Jingwen Liao1, Junhao Huang1, Shen Wang1,2, Mingqiang Xiang2, Dan Wang1,2, Hailin Deng1,2, Honggang Yin1, Fengpeng Xu1, Min Hu3,4. 1. Guangdong Provincial Key Laboratory of Sports and Health Promotion, Scientific Research Center, Guangzhou Sport University, Guangzhou, China. 2. Department of Sports and Health, Guangzhou Sport University, Guangzhou, China. 3. Guangdong Provincial Key Laboratory of Sports and Health Promotion, Scientific Research Center, Guangzhou Sport University, Guangzhou, China. whoomin@aliyun.com. 4. Department of Sports and Health, Guangzhou Sport University, Guangzhou, China. whoomin@aliyun.com.
Abstract
AIMS: This study investigated the effects of exercise and diet intervention on appetite-regulating hormones and subjective appetite changes in obese children and examined expressions of specific key microRNAs (miRNA, miR). METHODS: 16 obese children were included in a training program consisting of exercise and diet intervention for 6 weeks. Before and after the intervention, fasting blood was collected to determine appetite-regulating hormones (leptin, ghrelin, and orexin) and miRNA (miR-103a-3p and miR-200a-3p) levels; eating behavior of the children was reported using the Children Eating Behavior Questionnaire (CEBQ). RESULTS: The level of orexin was significantly decreased (P < 0.05), while ghrelin was significantly enhanced (P < 0.05) after 6 weeks. The scores of food responsiveness (FR) and enjoyment of food (EF) of the CEBQ were significantly decreased (P < 0.05) after intervention. The changes of leptin and that of SR were significantly correlated (r = - 0.455, P < 0.05), and the correlation between the alterations of orexin and that of EF was moderate with significance (r = 0.625, P < 0.05). miR-103a-3p expression was not statistically changed, while miR-200a-3p was significantly inhibited after 6-week intervention (P < 0.05). The correlation between relative changes of miR-103a-3p and that of leptin and orexin were both with significant difference (r = 0.413, P < 0.05; r = 0.409, P < 0.05), whereas the alterations of miR-200a-3p were not correlative with hormones or appetite sensation. CONCLUSION: Exercise combined with diet intervention for 6 weeks was effective in regulating appetite sensations and hormones in obese children, and miR-103a-3p and miR-200a-3p might provide a foundation for target biomarkers of appetite trait in modulating the energy balance control by exercise and dietary intervention. LEVEL OF EVIDENCE: Level III, case-control analytic study. TRIAL REGISTRATION: The trial was registered in ClinicalTrials.gov (NCT03762629).
AIMS: This study investigated the effects of exercise and diet intervention on appetite-regulating hormones and subjective appetite changes in obesechildren and examined expressions of specific key microRNAs (miRNA, miR). METHODS: 16 obesechildren were included in a training program consisting of exercise and diet intervention for 6 weeks. Before and after the intervention, fasting blood was collected to determine appetite-regulating hormones (leptin, ghrelin, and orexin) and miRNA (miR-103a-3p and miR-200a-3p) levels; eating behavior of the children was reported using the Children Eating Behavior Questionnaire (CEBQ). RESULTS: The level of orexin was significantly decreased (P < 0.05), while ghrelin was significantly enhanced (P < 0.05) after 6 weeks. The scores of food responsiveness (FR) and enjoyment of food (EF) of the CEBQ were significantly decreased (P < 0.05) after intervention. The changes of leptin and that of SR were significantly correlated (r = - 0.455, P < 0.05), and the correlation between the alterations of orexin and that of EF was moderate with significance (r = 0.625, P < 0.05). miR-103a-3p expression was not statistically changed, while miR-200a-3p was significantly inhibited after 6-week intervention (P < 0.05). The correlation between relative changes of miR-103a-3p and that of leptin and orexin were both with significant difference (r = 0.413, P < 0.05; r = 0.409, P < 0.05), whereas the alterations of miR-200a-3p were not correlative with hormones or appetite sensation. CONCLUSION: Exercise combined with diet intervention for 6 weeks was effective in regulating appetite sensations and hormones in obesechildren, and miR-103a-3p and miR-200a-3p might provide a foundation for target biomarkers of appetite trait in modulating the energy balance control by exercise and dietary intervention. LEVEL OF EVIDENCE: Level III, case-control analytic study. TRIAL REGISTRATION: The trial was registered in ClinicalTrials.gov (NCT03762629).
Authors: Neil A King; Phillipa P Caudwell; Mark Hopkins; James R Stubbs; Erik Naslund; John E Blundell Journal: Am J Clin Nutr Date: 2009-08-12 Impact factor: 7.045
Authors: Sergio Chieffi; Giovanni Messina; Ines Villano; Antonietta Messina; Maria Esposito; Vincenzo Monda; Anna Valenzano; Fiorenzo Moscatelli; Teresa Esposito; Marco Carotenuto; Andrea Viggiano; Giuseppe Cibelli; Marcellino Monda Journal: Front Physiol Date: 2017-02-14 Impact factor: 4.566