Joseph Selvin1, Debanjana Maity2, Arya Sajayan3, George Seghal Kiran4. 1. Department of Microbiology, Pondicherry University, Puducherry, India. 2. KIIT school of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar, India. 3. Department of Food Science and Technology, Pondicherry University, Puducherry, India. 4. Department of Food Science and Technology, Pondicherry University, Puducherry, India. Electronic address: seghalkiran@gmail.com.
Abstract
OBJECTIVES: The aim of this study was to evaluate antibiotic susceptibility patterns in commercially available dietary and probiotic supplements. METHODS: Probiotic strains were isolated from the dietary supplements (designated as sample B, D and V), and multidrug resistance profiles were tested using the Kirby-Bauer test. Minimum inhibitory concentrations and double-disk synergy tests were performed to detect the mechanism of action of the resistance, and the presence of extended spectrum β-lactamase activity (ESBL) was confirmed. RESULTS: The isolates Streptococcus faecalis and Bacillus mesentericus (both from sample B) were found to be resistant to penicillin G, Lactobacillus acidophilus (sample D) was resistant to ampicillin, and all the isolates from samples B, D and V were resistant to ceftazidime. The isolates Lactobacillus sporogenes, S. faecalis, B. mesentericus from sample B, Lactobacillus. rhamnosus, Saccharomyces boulardii from sample D, and L. sporogenes (sample V) were resistant to erythromycin. CONCLUSIONS: The findings showed the presence of antibiotic resistance in probiotic bacteria isolated from commercially available dietary supplements. Because multidrug resistance is a serious emerging issue, and the risk of drug-resistant gene transfer to commensals or pathogens of the gut is inevitable, the safety of probiotics has become a major criterion of interest. The findings of this study would serve as a platform for further screening and characterization of the determinants of antibiotic resistance and the genetic mechanisms of resistance.
OBJECTIVES: The aim of this study was to evaluate antibiotic susceptibility patterns in commercially available dietary and probiotic supplements. METHODS: Probiotic strains were isolated from the dietary supplements (designated as sample B, D and V), and multidrug resistance profiles were tested using the Kirby-Bauer test. Minimum inhibitory concentrations and double-disk synergy tests were performed to detect the mechanism of action of the resistance, and the presence of extended spectrum β-lactamase activity (ESBL) was confirmed. RESULTS: The isolates Streptococcus faecalis and Bacillus mesentericus (both from sample B) were found to be resistant to penicillin G, Lactobacillus acidophilus (sample D) was resistant to ampicillin, and all the isolates from samples B, D and V were resistant to ceftazidime. The isolates Lactobacillus sporogenes, S. faecalis, B. mesentericus from sample B, Lactobacillus. rhamnosus, Saccharomyces boulardii from sample D, and L. sporogenes (sample V) were resistant to erythromycin. CONCLUSIONS: The findings showed the presence of antibiotic resistance in probiotic bacteria isolated from commercially available dietary supplements. Because multidrug resistance is a serious emerging issue, and the risk of drug-resistant gene transfer to commensals or pathogens of the gut is inevitable, the safety of probiotics has become a major criterion of interest. The findings of this study would serve as a platform for further screening and characterization of the determinants of antibiotic resistance and the genetic mechanisms of resistance.
Authors: Anna-Ursula Happel; Brian R Kullin; Hoyam Gamieldien; Heather B Jaspan; Arvind Varsani; Darren Martin; Jo-Ann S Passmore; Rémy Froissart Journal: Microorganisms Date: 2022-01-20
Authors: Daniela Machado; Joana Cristina Barbosa; Diana Almeida; José Carlos Andrade; Ana Cristina Freitas; Ana Maria Gomes Journal: Int J Environ Res Public Health Date: 2022-07-27 Impact factor: 4.614