David Eisenberger1, Christian Tuschak2, Markus Werner3, Christian Bogdan3, Thomas Bollinger4, Hamid Hossain5,6, Petra Friedrich7, Ziyad Hussein8, Christoph Pöhlmann9, Benjamin Würstl10, Silke Nickel1, Verena Lehner-Reindl1, Christiane Höller2, Bernhard Liebl2, Giuseppe Valenza1,3. 1. Bavarian Health and Food Safety Authority, Erlangen, Germany. 2. Bavarian Health and Food Safety Authority, Oberschleißheim, Germany. 3. Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, Germany. 4. Institut für Laboratoriumsmedizin, Mikrobiologie und Krankenhaushygiene (ILMH), Klinikum Bayreuth GmbH, Bayreuth, Germany. 5. Institut für Laboratoriumsmedizin und Mikrobiologie, Klinikum St Marien Amberg, Amberg, Germany. 6. Institut für Laboratoriumsmedizin und Mikrobiologie, Kliniken Nordoberpfalz AG, Weiden, Germany. 7. Deutsches Beratungszentrum für Hygiene BZH GmbH, Freiburg, Germany. 8. Institut für Laboratoriumsdiagnostik und Transfusionsmedizin, DONAUISAR Klinikum, Deggendorf, Germany. 9. Abteilung für Hygiene und Mikrobiologie, Klinikum Memmingen, Memmingen, Germany. 10. Stabsstelle Krankenhaushygiene und Infektionsprävention, München Klinik, München, Germany.
Abstract
OBJECTIVES: Infections caused by vancomycin-resistant Enterococcus faecium (VREfm) represent a major public health concern due to limited treatment options. Among invasive isolates of VREfm, ST117, ST80 and ST78 represent the most frequently detected STs by MLST in Germany. In this study, we investigated the genetic diversity of isolates of VREfm recovered from different nosocomial outbreaks in Bavaria, Germany, by WGS. METHODS: Between January 2018 and April 2019, 99 non-replicate isolates of VREfm originating from nosocomial outbreaks at eight different hospitals in Bavaria were investigated for genetic diversity by WGS. In detail, complex types (CTs) were identified by core-genome MLST. Furthermore, an SNP analysis was performed for all VREfm strains. RESULTS: Most of the isolates of this study (76%) belonged to three major clonal groups, which occurred in at least three hospitals: ST80/CT1065 vanB (n = 45; six hospitals), ST117/CT71 vanB (n = 11; four hospitals) and ST78/CT894like vanA (n = 19; three hospitals). Moreover, isolates of the predominant lineage ST80/CT1065 vanB showed a maximum difference of 36 SNPs as revealed by SNP analysis. CONCLUSIONS: Whole-genome analysis of VREfm causing nosocomial outbreaks suggests the occurrence of few endemic clonal lineages in Bavarian hospital settings, namely ST80/CT1065 vanB, ST117/CT71 vanB and ST78/CT894like vanA. Further studies are needed for a better understanding of the factors affecting the successful spread of the above-mentioned lineages.
OBJECTIVES: Infections caused by vancomycin-resistant Enterococcus faecium (VREfm) represent a major public health concern due to limited treatment options. Among invasive isolates of VREfm, ST117, ST80 and ST78 represent the most frequently detected STs by MLST in Germany. In this study, we investigated the genetic diversity of isolates of VREfm recovered from different nosocomial outbreaks in Bavaria, Germany, by WGS. METHODS: Between January 2018 and April 2019, 99 non-replicate isolates of VREfm originating from nosocomial outbreaks at eight different hospitals in Bavaria were investigated for genetic diversity by WGS. In detail, complex types (CTs) were identified by core-genome MLST. Furthermore, an SNP analysis was performed for all VREfm strains. RESULTS: Most of the isolates of this study (76%) belonged to three major clonal groups, which occurred in at least three hospitals: ST80/CT1065 vanB (n = 45; six hospitals), ST117/CT71 vanB (n = 11; four hospitals) and ST78/CT894like vanA (n = 19; three hospitals). Moreover, isolates of the predominant lineage ST80/CT1065 vanB showed a maximum difference of 36 SNPs as revealed by SNP analysis. CONCLUSIONS: Whole-genome analysis of VREfm causing nosocomial outbreaks suggests the occurrence of few endemic clonal lineages in Bavarian hospital settings, namely ST80/CT1065 vanB, ST117/CT71 vanB and ST78/CT894like vanA. Further studies are needed for a better understanding of the factors affecting the successful spread of the above-mentioned lineages.
Authors: Michaël Timmermans; Bert Bogaerts; Kevin Vanneste; Sigrid C J De Keersmaecker; Nancy H C Roosens; Carole Kowalewicz; Guillaume Simon; Maria A Argudín; Ariane Deplano; Marie Hallin; Pierre Wattiau; David Fretin; Olivier Denis; Cécile Boland Journal: J Antimicrob Chemother Date: 2021-12-24 Impact factor: 5.790
Authors: Lindsay A Rogers; Kayla Strong; Susan C Cork; Tim A McAllister; Karen Liljebjelke; Rahat Zaheer; Sylvia L Checkley Journal: Front Public Health Date: 2021-06-10
Authors: Carlos L Correa-Martínez; Annette Jurke; Janne Schmitz; Frieder Schaumburg; Stefanie Kampmeier; Alexander Mellmann Journal: Microorganisms Date: 2022-01-08
Authors: Ilona Trautmannsberger; Laura Kolberg; Melanie Meyer-Buehn; Johannes Huebner; Guido Werner; Robert Weber; Valerie Heselich; Sebastian Schroepf; Hans-Georg Muench; Ulrich von Both Journal: Antimicrob Resist Infect Control Date: 2022-03-12 Impact factor: 4.887
Authors: Linda Falgenhauer; Ingeborg Preuser; Can Imirzalioglu; Jane Falgenhauer; Moritz Fritzenwanker; Dietrich Mack; Christoph Best; Ursel Heudorf; Trinad Chakraborty Journal: Infect Prev Pract Date: 2021-03-27