| Literature DB >> 32054756 |
Timothy Q DuBuc1, Christine E Schnitzler2,3, Eleni Chrysostomou1, Emma T McMahon1, James M Gahan1, Tara Buggie1, Sebastian G Gornik1, Shirley Hanley4, Sofia N Barreira5, Paul Gonzalez5, Andreas D Baxevanis5, Uri Frank6.
Abstract
Clonal animals do not sequester a germ line during embryogenesis. Instead, they have adult stem cells that contribute to somatic tissues or gametes. How germ fate is induced in these animals, and whether this process is related to bilaterian embryonic germline induction, is unknown. We show that transcription factor AP2 (Tfap2), a regulator of mammalian germ lines, acts to commit adult stem cells, known as i-cells, to the germ cell fate in the clonal cnidarian Hydractinia symbiolongicarpus Tfap2 mutants lacked germ cells and gonads. Transplanted wild-type cells rescued gonad development but not germ cell induction in Tfap2 mutants. Forced expression of Tfap2 in i-cells converted them to germ cells. Therefore, Tfap2 is a regulator of germ cell commitment across germ line-sequestering and germ line-nonsequestering animals.Entities:
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Year: 2020 PMID： 32054756 PMCID： PMC7025884 DOI： 10.1126/science.aay6782
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728