Murilo de Oliveira Brito1, Meliza Arantes de Souza Bessa2, Ralciane de Paula Menezes3, Denise Von Dolinger de Brito Röder4, Mário Paulo Amante Penatti5, João Paulo Pimenta6, Paula Augusta Dias Fogaça de Aguiar7, Reginaldo Dos Santos Pedroso8. 1. Faculty of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil. britomurilo51@gmail.com. 2. Institute of Biology, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil. melizaarantes@gmail.com. 3. Faculty of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil. ralciane@ufu.br. 4. Faculty of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil. denise.roder@ufu.br. 5. Technical School of Health, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil. mario.penatti@ufu.br. 6. Checkup Medical Laboratory, Uberlandia, Minas Gerais, Brazil. joao.paulopimenta490@gmail.com. 7. Faculty of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil. paulinhapatoclinica@bol.com.br. 8. Faculty of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil. rpedroso@ufu.br.
Abstract
INTRODUCTION: Fungi of the genus Cryptococcus are cosmopolitan and may be agents of opportunistic mycoses in immunocompromised and sometimes immunocompetent individuals. Cryptococcus species are frequently isolated from trees and bird excreta in the environment and infection occurs by inhalation of propagules dispersed in the air. The aim was to investigate Cryptococcus species in bird excreta and tree hollows located in a university hospital area and in an academic area of a university campus. METHODOLOGY: A total of 40 samples of bird excreta and 41 samples of tree hollows were collected. The identification of the isolates was done by classical methodology and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. RESULTS: Twenty (62.5%) isolates of Cryptococcus were found in bird excreta and 12 (37.5%) in tree hollows. C. laurentii (currently Papiliotrema laurentii) was the most frequent species in both samples, being found in 5 samples of excreta and in 8 tree hollows. The diversity of species found in excreta (C. laurentii, C. albidus [currently Naganishia albida], C. liquefaciens [currently N. liquefaciens], C. friedmanii [currently N. friedmannii] and others) was higher than in tree hollows (C. laurentii, C. flavescens [currently Papiliotrema flavescens], and other yeasts). CONCLUSION: Many Cryptococcus species were isolated from excreta and tree hollows, and this fact is important for understanding the environmental epidemiology of those emerging pathogens for public health, as a way to implement surveillance actions and control of cryptococcosis. Copyright (c) 2019 Murilo de Oliveira Brito, Meliza Arantes de Souza Bessa, Ralciane de Paula Menezes, Denise Von Dolinger de Brito Roder, Mario Paulo Amante Penatti, Joao Paulo Pimenta, Paula Augusta Dias Fogaca de Aguiar, Reginaldo dos Santos Pedroso.
INTRODUCTION: Fungi of the genus Cryptococcus are cosmopolitan and may be agents of opportunistic mycoses in immunocompromised and sometimes immunocompetent individuals. Cryptococcus species are frequently isolated from trees and bird excreta in the environment and infection occurs by inhalation of propagules dispersed in the air. The aim was to investigate Cryptococcus species in bird excreta and tree hollows located in a university hospital area and in an academic area of a university campus. METHODOLOGY: A total of 40 samples of bird excreta and 41 samples of tree hollows were collected. The identification of the isolates was done by classical methodology and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. RESULTS: Twenty (62.5%) isolates of Cryptococcus were found in bird excreta and 12 (37.5%) in tree hollows. C. laurentii (currently Papiliotrema laurentii) was the most frequent species in both samples, being found in 5 samples of excreta and in 8 tree hollows. The diversity of species found in excreta (C. laurentii, C. albidus [currently Naganishia albida], C. liquefaciens [currently N. liquefaciens], C. friedmanii [currently N. friedmannii] and others) was higher than in tree hollows (C. laurentii, C. flavescens [currently Papiliotrema flavescens], and other yeasts). CONCLUSION: Many Cryptococcus species were isolated from excreta and tree hollows, and this fact is important for understanding the environmental epidemiology of those emerging pathogens for public health, as a way to implement surveillance actions and control of cryptococcosis. Copyright (c) 2019 Murilo de Oliveira Brito, Meliza Arantes de Souza Bessa, Ralciane de Paula Menezes, Denise Von Dolinger de Brito Roder, Mario Paulo Amante Penatti, Joao Paulo Pimenta, Paula Augusta Dias Fogaca de Aguiar, Reginaldo dos Santos Pedroso.
Authors: Lana Sarita de Souza Oliveira; Luciana Magalhães Pinto; Mariana Araújo Paulo de Medeiros; Dena L Toffaletti; Jennifer L Tenor; Tânia Fraga Barros; Rejane Pereira Neves; Reginaldo Gonçalves de Lima Neto; Eveline Pipolo Milan; Ana Carolina Barbosa Padovan; Walicyranison Plinio da Silva Rocha; John R Perfect; Guilherme Maranhão Chaves Journal: Front Cell Infect Microbiol Date: 2021-07-01 Impact factor: 5.293