Mohsin Khurshid1, Muhammad Hidayat Rasool2, Muhammad Hussnain Siddique3, Farrukh Azeem4, Muhammad Naeem5, Muhammad Sohail6, Muhammad Sarfraz7, Muhammad Saqalein8, Zeeshan Taj9, Muhammad Atif Nisar10, Muhammad Usman Qamar11, Asim Shahzad12. 1. Department of Microbiology, Government College University, Faisalabad, Pakistan. mohsin.mic@gmail.com. 2. Department of Microbiology, Government College University, Faisalabad, Pakistan. drmhrasool@gcuf.edu.pk. 3. Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan. mhs1049@gmail.com. 4. Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan. azeuaf@hotmail.com. 5. Department of Microbiology, Government College University, Faisalabad, Pakistan. muhammadnaeem@gcuf.edu.pk. 6. Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan. drsohailmmg@gmail.com. 7. Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan. sgaundal@hotmail.com. 8. Department of Microbiology, Government College University, Faisalabad, Pakistan. drsaqalein@gcuf.edu.pk. 9. Department of Microbiology, Government College University, Faisalabad, Pakistan. drzeeshantaj@gcuf.edu.pk. 10. Department of Microbiology, Government College University, Faisalabad, Pakistan. matif100@gmail.com. 11. Department of Microbiology, Government College University, Faisalabad, Pakistan. musmanqamar@gcuf.edu.pk. 12. Department of Pathology, Faculty of Veterinary Sciences, University of Agriculture, Faisalabad, Pakistan. dr.asimshahzad@gmail.com.
Abstract
INTRODUCTION: The spread of carbapenem-resistant Acinetobacter baumannii (CRAB) is difficult to control especially in the hospitals due to the successful mobilization and evolution of the genetic elements harboring the resistant determinants. The study was conducted to examine the distribution of aminoglycosides, tetracycline, and sulfonamide-resistant determinants among CRAB isolates that carry the blaOXA-23 gene. METHODOLOGY: For a total of 160 CRAB strains isolated at tertiary care hospitals of Pakistan that mainly carried blaOXA-23 gene were included in the study to evaluate the assortment of antibiotic resistance genes. RESULTS: The susceptibility rates of CRAB for other than beta-lactam drugs were 2.5% for both ciprofloxacin and aminoglycosides and 18% and 25% for sulfonamides and tetracyclines, respectively. Polymyxin B (MIC90, 1 g/mL) Colistin (MIC90, 1 g/mL) and Tigecycline (MIC90, 2 g/mL) were most active against these extensively drug-resistant CRAB isolates. The isolates were found to possess various genes mainly the tetB and sul2 for tetracycline and sulfonamide but the genes conferring resistance to aminoglycosides were varied with various combinations. CONCLUSION: Despite the CRAB clones containing blaOXA-23 have been previously reported in Pakistani hospitals, the screening of genetic determinants responsible for other antimicrobial agents is crucial for developing an effective surveillance and mitigation system for infection management. Copyright (c) 2019 Mohsin Khurshid, Muhammad Hidayat RaMohsin Khurshid, Muhammad Hidayat Rasool, Muhammad Hussnain Siddique, Farrukh Azeem, Muhammad Naeem, Muhammad Sohail, Muhammad Sarfraz, Muhammad Saqalein, Zeeshan Taj, Muhammad Atif Nisar, Muhammad Usman Qamar, Asim Shahzad.
INTRODUCTION: The spread of carbapenem-resistant Acinetobacter baumannii (CRAB) is difficult to control especially in the hospitals due to the successful mobilization and evolution of the genetic elements harboring the resistant determinants. The study was conducted to examine the distribution of aminoglycosides, tetracycline, and sulfonamide-resistant determinants among CRAB isolates that carry the blaOXA-23 gene. METHODOLOGY: For a total of 160 CRAB strains isolated at tertiary care hospitals of Pakistan that mainly carried blaOXA-23 gene were included in the study to evaluate the assortment of antibiotic resistance genes. RESULTS: The susceptibility rates of CRAB for other than beta-lactam drugs were 2.5% for both ciprofloxacin and aminoglycosides and 18% and 25% for sulfonamides and tetracyclines, respectively. Polymyxin B (MIC90, 1 g/mL) Colistin (MIC90, 1 g/mL) and Tigecycline (MIC90, 2 g/mL) were most active against these extensively drug-resistant CRAB isolates. The isolates were found to possess various genes mainly the tetB and sul2 for tetracycline and sulfonamide but the genes conferring resistance to aminoglycosides were varied with various combinations. CONCLUSION: Despite the CRAB clones containing blaOXA-23 have been previously reported in Pakistani hospitals, the screening of genetic determinants responsible for other antimicrobial agents is crucial for developing an effective surveillance and mitigation system for infection management. Copyright (c) 2019 Mohsin Khurshid, Muhammad Hidayat RaMohsin Khurshid, Muhammad Hidayat Rasool, Muhammad Hussnain Siddique, Farrukh Azeem, Muhammad Naeem, Muhammad Sohail, Muhammad Sarfraz, Muhammad Saqalein, Zeeshan Taj, Muhammad Atif Nisar, Muhammad Usman Qamar, Asim Shahzad.
Authors: Mohsin Khurshid; Muhammad Hidayat Rasool; Usman Ali Ashfaq; Bilal Aslam; Muhammad Waseem; Muhammad Akhtar Ali; Ahmad Almatroudi; Farhan Rasheed; Muhammad Saeed; Qinglan Guo; Minggui Wang Journal: Infect Drug Resist Date: 2020-08-18 Impact factor: 4.003