OBJECTIVE: to investigate Leishmania species in a series of autochthonous cutaneous leishmaniasis (CL) cases in Amapá State, Brazilian Amazon. METHODS: this was a descriptive ecological study carried out from January-October/2018 at a reference center for CL diagnosis in Amapá; individuals with CL receiving care from January-May/2018 were recruited; clinical data and skin biopsies were obtained; from extracted DNA (phenol-chloroform) we amplified the hsp70-234 gene region (PCR) for nucleotide sequencing (Applied Biosystems: ABI3500XL). RESULTS: 38 individuals were interviewed, examined and diagnosed; men predominated (28/38; mean age=32.5±11.3); lesions (most ulcers: 37/38) measuring 0,4-10mm (34/38) and ≥11mm (4/38) were multiple in 20/38 individuals; diagnosis of L. braziliensis (1), L. naiffi (1), L. infantum (1), L. (Viannia) sp. (1), L. amazonensis (2) and L. guyanensis (32); individuals infected with L. guyanensis (32/38) lived in 9/10 municipalities represented in the sample, and 17/32 of these had multiple lesions. CONCLUSION: presence of Leishmania guyanensis predominated and was frequently associated with multiple lesions.
OBJECTIVE: to investigate Leishmania species in a series of autochthonous cutaneous leishmaniasis (CL) cases in Amapá State, Brazilian Amazon. METHODS: this was a descriptive ecological study carried out from January-October/2018 at a reference center for CL diagnosis in Amapá; individuals with CL receiving care from January-May/2018 were recruited; clinical data and skin biopsies were obtained; from extracted DNA (phenol-chloroform) we amplified the hsp70-234 gene region (PCR) for nucleotide sequencing (Applied Biosystems: ABI3500XL). RESULTS: 38 individuals were interviewed, examined and diagnosed; men predominated (28/38; mean age=32.5±11.3); lesions (most ulcers: 37/38) measuring 0,4-10mm (34/38) and ≥11mm (4/38) were multiple in 20/38 individuals; diagnosis of L. braziliensis (1), L. naiffi (1), L. infantum (1), L. (Viannia) sp. (1), L. amazonensis (2) and L. guyanensis (32); individuals infected with L. guyanensis (32/38) lived in 9/10 municipalities represented in the sample, and 17/32 of these had multiple lesions. CONCLUSION: presence of Leishmania guyanensis predominated and was frequently associated with multiple lesions.
Authors: Minelly A da Silva; Harold H Fokoue; Saara N Fialho; Ana Paula de A Dos Santos; Norton R D L P Rossi; Aurileya de J Gouveia; Amália S Ferreira; Guilherme M Passarini; Ana F G Garay; Jorge J Alfonso; Andreimar M Soares; Fernando B Zanchi; Massuo J Kato; Carolina B G Teles; Christian C Kuehn Journal: Parasitol Res Date: 2021-05-08 Impact factor: 2.289
Authors: Luciana P Oliveira; Luciana C S Nascimento; Fabiola S Santos; Jaqueline L C Takamatsu; Luiz R P Sanchez; Walter S Santos; Lourdes M Garcez Journal: Int J Environ Res Public Health Date: 2022-05-23 Impact factor: 4.614
Authors: Francisco J A Santos; Luciana C S Nascimento; Wellington B Silva; Luciana P Oliveira; Walter S Santos; Délia C F Aguiar; Lourdes M Garcez Journal: Int J Environ Res Public Health Date: 2020-11-16 Impact factor: 3.390