Mehmet Ozansoy1, Muzaffer Beyza Ozansoy2, Burak Yulug3, Seyda Cankaya4, Ertugrul Kilic5, Sule Goktekin6, Ulkan Kilic7. 1. T.C. Istanbul Bahcesehir University, School of Medicine, Dept. of Physiology, Istanbul, Turkey; T.C. Istanbul Medipol University, Regenerative and Restorative Medical Research Center (REMER), Istanbul, Turkey. 2. T.C. Istanbul Aydin University, School of Medicine, Dept. of Physiology, Istanbul, Turkey; T.C. Istanbul Medipol University, Regenerative and Restorative Medical Research Center (REMER), Istanbul, Turkey. 3. Alaaddin Keykubat University, The Faculty of Medicine, Dept. of Neurology, Alanya, Turkey; T.C. Istanbul Medipol University, Regenerative and Restorative Medical Research Center (REMER), Istanbul, Turkey. 4. Alaaddin Keykubat University, The Faculty of Medicine, Dept. of Neurology, Alanya, Turkey. 5. T.C. Istanbul Medipol University, School of Medicine, Dept. of Physiology, Istanbul, Turkey; T.C. Istanbul Medipol University, Regenerative and Restorative Medical Research Center (REMER), Istanbul, Turkey. 6. T.C. Istanbul Medipol University, Regenerative and Restorative Medical Research Center (REMER), Istanbul, Turkey. 7. University of Health Sciences, Medical School, Department of Medical Biology, Istanbul, Turkey. Electronic address: ulkan.kilic@sbu.edu.tr.
Abstract
BACKGROUND: Recent studies have been revealed that oxidative damage is the main cause of aging and age-related neurodegenerative diseases like Alzheimer's disease (AD). Melatonin is secreted from the pineal gland and its secretion has been found to be altered in AD. In the last decade the role of exosomes in spreading toxic proteins and inducing the propagation of diseases like AD has been discussed. However, it is not known how melatonin affects the amount of exosomes released from the cells and the content of the exosomes. OBJECTIVE: Herein, we investigated the possible role of melatonin treatment in the releasing of exosomes and exosomal tau content in an in vitro Aβ toxicity model. METHOD: SH-SY5Y cell line was used. The optimum concentration of Aβ was determined by cell viability and cell proliferation tests. Melatonin (100 µM) was applied before and after Aβ application. Total exosomes isolated from cell culture media were immunoprecipitated. The amount of released exosomes and their tau content were analyzed by Western blots. RESULTS: Our data demonstrated for the first time that melatonin treatment clearly affected the amount of released exosomes. It would decrease the amyloid beta load and toxicity by inhibiting exosome release. We also demonstated that melatonin also affected the level of tau carried by exosomes depending on whether melatonin was applied before or after Aβ application. CONCLUSION: It is considered that the effect of melatonin in the release of exosomes and exosomal tau content would contribute the development of therapeutic strategies in AD and related disorders.
BACKGROUND: Recent studies have been revealed that oxidative damage is the main cause of aging and age-related neurodegenerative diseases like Alzheimer's disease (AD). Melatonin is secreted from the pineal gland and its secretion has been found to be altered in AD. In the last decade the role of exosomes in spreading toxic proteins and inducing the propagation of diseases like AD has been discussed. However, it is not known how melatonin affects the amount of exosomes released from the cells and the content of the exosomes. OBJECTIVE: Herein, we investigated the possible role of melatonin treatment in the releasing of exosomes and exosomal tau content in an in vitro Aβ toxicity model. METHOD:SH-SY5Y cell line was used. The optimum concentration of Aβ was determined by cell viability and cell proliferation tests. Melatonin (100 µM) was applied before and after Aβ application. Total exosomes isolated from cell culture media were immunoprecipitated. The amount of released exosomes and their tau content were analyzed by Western blots. RESULTS: Our data demonstrated for the first time that melatonin treatment clearly affected the amount of released exosomes. It would decrease the amyloid beta load and toxicity by inhibiting exosome release. We also demonstated that melatonin also affected the level of tau carried by exosomes depending on whether melatonin was applied before or after Aβ application. CONCLUSION: It is considered that the effect of melatonin in the release of exosomes and exosomal tau content would contribute the development of therapeutic strategies in AD and related disorders.