Literature DB >> 3208121

Human neuronal cell viability demonstrated in culture after cryopreservation.

V Silani1, A Pizzuti, O Strada, A Falini, M Buscaglia, G Scarlato.   

Abstract

A human neuronal cell freezing technique has been developed. The results indicate that human fetal neuronal cells can be frozen with 7%-10% dimethyl sulfoxide (DMSO) as cryoprotectant. The survival of isolated thawed cells has been evaluated in culture. Cells have been frozen at -196 degrees C for 370 days without loss of viability. Average recovery rate of frozen cells was 62% of the recovery rate of cultured unfrozen controls. Thawed cells show neurite outgrowth and maintain both cellular markers such as neuron specific enolase (NSE) and neurochemical characteristics (GABA synthesis). Morphological integrity of cryopreserved neurons has been confirmed at ultrastructural level.

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Year:  1988        PMID： 3208121     DOI： 10.1016/0006-8993(88)90331-9

Source DB:  PubMed          Journal:  Brain Res        ISSN： 0006-8993            Impact factor:   3.252


  10 in total

1.  Cryopreservation, survival and function of intrastriatal fetal mesencephalic grafts in a rat model of Parkinson's disease.

Authors:  H Sauer; E M Frodl; A Kupsch; G ten Bruggencate; W H Oertel
Journal:  Exp Brain Res       Date:  1992       Impact factor: 1.972

2.  The development and characterisation of complex ovine neuron cultures from fresh and frozen foetal neurons.

Authors:  Graham W Kay; Manfred J Oswald; David N Palmer
Journal:  J Neurosci Methods       Date:  2006-02-17       Impact factor: 2.390

3.  Cryopreservation and culture of the human fetal brain tissues.

Authors:  R S Cai; D L Xue; X H Jiang
Journal:  J Tongji Med Univ       Date:  1993

4.  Freshly frozen E18 rat cortical cells can generate functional neural networks after standard cryopreservation and thawing procedures.

Authors:  Kim Quasthoff; Stefano Ferrea; Wiebke Fleischer; Stephan Theiss; Alfons Schnitzler; Marcel Dihné; Janine Walter
Journal:  Cytotechnology       Date:  2014-02-23       Impact factor: 2.058

5.  Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model.

Authors:  Bing Yang; Kaushik Parsha; Krystal Schaar; Nikunj Satani; Xiaopei Xi; Jaroslaw Aronowski; Sean I Savitz
Journal:  Stem Cells Int       Date:  2016-06-15       Impact factor: 5.443

6.  Cryopreservation of GABAergic Neuronal Precursors for Cell-Based Therapy.

Authors:  Daniel Rodríguez-Martínez; María Magdalena Martínez-Losa; Manuel Alvarez-Dolado
Journal:  PLoS One       Date:  2017-01-25       Impact factor: 3.240

7.  The Effect of Hypothermic and Cryogenic Preservation on Engineered Neural Tissue.

Authors:  Adam G E Day; Kulraj Singh Bhangra; Celia Murray-Dunning; Lara Stevanato; James B Phillips
Journal:  Tissue Eng Part C Methods       Date:  2017-10       Impact factor: 3.056

8.  Cryopreservation of Human Midbrain Dopaminergic Neural Progenitor Cells Poised for Neuronal Differentiation.

Authors:  Nicola J Drummond; Karamjit Singh Dolt; Maurice A Canham; Peter Kilbride; G John Morris; Tilo Kunath
Journal:  Front Cell Dev Biol       Date:  2020-11-05

9.  Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research.

Authors:  Balmiki Ray; Nipun Chopra; Justin M Long; Debomoy K Lahiri
Journal:  Mol Brain       Date:  2014-09-16       Impact factor: 4.041

10.  A non-aggressive, highly efficient, enzymatic method for dissociation of human brain-tumors and brain-tissues to viable single-cells.

Authors:  Ilan Volovitz; Netanel Shapira; Haim Ezer; Aviv Gafni; Merav Lustgarten; Tal Alter; Idan Ben-Horin; Ori Barzilai; Tal Shahar; Andrew Kanner; Itzhak Fried; Igor Veshchev; Rachel Grossman; Zvi Ram
Journal:  BMC Neurosci       Date:  2016-06-01       Impact factor: 3.288
  10 in total

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