Silicon and plant growth promoting rhizobacteria differentially regulate AgNP-induced toxicity in Brassica juncea: Implication of nitric oxide.

An emerging stress of nanomaterials in soil and water is of great concern as it limits crop productivity and affects humans as well. Therefore, it is required to manage this problem. Silicon and plant growth promoting rhizobacteria has gained the engaging role in agriculture as (bio-)fertilizers. However, their role against silver nanoparticles (AgNPs) is still not known. Hence, present study was envisaged to investigate role of Si, PGPR and phytohormone indole acetic acid (IAA) in regulating AgNP stress in Brassica juncea seedlings. The study highlighted the impact of various treatments with respect to overproduction of reactive oxygen species, signaling molecule nitric oxide, oxidative markers like antioxidant enzymes and nonenzymatic components of ascorbate-glutathione pathway. Interestingly, silicon when present with AgNPs enhanced toxicity by reducing growth and mechanistic properties of B. juncea. Moreover, the results highlight the role of PGPR and IAA towards reduction in toxicity by promoting the plant growth under stressed conditions. Treatments AgNP + Si + PGPR/IAA were observed to significantly reduce the stress and enhance plant growth against treatment AgNPs alone. This reversal in toxicity by PGPR and IAA along with Si suggests the idea to formulate and utilize their combination as biofertilizers for eradicating the stress in near future.