Ulises Hernández-Chiñas1, Alejandro Pérez-Ramos2, Laura Belmont-Monroy3, María E Chávez-Berrocal4, Edgar González-Villalobos5, Armando Navarro-Ocaña6, Carlos A Eslava7, Jose Molina-Lopez8. 1. Unidad Periférica de Investigación Básica y Clínica en Enfermedades Infecciosas; Departamento de Salud Pública/División de Investigación, Facultad de Medicina, UNAM, Ciudad de México, México. ulisesh@unam.mx. 2. Unidad Periférica de Investigación Básica y Clínica en Enfermedades Infecciosas; Departamento de Salud Pública/División de Investigación, Facultad de Medicina, UNAM, Ciudad de México, México. uliseshch@hotmail.com. 3. Unidad Periférica de Investigación Básica y Clínica en Enfermedades Infecciosas; Departamento de Salud Pública/División de Investigación, Facultad de Medicina, UNAM, Ciudad de México, México. arual_ch19@yahoo.com.mx. 4. Unidad Periférica de Investigación Básica y Clínica en Enfermedades Infecciosas; Departamento de Salud Pública/División de Investigación, Facultad de Medicina, UNAM, Ciudad de México, México. malenachavezb@yahoo.com.mx. 5. Unidad Periférica de Investigación Básica y Clínica en Enfermedades Infecciosas; Departamento de Salud Pública/División de Investigación, Facultad de Medicina, UNAM, Ciudad de México, México. qbp_villalobos@hotmail.com. 6. Laboratorio de Bacteriología, Departamento de Salud Pública, Facultad de Medicina, Ciudad de México, México. arnava@unam.mx. 7. Unidad Periférica de Investigación Básica y Clínica en Enfermedades Infecciosas; Departamento de Salud Pública/División de Investigación, Facultad de Medicina, UNAM, Ciudad de México, México. eslava@unam.mx. 8. Unidad Periférica de Investigación Básica y Clínica en Enfermedades Infecciosas; Departamento de Salud Pública/División de Investigación, Facultad de Medicina, UNAM, Ciudad de México, México. joseml@unam.mx.
Abstract
INTRODUCTION: Uropathogenic Escherichia coli (UPEC) are the main etiological agent of urinary tract infections (UTIs). Association between different serotypes and UTIs is known, however, some strains are incapable to be serotyped. The aim of this work was to study bthe phenotypical and genotypical characteristics of 113 non-typeable (NT) and auto-agglutinating (AA) E. coli strains, isolated from UTIs in children and adults. METHODOLOGY: The 113 UPEC strains were analyzed by PCR assays using specific primers to determine their serogroups, fimH, papC, iutA, sat, hlyCA and cnf1, virulence associated genes, and chuA, yjaA and TSPE4.C2 for phylogroup determination. Additionally, the diffusion disk method was performed to evaluate the antimicrobial resistance to 18 antimicrobial agents. RESULTS: Using the PCR assay, 63% (71) of the strains were genotyped showing O25 and O75 as the most common serogroups. The virulence genes fimH (86%) and iutA (74%) were the most prevalent, in relation to the phylogroups the commensal (A and B1) and virulent (B2 and D) showed similar frequencies (P > 0.05). The antimicrobial susceptibility test showed a high percentage (73%) of multidrug-resistant strains. CONCLUSIONS: The genotyping allowed identifying the serogroup in many of the strains that could not be typed by traditional serology. The strains carried virulence genes and were multidrug-resistant in both, commensal and virulent phylogroups. Our findings revealed that, in addition to the classical UPEC serogroups, there are pathogenic serogroups not reported yet. Copyright (c) 2019 Molina Lopez Jose, Ulises Hernandez-Chinas, Alejandro Perez-Ramos, Laura Belmont-Monroy, Maria E Chavez-Berrocal, Edgar Gonzalez-Villalobos, Armando Navarro-Ocana, Carlos A Eslava.
INTRODUCTION: Uropathogenic Escherichia coli (UPEC) are the main etiological agent of urinary tract infections (UTIs). Association between different serotypes and UTIs is known, however, some strains are incapable to be serotyped. The aim of this work was to study bthe phenotypical and genotypical characteristics of 113 non-typeable (NT) and auto-agglutinating (AA) E. coli strains, isolated from UTIs in children and adults. METHODOLOGY: The 113 UPEC strains were analyzed by PCR assays using specific primers to determine their serogroups, fimH, papC, iutA, sat, hlyCA and cnf1, virulence associated genes, and chuA, yjaA and TSPE4.C2 for phylogroup determination. Additionally, the diffusion disk method was performed to evaluate the antimicrobial resistance to 18 antimicrobial agents. RESULTS: Using the PCR assay, 63% (71) of the strains were genotyped showing O25 and O75 as the most common serogroups. The virulence genes fimH (86%) and iutA (74%) were the most prevalent, in relation to the phylogroups the commensal (A and B1) and virulent (B2 and D) showed similar frequencies (P > 0.05). The antimicrobial susceptibility test showed a high percentage (73%) of multidrug-resistant strains. CONCLUSIONS: The genotyping allowed identifying the serogroup in many of the strains that could not be typed by traditional serology. The strains carried virulence genes and were multidrug-resistant in both, commensal and virulent phylogroups. Our findings revealed that, in addition to the classical UPEC serogroups, there are pathogenic serogroups not reported yet. Copyright (c) 2019 Molina Lopez Jose, Ulises Hernandez-Chinas, Alejandro Perez-Ramos, Laura Belmont-Monroy, Maria E Chavez-Berrocal, Edgar Gonzalez-Villalobos, Armando Navarro-Ocana, Carlos A Eslava.
Entities:
Keywords:
E. coli auto-agglutinating; E. coli non-serotypeable; Urinary tract infections; serogroup; uropathogenic Escherichia coli
Authors: Edgar González-Villalobos; Rosa María Ribas-Aparicio; Gerardo Erbey Rodea Montealegre; Laura Belmont-Monroy; Yerisaidy Ortega-García; Gerardo Aparicio-Ozores; José Luis Balcázar; Carlos Alberto Eslava-Campos; Ulises Hernández-Chiñas; José Molina-López Journal: Appl Microbiol Biotechnol Date: 2021-07-12 Impact factor: 4.813