Natalya Kumskova1, Yulia Ermolenko1,2, Nadezhda Osipova1,2, Aleksey Semyonkin1,3, Natalia Kildeeva4, Marina Gorshkova5, Andrey Kovalskii6, Tatyana Kovshova1,7, Vadim Tarasov3, Joerg Kreuter3,8, Olga Maksimenko1,2, Svetlana Gelperina1,2. 1. D. Mendeleev University of Chemical Technology of Russia, Moscow, Russia. 2. Drugs Technology LLC, Khimki, Russia. 3. I. M. Sechenov First Moscow State Medical University, Moscow, Russia. 4. A. N. Kosygin Russian State University, Moscow, Russia. 5. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Moscow, Russia. 6. National University of Science and Technology "MISIS", Moscow, Russia. 7. Lomonosov Moscow State University, Moscow, Russia. 8. Institute of Pharmaceutical Technology, Biocenter Niederursel, Goethe University, Frankfurt/Main, Germany.
Abstract
Aims: To evaluate the influence of minor differences in molecular weights of commercially available low molecular weight PLGA grades on the kinetics of doxorubicin release from the nanoparticles. Methods: Three low-molecular weight 50/50 PLGA polymers were thoroughly characterised concerning intrinsic viscosity, molecular weight (Mw), acid value, and residual monomer content. The doxorubicin-loaded nanoparticles prepared using these polymers were evaluated concerning the kinetics of drug release and hydrolytic degradation. Results: The Mw of the polymers were slightly different: 10.2, 10.3, and 4.7 kDa. The nanoparticles obtained from the polymer with Mw of 4.7 kDa exhibited considerably higher rates of drug release and polymer degradation. Conclusion: In the case of low molecular weight PLGA grades even a few kilodaltons could be important for the batch-to-batch reproducibility of the nanoformulation parameters. These results bring forward the importance of in-house characterisation of the polymers to be used for the nanoparticle preparation.
Aims: To evaluate the influence of minor differences in molecular weights of commercially available low molecular weight PLGA grades on the kinetics of doxorubicin release from the nanoparticles. Methods: Three low-molecular weight 50/50 PLGA polymers were thoroughly characterised concerning intrinsic viscosity, molecular weight (Mw), acid value, and residual monomer content. The doxorubicin-loaded nanoparticles prepared using these polymers were evaluated concerning the kinetics of drug release and hydrolytic degradation. Results: The Mw of the polymers were slightly different: 10.2, 10.3, and 4.7 kDa. The nanoparticles obtained from the polymer with Mw of 4.7 kDa exhibited considerably higher rates of drug release and polymer degradation. Conclusion: In the case of low molecular weight PLGA grades even a few kilodaltons could be important for the batch-to-batch reproducibility of the nanoformulation parameters. These results bring forward the importance of in-house characterisation of the polymers to be used for the nanoparticle preparation.