Ivana Buha1, Vesna Škodrić-Trifunović2, Tatjana Adžić-Vukičević3, Aleksandra Ilić4, Ana Blanka-Protić5, Mihailo Stjepanovic6, Marina Anđelković7, Miša Vreća8, Jelena Milin-Lazović9, Vesna Spasovski10, Sonja Pavlović11. 1. Clinic of Pulmonology, Clinical Centre of Serbia, Belgrade, Serbia. ivanabuha1@hotmail.com. 2. Clinic of Pulmonology, Clinical Centre of Serbia, Belgrade, Serbia. vesna.skodric@kcs.ac.rs. 3. Clinic of Pulmonology, Clinical Centre of Serbia, Belgrade, Serbia. tatjana.adzic@kcs.ac.rs. 4. Clinic of Pulmonology, Clinical Centre of Serbia, Belgrade, Serbia. sanjadudvarski@yahoo.com. 5. Clinic of Pulmonology, Clinical Centre of Serbia, Belgrade, Serbia. ana.blanka@kcs.ac.rs. 6. Clinic of Pulmonology, Clinical Centre of Serbia, Belgrade, Serbia. mihailostjepanovic@gmail.com. 7. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia. marina.andjelkovic90@gmail.com. 8. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia. sonya@sezampro.rs. 9. School of Medicine, University of Belgrade, Belgrade, Serbia. milinjelena@gmail.com. 10. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia. vekaspasovski@gmail.com. 11. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia. sonja.pavlovic99@gmail.com.
Abstract
INTRODUCTION: Tuberculosis (TBC) is a contagious chronic respiratory disease which despite the known cause, Mycobacterium tuberculosis (Mtb), and many decades of successful therapy, remains one of the leading global health problems. Immune responses against Mtb infection involve both of types of immunity, but cellular immunity, in which certain cytokines and Th1 cells play a key role, is crucial. A better understanding of the functions of the cytokine network involved in the state and progression of TBC could identify specific molecular markers for monitoring of disease activity as well as therapy outcomes in TBC patients. METHODOLOGY: We investigated expression of TNF-α, IL-6 and IRAK1 genes using an RT-qPCR technique in peripheral blood mononuclear cells of 33 TBC patients and 10 healthy individuals. RESULTS: Comparison between TBC patients and healthy individuals revealed statistically significant differences for all analyzed genes. The levels of expression of TNF-α and IL-6 mRNA were higher, while the level of IRAK1 mRNA was lower in the TBC group compared to controls. Moreover, a strong positive correlation was observed between TNF-α and IL-6 gene expression. When clinical parameters were analyzed, increased levels of TNF-α mRNA were detected in patients with a longer duration of therapy (>2 months) compared to those with a shorter therapy duration (< 2 months), and in patients without anemia. CONCLUSIONS: Our results indicate that the inflammatory genes we examined play a crucial role in the pathogenesis of tuberculosis, and that the expression of the TNF-α gene could be a marker for monitoring the clinical effect of the ant-tuberculosis drugs during therapy. Copyright (c) 2019 Ivana Buha, Vesna Skodric-Trifunovic, Tatjana Adzic-Vukicevic, Aleksandra Ilic, Ana Blanka-Protic, Mihailo Stjepanovic, Marina Andelkovic, Misa Vreca, Jelena Milin-Lazovic, Vesna Spasovski, Sonja Pavlovic.
INTRODUCTION:Tuberculosis (TBC) is a contagious chronic respiratory disease which despite the known cause, Mycobacterium tuberculosis (Mtb), and many decades of successful therapy, remains one of the leading global health problems. Immune responses against Mtb infection involve both of types of immunity, but cellular immunity, in which certain cytokines and Th1 cells play a key role, is crucial. A better understanding of the functions of the cytokine network involved in the state and progression of TBC could identify specific molecular markers for monitoring of disease activity as well as therapy outcomes in TBCpatients. METHODOLOGY: We investigated expression of TNF-α, IL-6 and IRAK1 genes using an RT-qPCR technique in peripheral blood mononuclear cells of 33 TBCpatients and 10 healthy individuals. RESULTS: Comparison between TBCpatients and healthy individuals revealed statistically significant differences for all analyzed genes. The levels of expression of TNF-α and IL-6 mRNA were higher, while the level of IRAK1 mRNA was lower in the TBC group compared to controls. Moreover, a strong positive correlation was observed between TNF-α and IL-6 gene expression. When clinical parameters were analyzed, increased levels of TNF-α mRNA were detected in patients with a longer duration of therapy (>2 months) compared to those with a shorter therapy duration (< 2 months), and in patients without anemia. CONCLUSIONS: Our results indicate that the inflammatory genes we examined play a crucial role in the pathogenesis of tuberculosis, and that the expression of the TNF-α gene could be a marker for monitoring the clinical effect of the ant-tuberculosis drugs during therapy. Copyright (c) 2019 Ivana Buha, Vesna Skodric-Trifunovic, Tatjana Adzic-Vukicevic, Aleksandra Ilic, Ana Blanka-Protic, Mihailo Stjepanovic, Marina Andelkovic, Misa Vreca, Jelena Milin-Lazovic, Vesna Spasovski, Sonja Pavlovic.
Authors: Irina Linge; Anastasiya Tsareva; Elena Kondratieva; Alexander Dyatlov; Juan Hidalgo; Ruslan Zvartsev; Alexander Apt Journal: Front Immunol Date: 2022-01-27 Impact factor: 7.561
Authors: Aline de Oliveira Rezende; Rafaella Santos Sabóia; Adeliane Castro da Costa; Diana Messala Pinheiro da Silva Monteiro; Adrielle Zagmignan; Luis Ângelo Macedo Santiago; Rafael Cardoso Carvalho; Paulo Vitor Soeiro Pereira; Ana Paula Junqueira-Kipnis; Eduardo Martins de Sousa Journal: Biomedicines Date: 2022-03-31