n-3 polyunsaturated fatty acids as modulators of thermogenesis in Ames dwarf mice.

Lipids, commonly split into saturated and mono- and polyunsaturated fatty acids, are key constituents of all biological membranes, and their exact proportions in different tissues were previously shown to be related to lifespan in mammals. As a mechanism, it was put forward that long-chain and highly unsaturated n-3 fatty acids may act as "pacemakers" in membranes while the n-6 fatty acid class may act as a counterbalance. Previously, long-lived Ames dwarf mice (Prop1 df/df) were found to have lower n-3 fatty acids and higher n-6 throughout their tissues. We exposed 32 adult (8 months old) Ames dwarf mice to three isocaloric diets differing in their fatty acid composition (saturated vs. rich in n-3 and n-6) for 2 months while measuring their body masses, subcutaneous body temperatures and finally membrane fatty acid profiles. Prominently, we found that individuals from all three groups quickly increased their body masses by ca. 20% and had 0.45 °C higher subcutaneous body temperatures than at baseline (F1,12,16 = 22.27; p < 0.001). Conceivably, experimental diets also largely reflected lipid composition found in the tissues with over 50% n-3 fatty acids in heart phospholipids from animals from the n-3-enriched feeding group. Our study indicates that fatty acid-enriched diets well affected body mass, subcutaneous body temperature and membrane fatty acid composition in Ames dwarf mice with no visible adverse effects on their health. Experimental feeding increased subcutaneous body fat and insulation, most likely explaining the higher subcutaneous temperatures.