Zhengbing Wang1, Chuanhe Gao1, Jiali Liu1, Wenwu Zhou2, Xinnian Zeng1. 1. Guangdong Engineering Research Center for Insect Behavior Regulation; Key Laboratory of Bio-Pesticide Innovation and Application, College of Agriculture, South China Agricultural University, Guangzhou, China. 2. State Key Laboratory of Rice Biology; Ministry of Agriculture, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Abstract
BACKGROUND: The Asian citrus psyllid (ACP), Diaphorina citri, is one of the major pests in citrus production because it transmits huanglongbing, a devastating disease of citrus plants. Odourant-binding proteins (OBPs) play an important role in the olfactory perception of insects. Revealing the function of DcitOBPs is beneficial to the development of new ACP management strategies. RESULTS: An analysis of the components of volatiles from the new shoots of six host plant species showed that β-caryophyllene was the characteristic volatile compound in flushing shoots and the most abundant volatile compound in three of the six tested ACP host plant species. The tissue expression profiles of nine known DcitOBPs were analyzed based on a transcriptome database, and DcitOBP3 and DcitOBP6 exhibited high expression in the antennae of both sexes and the ovipositor of females. The binding ability of two recombinant proteins with eight ligands was studied through competitive binding analysis; the results showed that DcitOBP6 exhibited stronger binding to β-caryophyllene. Behavioural trials indicated that sexually mature female adults of D. citri were significantly attracted to β-caryophyllene at concentrations of 0.1 μL mL-1 and 10 μL mL-1 . RNAi analysis in female D. citri showed that the reduction of DcitOBP6 transcript abundance led to a decrease in antennae EAG activity and behavioural responses to β-caryophyllene. CONCLUSION: The results demonstrate that DcitOBP6 is involved in the perception of an important host plant volatile, β-caryophyllene, in the ACP, and provide a theoretical foundation for behavioural interference in ACP management.
BACKGROUND: The Asian citrus psyllid (ACP), Diaphorina citri, is one of the major pests in citrus production because it transmits huanglongbing, a devastating disease of citrus plants. Odourant-binding proteins (OBPs) play an important role in the olfactory perception of insects. Revealing the function of DcitOBPs is beneficial to the development of new ACP management strategies. RESULTS: An analysis of the components of volatiles from the new shoots of six host plant species showed that β-caryophyllene was the characteristic volatile compound in flushing shoots and the most abundant volatile compound in three of the six tested ACP host plant species. The tissue expression profiles of nine known DcitOBPs were analyzed based on a transcriptome database, and DcitOBP3 and DcitOBP6 exhibited high expression in the antennae of both sexes and the ovipositor of females. The binding ability of two recombinant proteins with eight ligands was studied through competitive binding analysis; the results showed that DcitOBP6 exhibited stronger binding to β-caryophyllene. Behavioural trials indicated that sexually mature female adults of D. citri were significantly attracted to β-caryophyllene at concentrations of 0.1 μL mL-1 and 10 μL mL-1 . RNAi analysis in female D. citri showed that the reduction of DcitOBP6 transcript abundance led to a decrease in antennae EAG activity and behavioural responses to β-caryophyllene. CONCLUSION: The results demonstrate that DcitOBP6 is involved in the perception of an important host plant volatile, β-caryophyllene, in the ACP, and provide a theoretical foundation for behavioural interference in ACP management.