Refath Farzana1, Lim S Jones2, Md Anisur Rahman3, Mark A Toleman4, Kirsty Sands5, Edward Portal6, Ian Boostrom7, Md Abul Kalam8, Brekhna Hassan9, Akm Nasir Uddin10, Timothy R Walsh11. 1. School of Medicine, Department of Medical Microbiology, Institute of Infection and Immunity, Cardiff University, Cardiff, United Kingdom. farzanar@cardiff.ac.uk. 2. Public Health Wales Microbiology, University Hospital of Wales, Cardiff, United Kingdom. lim.jones@wales.nhs.uk. 3. Department of Virology, Dhaka Medical College, Dhaka, Bangladesh. dr.shanto30@yahoo.com. 4. School of Medicine, Department of Medical Microbiology, Institute of Infection and Immunity, Cardiff University, Cardiff, United Kingdom. tolemanma@cardiff.ac.uk. 5. School of Medicine, Department of Medical Microbiology, Institute of Infection and Immunity, Cardiff University, Cardiff, United Kingdom. sandsk1@cardiff.ac.uk. 6. School of Medicine, Department of Medical Microbiology, Institute of Infection and Immunity, Cardiff University, Cardiff, United Kingdom. portale@cardiff.ac.uk. 7. School of Medicine, Department of Medical Microbiology, Institute of Infection and Immunity, Cardiff University, Cardiff, United Kingdom. ian.boostrom@gmail.com. 8. Sheikh Hasina National Institute of Burn and Plastic Surgery, Dhaka, Bangladesh. kalammishu@yahoo.com. 9. School of Medicine, Department of Medical Microbiology, Institute of Infection and Immunity, Cardiff University, Cardiff, United Kingdom. hassanb1@cardiff.ac.uk. 10. Dhaka Medical College Hospital, Dhaka, Bangladesh. dmch@hospi.dghs.gov.bd. 11. School of Medicine, Department of Medical Microbiology, Institute of Infection and Immunity, Cardiff University, Cardiff, United Kingdom. walshtr@cardiff.ac.uk.
Abstract
INTRODUCTION: The emergence of plasmid mediated mcr in bacteria has become global public health threat. Herein, we report a mcr-1 positive E. coli in normal human flora from a patient admitted in Dhaka Medical College Hospital (DMCH). METHODOLOGY: In total, 700 non-duplicate rectal swabs were collected from DMCH during 13th May to 12th June 2018. E. coli from rectal swabs were isolated on chromogenic UTI media containing vancomycin 10mg/l (Liofilchem, Italy) and confirmed by MALDI-TOF. Minimum inhibitory concentrations (MIC) were determined by agar dilution and interpreted according to EUCAST breakpoints. Genomic analysis of mcr positive E. coli (MCRPEC) was performed by Illumina MiSeq sequencing and pulsed field gel electrophoresis (PFGE) using S1 nuclease DNA digests and blamcr-1 probing. Transferability of blamcr-1 were determined by conjugation assays. RESULTS: We found one MCRPEC from 700 rectal swab screening which was isolated from the rectal swab culture of a 17-year boy who was admitted to the burns ICU, DMCH with 53% flame burn involving much of the trunk and face. Genome sequencing revealed that mcr-1 was present on an IncH12 plasmid of 257,243 bp and flanked by ISApaI1. The colistin resistance can be transferred to the recipient Klebsiella varricola with a frequency of 8.3 × 10-5. Transconjugants were more resistant to colistin than donor (MIC 32 µg/mL). CONCLUSIONS: This is the first human associated mcr in Bangladesh. These data indicate the need for a systematic "one health" surveillance in the country. Copyright (c) 2019 Refath Farzana, Lim S Jones, Md. Anisur Rahman, Mark A Toleman, Kirsty Sands, Edward Portal, Ian Boostrom, Md. Abul Kalam, Brekhna Hassan, A K M Nasir Uddin, Timothy R Walsh.
INTRODUCTION: The emergence of plasmid mediated mcr in bacteria has become global public health threat. Herein, we report a mcr-1 positive E. coli in normal human flora from a patient admitted in Dhaka Medical College Hospital (DMCH). METHODOLOGY: In total, 700 non-duplicate rectal swabs were collected from DMCH during 13th May to 12th June 2018. E. coli from rectal swabs were isolated on chromogenic UTI media containing vancomycin 10mg/l (Liofilchem, Italy) and confirmed by MALDI-TOF. Minimum inhibitory concentrations (MIC) were determined by agar dilution and interpreted according to EUCAST breakpoints. Genomic analysis of mcr positive E. coli (MCRPEC) was performed by Illumina MiSeq sequencing and pulsed field gel electrophoresis (PFGE) using S1 nuclease DNA digests and blamcr-1 probing. Transferability of blamcr-1 were determined by conjugation assays. RESULTS: We found one MCRPEC from 700 rectal swab screening which was isolated from the rectal swab culture of a 17-year boy who was admitted to the burns ICU, DMCH with 53% flame burn involving much of the trunk and face. Genome sequencing revealed that mcr-1 was present on an IncH12 plasmid of 257,243 bp and flanked by ISApaI1. The colistin resistance can be transferred to the recipient Klebsiella varricola with a frequency of 8.3 × 10-5. Transconjugants were more resistant to colistin than donor (MIC 32 µg/mL). CONCLUSIONS: This is the first human associated mcr in Bangladesh. These data indicate the need for a systematic "one health" surveillance in the country. Copyright (c) 2019 Refath Farzana, Lim S Jones, Md. Anisur Rahman, Mark A Toleman, Kirsty Sands, Edward Portal, Ian Boostrom, Md. Abul Kalam, Brekhna Hassan, A K M Nasir Uddin, Timothy R Walsh.
Authors: Refath Farzana; Lim S Jones; Andrew Barratt; Muhammad Anisur Rahman; Kirsty Sands; Edward Portal; Ian Boostrom; Laura Espina; Monira Pervin; A K M Nasir Uddin; Timothy R Walsh Journal: mSphere Date: 2020-03-11 Impact factor: 4.389
Authors: Carlos Bastidas-Caldes; Jacobus H de Waard; María Soledad Salgado; María José Villacís; Marco Coral-Almeida; Yoshimasa Yamamoto; Manuel Calvopiña Journal: Pathogens Date: 2022-06-08
Authors: Raquel Girardello; Carlos Morais Piroupo; Joaquim Martins; Marcia Helena Maffucci; Ana Paula Cury; Maria Renata Gomes Franco; Fernanda de Mello Malta; Natália Conceição Rocha; João Renato Rebello Pinho; Flavia Rossi; Alberto José da Silva Duarte; João Carlos Setubal Journal: Front Microbiol Date: 2021-06-09 Impact factor: 5.640