Literature DB >> 32053166

Methplotlib: analysis of modified nucleotides from nanopore sequencing.

Wouter De Coster1, Endre Bakken Stovner2,3, Mojca Strazisar1.   

Abstract

SUMMARY: Modified nucleotides play a crucial role in gene expression regulation. Here, we describe methplotlib, a tool developed for the visualization of modified nucleotides detected from Oxford Nanopore Technologies sequencing platforms, together with additional scripts for statistical analysis of allele-specific modification within-subjects and differential modification frequency across subjects.
AVAILABILITY AND IMPLEMENTATION: The methplotlib command-line tool is written in Python3, is compatible with Linux, Mac OS and the MS Windows 10 Subsystem for Linux and released under the MIT license. The source code can be found at https://github.com/wdecoster/methplotlib and can be installed from PyPI and bioconda. Our repository includes test data, and the tool is continuously tested at travis-ci.com. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.
© The Author(s) 2020. Published by Oxford University Press.

Entities:  

Year:  2020        PMID: 32053166     DOI: 10.1093/bioinformatics/btaa093

Source DB:  PubMed          Journal:  Bioinformatics        ISSN: 1367-4803            Impact factor:   6.937


  5 in total

Review 1.  Nanopore sequencing technology, bioinformatics and applications.

Authors:  Yunhao Wang; Yue Zhao; Audrey Bollas; Yuru Wang; Kin Fai Au
Journal:  Nat Biotechnol       Date:  2021-11-08       Impact factor: 54.908

2.  Quantitative profiling of pseudouridylation dynamics in native RNAs with nanopore sequencing.

Authors:  Oguzhan Begik; Morghan C Lucas; Leszek P Pryszcz; Jose Miguel Ramirez; Rebeca Medina; Ivan Milenkovic; Sonia Cruciani; Huanle Liu; Helaine Graziele Santos Vieira; Aldema Sas-Chen; John S Mattick; Schraga Schwartz; Eva Maria Novoa
Journal:  Nat Biotechnol       Date:  2021-05-13       Impact factor: 54.908

3.  Rapid Characterization of Complex Killer Cell Immunoglobulin-Like Receptor (KIR) Regions Using Cas9 Enrichment and Nanopore Sequencing.

Authors:  Jesse Bruijnesteijn; Marit van der Wiel; Natasja G de Groot; Ronald E Bontrop
Journal:  Front Immunol       Date:  2021-09-14       Impact factor: 7.561

4.  Cas9-targeted nanopore sequencing reveals epigenetic heterogeneity after de novo assembly of native full-length hepatitis B virus genomes.

Authors:  Chloe Goldsmith; Damien Cohen; Anaëlle Dubois; Maria Guadalupe Martinez; Kilian Petitjean; Anne Corlu; Barbara Testoni; Hector Hernandez-Vargas; Isabelle Chemin
Journal:  Microb Genom       Date:  2021-05

5.  NanoMethViz: An R/Bioconductor package for visualizing long-read methylation data.

Authors:  Shian Su; Quentin Gouil; Marnie E Blewitt; Dianne Cook; Peter F Hickey; Matthew E Ritchie
Journal:  PLoS Comput Biol       Date:  2021-10-25       Impact factor: 4.475

  5 in total

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