| Literature DB >> 32052888 |
Xueqin Li1,2, Yingying Zhang1,3, Weiya Pei1,2, Mengying Zhang1,2, Hui Yang1,2, Min Zhong1,2, Xiang Kong1,2, Yang Xu1,2, Xiaolong Zhu1,2, Tianbing Chen1,2, Jingjing Ye1,2, Kun Lv1,2.
Abstract
Long non-coding RNAs (lncRNAs) play key roles in various biological processes. However, the roles of lncRNAs in macrophage polarization remain largely unexplored. In this study, thousands of lncRNAs were identified that are differentially expressed in distinct polarized bone marrow-derived macrophages. Among them, Dnmt3aos (DNA methyltransferase 3A, opposite strand), as a known lncRNA, locates on the antisense strand of Dnmt3a. Functional experiments further confirmed that Dnmt3aos were highly expressed in M(IL-4) macrophages and participated in the regulation of Dnmt3a expression, and played a key role in macrophage polarization. The DNA methylation profiles between the Dnmt3aos knockdown group and the control group in M(IL-4) macrophages were determined by MeDIP-seq technique for the first time, and the Dnmt3aos-Dnmt3a axis-mediated DNA methylation modification-regulated macrophage polarization- related gene IFN-γ was identified. Our study will help to enrich our knowledge of the mechanism of macrophage polarization.Entities:
Keywords: DNA methylation; Dnmt3a; LncRNA; macrophage polarization
Year: 2020 PMID： 32052888 DOI： 10.1096/fj.201902379R
Source DB: PubMed Journal: FASEB J ISSN： 0892-6638 Impact factor: 5.191