Vivian Carolina Salgueiro1, Milena D'Angelo Lima Seixas2, Lorrayne Cardoso Guimarães3, Dennis de Carvalho Ferreira4, Denise Cotrim Da Cunha5, Simone Aranha Nouér6, Kátia Regina Netto Dos Santos7. 1. Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. vivi.salgueiro@gmail.com. 2. Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. milenaalima@yahoo.com.br. 3. Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. lorraynecardog@yahoo.com.br. 4. Faculdade de Odontologia da Universidade Estácio de Sá, Rio de Janeiro, Brazil. denniscf@gmail.com. 5. Maternidade Leila Diniz, Hospital Municipal Lourenço Jorge, Rio de Janeiro, Brazil. decotrim@globo.com. 6. Hospital Universitário Clementino Fraga Filho, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. snouer@hucff.ufrj.br. 7. Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. santoskrn@micro.ufrj.br.
Abstract
INTRODUCTION: Staphylococcal colonization is a risk factor for healthcare-associated infections, which are frequent in Neonatal Intensive Care Units (NICU). This study analyzed microbiology, epidemiology and clinical aspects of Staphylococcus spp. colonizing neonates. METHODOLOGY: Nasal or periumbilical swabs were evaluated from 175 newborns admitted to a NICU of a Rio de Janeiro hospital from March to September 2009. Clinical data were obtained from the medical records. SCCmec typing and the mecA and Panton-Valentine Leukocidin (PVL) genes were detected by PCR. Clonal diversity was evaluated by pulsed-field gel electrophoresis. RESULTS: Staphylococcus spp. isolates were detected in 98 (56%) neonates, 66.3% of them had birth weight ≤ 2500 g, 62.2% were preterm (˂ 37 weeks) and the mean length of hospitalization was 14.9 days. Among the 133 isolates identified, 48.1% were S. epidermidis, 23.3% S. haemolyticus and 13.5% S. aureus. Methicillin-resistant Staphylococcus isolate was detected in 77.6% of neonates. The methicillin-resistant S. aureus isolates carried the SCCmec type IV, while 94.6% of S. epidermidis and 85.7% of S. haemolyticus presented non-typeable cassettes. Among the S. aureus, 55.6% had PVL genes and the USA800 genotype was prevalent. Two genotypes of S. epidermidis and one of S. haemolyticus clustered 42.2% and 25.8% of the isolates, respectively. S haemolyticus colonization was associated with the use of parenteral nutrition and mechanical ventilation. CONCLUSION: High rate of neonates colonized by methicillin-resistant Staphylococcus species and the permanence of clones circulating in the NICU highlight the importance for continuous and preventive surveillance in this high-risk population. Copyright (c) 2019 Katia Regina Netto dos Santos, Vivian Carolina Salgueiro, Milena D’Angelo Lima Seixas, Lorrayne Cardoso Guimaraes, Dennis de Carvalho Ferreira, Denise Cotrim da Cunha, Simone Aranha Nouer.
INTRODUCTION: Staphylococcal colonization is a risk factor for healthcare-associated infections, which are frequent in Neonatal Intensive Care Units (NICU). This study analyzed microbiology, epidemiology and clinical aspects of Staphylococcus spp. colonizing neonates. METHODOLOGY: Nasal or periumbilical swabs were evaluated from 175 newborns admitted to a NICU of a Rio de Janeiro hospital from March to September 2009. Clinical data were obtained from the medical records. SCCmec typing and the mecA and Panton-Valentine Leukocidin (PVL) genes were detected by PCR. Clonal diversity was evaluated by pulsed-field gel electrophoresis. RESULTS:Staphylococcus spp. isolates were detected in 98 (56%) neonates, 66.3% of them had birth weight ≤ 2500 g, 62.2% were preterm (˂ 37 weeks) and the mean length of hospitalization was 14.9 days. Among the 133 isolates identified, 48.1% were S. epidermidis, 23.3% S. haemolyticus and 13.5% S. aureus. Methicillin-resistant Staphylococcus isolate was detected in 77.6% of neonates. The methicillin-resistant S. aureus isolates carried the SCCmec type IV, while 94.6% of S. epidermidis and 85.7% of S. haemolyticus presented non-typeable cassettes. Among the S. aureus, 55.6% had PVL genes and the USA800 genotype was prevalent. Two genotypes of S. epidermidis and one of S. haemolyticus clustered 42.2% and 25.8% of the isolates, respectively. S haemolyticus colonization was associated with the use of parenteral nutrition and mechanical ventilation. CONCLUSION: High rate of neonates colonized by methicillin-resistant Staphylococcus species and the permanence of clones circulating in the NICU highlight the importance for continuous and preventive surveillance in this high-risk population. Copyright (c) 2019 Katia Regina Netto dos Santos, Vivian Carolina Salgueiro, Milena D’Angelo Lima Seixas, Lorrayne Cardoso Guimaraes, Dennis de Carvalho Ferreira, Denise Cotrim da Cunha, Simone Aranha Nouer.
Authors: Ralciane de Paula Menezes; Lara de Andrade Marques; Felipe Flávio Silva; Nagela Bernadelli Sousa Silva; Priscila Guerino Vilela Alves; Meliza Arantes de Souza Bessa; Lúcio Borges de Araújo; Mário Paulo Amante Penatti; Reginaldo Dos Santos Pedroso; Denise Von Dolinger de Brito Röder Journal: Microorganisms Date: 2022-03-05