Carla Fontana1,2, Silvia Angeletti3, Walter Mirandola1, Eleonora Cella4, Lai Alessia5, Gianguglielmo Zehender5, Marco Favaro2, Davide Leoni6, Diego Delle Rose6, Giovanni Gherardi3, Lucia De Florio3, Marco Salemi7,8, Massimo Andreoni6, Loredana Sarmati6, Massimo Ciccozzi4. 1. Clinical Microbiology Laboratories, Polyclinic of Tor Vergata, Rome, Italy. 2. Department of Experimental Medicine & Biochemical Sciences, University of Rome "Tor Vergata", Rome, Italy. 3. Unit of Clinical Laboratory Science, University Campus Bio-Medico of Rome, Rome, Italy. 4. Unit of Medical Statistics & Molecular Epidemiology, University Campus Bio-Medico of Rome, Italy. 5. Department of Biomedical & Clinical Sciences "L. Sacco", University of Milan, Milan, Italy. 6. Clinical of Infectious Diseases, Department of System Medicine, Tor Vergata University, Rome, Italy. 7. Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA. 8. Department of Pathology, Immunology & Laboratory Medicine, University of Florida, Gainesville, FL 32610, USA.
Abstract
Aim: Carbapenemase-resistant Enterobacteriaceae represents a major concern in hospital setting. Materials & methods: The evolutionary history of carbapenem-resistant Klebsiella pneumonia strains was analyzed by core genome multilocus sequence typing and Bayesian phylogenesis by whole genomes sequencing. Results: A great increase carbapenem-resistant K. pneumoniae causing blood stream infection was observed in the years 2015-2016. At multilocus sequence typing (MLST), they were prevalently ST512 and ST101. ST512 were core genome (cg)MLST 53, while ST101 mainly cgMLST453. The minimum-spanning tree, based on cgMLST, showed strains clustering based on the different STs. By Bayesian phylogenetic analysis, maximum clade credibility tree showed that strains were introduced in the year 2005 with the most probable location in the ICU ward. Two outbreaks by ST101 and ST512 strains with Tower T8 as the probable location were evidenced. Conclusion: Molecular epidemiology is a powerful tool to track the way of transmission of resistant bacteria within the hospital setting.
Aim: Carbapenemase-resistant Enterobacteriaceae represents a major concern in hospital setting. Materials & methods: The evolutionary history of carbapenem-resistant Klebsiella pneumonia strains was analyzed by core genome multilocus sequence typing and Bayesian phylogenesis by whole genomes sequencing. Results: A great increase carbapenem-resistant K. pneumoniae causing blood stream infection was observed in the years 2015-2016. At multilocus sequence typing (MLST), they were prevalently ST512 and ST101. ST512 were core genome (cg)MLST 53, while ST101 mainly cgMLST453. The minimum-spanning tree, based on cgMLST, showed strains clustering based on the different STs. By Bayesian phylogenetic analysis, maximum clade credibility tree showed that strains were introduced in the year 2005 with the most probable location in the ICU ward. Two outbreaks by ST101 and ST512 strains with Tower T8 as the probable location were evidenced. Conclusion: Molecular epidemiology is a powerful tool to track the way of transmission of resistant bacteria within the hospital setting.
Authors: Kalisvar Marimuthu; Indumathi Venkatachalam; Vanessa Koh; Stephan Harbarth; Eli Perencevich; Benjamin Pei Zhi Cherng; Raymond Kok Choon Fong; Surinder Kaur Pada; Say Tat Ooi; Nares Smitasin; Koh Cheng Thoon; Paul Anantharajah Tambyah; Li Yang Hsu; Tse Hsien Koh; Partha Pratim De; Thean Yen Tan; Douglas Chan; Rama Narayana Deepak; Nancy Wen Sim Tee; Andrea Kwa; Yiying Cai; Yik-Ying Teo; Natascha May Thevasagayam; Sai Rama Sridatta Prakki; Weizhen Xu; Wei Xin Khong; David Henderson; Nicole Stoesser; David W Eyre; Derrick Crook; Michelle Ang; Raymond Tzer Pin Lin; Angela Chow; Alex R Cook; Jeanette Teo; Oon Tek Ng Journal: Nat Commun Date: 2022-06-01 Impact factor: 17.694