Jaciara Neves Sousa1, Alanna Fernandes Paraíso2, João Marcus Oliveira Andrade3, Deborah Farias Lelis1, Eloá Mangabeira Santos3, Juliana Pinto Lima4, Renato Sobral Monteiro-Junior1, Marcos Flávio Silveira Vasconcelos D'Angelo1, Alfredo Mauricio Batista de Paula1, André Luiz Sena Guimarães1, Sérgio Henrique Sousa Santos5. 1. Laboratory of Health Science, Post graduate Program in Health Science, Universidade Estadual de Montes Claros (Unimontes), Minas Gerais, Brazil. 2. Departament of Nursing, Faculdades Santo Agostinho, Montes Claros, Minas Gerais, Brazil. 3. Laboratory of Health Science, Post graduate Program in Health Science, Universidade Estadual de Montes Claros (Unimontes), Minas Gerais, Brazil; Departament of Nursing, Faculdades Santo Agostinho, Montes Claros, Minas Gerais, Brazil. 4. Institute of Agricultural Sciences (ICA), Food Engineering, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil. 5. Laboratory of Health Science, Post graduate Program in Health Science, Universidade Estadual de Montes Claros (Unimontes), Minas Gerais, Brazil; Institute of Agricultural Sciences (ICA), Food Engineering, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil. Electronic address: sergiosousas@hotmail.com.
Abstract
INTRODUCTION: Gallic acid (GA) is a natural endogenous polyphenol found in a variety of fruits, vegetables and wines, with beneficial effects on the energetic homeostasis. AIM: The present study aimed to investigate oral gallic acid effects on liver steatosis and hepatic lipogenesis markers in obese mice evaluating new possible molecular related mechanisms. METHODS: Twenty-four Swiss male mice were divided into four groups and fed for 60 days with standard diet (ST), standard diet plus gallic acid (ST + GA), high-fat diet (HFD), and high-fat diet plus gallic acid (HFD + GA). We evaluated the relationship between body weight, food intake and serum levels of total cholesterol, triglycerides, insulin, aspartate and alanine transaminases. Liver histology was analyzed by hematoxylin and eosin staining. These results were accompanied by bioinformatics analyses. The acetyl-CoA carboxylase (ACC), sterol regulatory element binding protein-1 (SREBP-1) and fatty acid synthase (FAS) expression was assessed by quantitative real-time reverse transcriptase PCR (qRT-PCR). RESULTS: The main findings of the present study showed that GA reduced liver steatosis, body weight and plasma insulin levels. Analyzes of hepatic steatosis related genes expression showed that ACC and FAS mRNA were significantly suppressed in liver of HFD + GA mice. These data was corroborated by bioinformatics analysis. CONCLUSION: These data suggest an important clinical application of GA in the prevention and treatment of liver diseases.
INTRODUCTION:Gallic acid (GA) is a natural endogenous polyphenol found in a variety of fruits, vegetables and wines, with beneficial effects on the energetic homeostasis. AIM: The present study aimed to investigate oral gallic acid effects on liver steatosis and hepatic lipogenesis markers in obesemice evaluating new possible molecular related mechanisms. METHODS: Twenty-four Swiss male mice were divided into four groups and fed for 60 days with standard diet (ST), standard diet plus gallic acid (ST + GA), high-fat diet (HFD), and high-fat diet plus gallic acid (HFD + GA). We evaluated the relationship between body weight, food intake and serum levels of total cholesterol, triglycerides, insulin, aspartate and alanine transaminases. Liver histology was analyzed by hematoxylin and eosin staining. These results were accompanied by bioinformatics analyses. The acetyl-CoA carboxylase (ACC), sterol regulatory element binding protein-1 (SREBP-1) and fatty acid synthase (FAS) expression was assessed by quantitative real-time reverse transcriptase PCR (qRT-PCR). RESULTS: The main findings of the present study showed that GA reduced liver steatosis, body weight and plasma insulin levels. Analyzes of hepatic steatosis related genes expression showed that ACC and FAS mRNA were significantly suppressed in liver of HFD + GAmice. These data was corroborated by bioinformatics analysis. CONCLUSION: These data suggest an important clinical application of GA in the prevention and treatment of liver diseases.