Chenxi Zhang1,2, Bin Zhang1,2, Baiyin Yuan3, Caiping Chen4, Ying Zhou1,2, Yu Zhang5,6, Zhihong Sheng1,2, Nan Sun1,2, Xiaoyuan Wu1,2. 1. Central Laboratory, Nanjing Chest Hospital, Medical School of Southeast University, Nanjing, PR China. 2. Central Laboratory, Nanjing Brain Hospital, Nanjing Medical University, Nanjing, PR China. 3. Biomedical Research Institute, College of Life Science and Health, Wuhan University of Science and Technology, Wuhan, PR China. 4. Jiangsu Key Laboratory of Drug Discovery for Metabolic Disease and State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, PR China. 5. Department of Respiratory Medicine, Nanjing Chest Hospital, Medical School of Southeast University, Nanjing, PR China. 6. Department of Respiratory Medicine, Nanjing Brain Hospital, Nanjing Medical University, Nanjing, PR China.
Abstract
Aim: We aimed to explore the circular RNA (circRNA) profile of small-cell lung cancer (SCLC). Materials & methods: Total RNA was extracted from six paired SCLC tumors and adjacent noncancerous tissues. Next-generation sequencing was performed to identify the circRNA expression profile of SCLC. Results: We found that five circRNAs were significantly upregulated and 30 circRNAs were significantly downregulated in the SCLC tissues. We confirmed the five upregulated and four randomly selected downregulated circRNAs using real-time quantitative PCR. Notably, circ-STXBP5L was selectively upregulated in SCLC samples, but undetectable in the normal control tissues. Bioinformatics analysis demonstrated that circ-STXBP5L may participate in SCLC carcinogenesis by regulating numerous cancer-related pathways. Conclusion: This study may provide new insights into the early diagnosis and development of targeted therapies for SCLC.
Aim: We aimed to explore the circular RNA (circRNA) profile of small-cell lung cancer (SCLC). Materials & methods: Total RNA was extracted from six paired SCLC tumors and adjacent noncancerous tissues. Next-generation sequencing was performed to identify the circRNA expression profile of SCLC. Results: We found that five circRNAs were significantly upregulated and 30 circRNAs were significantly downregulated in the SCLC tissues. We confirmed the five upregulated and four randomly selected downregulated circRNAs using real-time quantitative PCR. Notably, circ-STXBP5L was selectively upregulated in SCLC samples, but undetectable in the normal control tissues. Bioinformatics analysis demonstrated that circ-STXBP5L may participate in SCLC carcinogenesis by regulating numerous cancer-related pathways. Conclusion: This study may provide new insights into the early diagnosis and development of targeted therapies for SCLC.