Pénélope Bourgoin1,2, Guillaume Lediagon3, Isabelle Arnoux4, Denis Bernot4, Pierre-Emmanuel Morange2,4, Pierre Michelet3, Fabrice Malergue1, Thibaut Markarian3. 1. Department of Research & Development, Beckman Coulter Life Sciences-Immunotech, 130 Avenue de Lattre de Tassigny, 13009 Marseille, France. 2. C2VN INSERM-INRA, Aix-Marseille University, 27 Boulevard Jean Moulin, 13385 Marseille, France. 3. Adult Emergency Unit, La Timone Hospital, APHM, 264 Rue Saint Pierre, 13385 Marseille, France. 4. Hematology Laboratory, La Timone Hospital, APHM, 264 Rue Saint Pierre, 13385 Marseille, France.
Abstract
Aim: In an Emergency Department (ED), the etiological identification of infected subjects is essential. 13 infection-related biomarkers were assessed using a new flow cytometry procedure. Materials & methods: If subjects presented with febrile symptoms at the ED, 13 biomarkers' levels, including CD64 on neutrophils (nCD64) and CD169 on monocytes (mCD169), were tested and compared with clinical records. Results: Among 50 subjects, 78% had bacterial infections and 8% had viral infections. nCD64 showed 82% sensitivity and 91% specificity for identifying subjects with bacterial infections. mCD169, HLA-ABC ratio and HLA-DR on monocytes had high values in subjects with viral infections. Conclusion: Biomarkers showed promising performances to improve the ED's infectious stratification.
Aim: In an Emergency Department (ED), the etiological identification of infected subjects is essential. 13 infection-related biomarkers were assessed using a new flow cytometry procedure. Materials & methods: If subjects presented with febrile symptoms at the ED, 13 biomarkers' levels, including CD64 on neutrophils (nCD64) and CD169 on monocytes (mCD169), were tested and compared with clinical records. Results: Among 50 subjects, 78% had bacterial infections and 8% had viral infections. nCD64 showed 82% sensitivity and 91% specificity for identifying subjects with bacterial infections. mCD169, HLA-ABC ratio and HLA-DR on monocytes had high values in subjects with viral infections. Conclusion: Biomarkers showed promising performances to improve the ED's infectious stratification.
Authors: Alejandra Comins-Boo; Maria Gutiérrez-Larrañaga; Adriel Roa-Bautista; Sandra Guiral Foz; Mónica Renuncio García; Elena González López; Juan Irure Ventura; María Carmen Fariñas-Álvarez; David San Segundo; Marcos López Hoyos Journal: Front Med (Lausanne) Date: 2021-03-23
Authors: Pénélope Bourgoin; Thomas Soliveres; Alexandra Barbaresi; Anderson Loundou; Inès Ait Belkacem; Isabelle Arnoux; Denis Bernot; Marie Loosveld; Pierre-Emmanuel Morange; Pierre Michelet; Fabrice Malergue; Thibaut Markarian Journal: Cytometry A Date: 2021-02-08 Impact factor: 4.714