subsequently, PA-RL dilutions are used for the treatment of EOC, non-cancer and fibroblast cell lines, revealing a selectivity of PA-RL, which induces a significantly higher cytotoxic effect in EOC with respect to non-cancer cells.
Authors: Hager Mohamed; Eric Gebski; Rufranshell Reyes; Samuel Beane; Brian Wigdahl; Fred C Krebs; Katharina Stapelmann; Vandana Miller Journal: Cancers (Basel) Date: 2021-05-18 Impact factor: 6.639
Authors: Pavol Zubor; Yun Wang; Alena Liskova; Marek Samec; Lenka Koklesova; Zuzana Dankova; Anne Dørum; Karol Kajo; Dana Dvorska; Vincent Lucansky; Bibiana Malicherova; Ivana Kasubova; Jan Bujnak; Milos Mlyncek; Carlos Alberto Dussan; Peter Kubatka; Dietrich Büsselberg; Olga Golubnitschaja Journal: Int J Mol Sci Date: 2020-10-27 Impact factor: 5.923