Ahmet Çalışkan1, Ayşegül Çopur Çicek2, Nebahat Aydogan Ejder3, Alper Karagöz4, Özlem Kirişci5, Selçuk Kılıç6. 1. Department of Medical Microbiology, Faculty of Medicine, Pamukkale University, Denizli, Turkey. ahmetsuna@msn.com. 2. Department of Medical Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey. acopurcicek@gmail.com. 3. Department of Medical Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey. nebahat.aydogan@erdogan.edu.tr. 4. Department of Microbiology, Molecular Biology and Genetic, Uşak University, Uşak, Turkey. alper.karagoz82@gmail.com. 5. Microbiology Laboratory, Necip Fazıl City Hospital, Kahramanmaraş, Turkey. dr_ozlemgitmisoglu@hotmail.com. 6. Microbiology Reference Laboratories Department, Public Health Institutes of Turkey, Ankara, Turkey. selcuk.kilic@rshm.gov.tr.
Abstract
INTRODUCTION: Stenotrophomonas maltophilia, which is able to form a biofilm, has mostly been related to catheters when it is the agent in hospital infections; these infections generally present as bacteremia and pneumonia, which may progress with complications and result in death. METHODOLOGY: The study included 153 S. maltophilia strains isolated from clinical samples sent to our hospital laboratory between 1 January 2014 and 30 June 2018. The bacteria were identified and their antibiotic sensitivity was determined using the VITEK-2 automated system. PFGE (Pulsed Field Gel Electrophoresis): The strains isolated from 34 patient clinical samples and from 1 patient bedcover were taken for PFGE examination. RESULTS: The TMP/SXT and levofloxacin sensitivity of 153 S. maltophilia strains was examined. TMP/SXT resistance was determined to be 39% and levofloxacin resistance at 5%. Among 35 S. maltophilia strains, seven genotypes were identified using the PFGE method. While three strains showed a specific genotype profile, the other 32 were determined to consist of four clusters. The cluster rate was therefore 91.4% (32/35). CONCLUSIONS: There was a clonal relationship between the vast majority of the 35 S. maltophilia isolates, which suggests that there was a cross-contamination problem in the hospital. One strain (#4) was identified by dendrogram analysis showed a high rate of similarity to the other strains and was determined to be the common source of the cross-contamination. Copyright (c) 2019 Ahemr Caliksan, Aysegul Copur Cicek, Nebahat Aydogan Ejder, Alper Karagoz, Ozlem Kirisci, Selcuk Kilic.
INTRODUCTION:Stenotrophomonas maltophilia, which is able to form a biofilm, has mostly been related to catheters when it is the agent in hospital infections; these infections generally present as bacteremia and pneumonia, which may progress with complications and result in death. METHODOLOGY: The study included 153 S. maltophilia strains isolated from clinical samples sent to our hospital laboratory between 1 January 2014 and 30 June 2018. The bacteria were identified and their antibiotic sensitivity was determined using the VITEK-2 automated system. PFGE (Pulsed Field Gel Electrophoresis): The strains isolated from 34 patientclinical samples and from 1 patient bedcover were taken for PFGE examination. RESULTS: The TMP/SXT and levofloxacin sensitivity of 153 S. maltophilia strains was examined. TMP/SXT resistance was determined to be 39% and levofloxacin resistance at 5%. Among 35 S. maltophilia strains, seven genotypes were identified using the PFGE method. While three strains showed a specific genotype profile, the other 32 were determined to consist of four clusters. The cluster rate was therefore 91.4% (32/35). CONCLUSIONS: There was a clonal relationship between the vast majority of the 35 S. maltophilia isolates, which suggests that there was a cross-contamination problem in the hospital. One strain (#4) was identified by dendrogram analysis showed a high rate of similarity to the other strains and was determined to be the common source of the cross-contamination. Copyright (c) 2019 Ahemr Caliksan, Aysegul Copur Cicek, Nebahat Aydogan Ejder, Alper Karagoz, Ozlem Kirisci, Selcuk Kilic.
Entities:
Keywords:
PFGE; Pulsed Field Gel Electrophoresis; antibiotic sensitivity; clonal outbreak