MiR-34b-5p inhibits cell proliferation, migration and invasion through targeting ARHGAP1 in breast cancer.

This study aim at investigating the function of microRNA (miR)-34b-5p in breast cancer prognosis and development. qRT-PCR was used for miR-34b-5p expression examination in breast cancer samples. CCK8, immunohistochemistry, scratch wound healing, transwell assays were performed for cell experiments. Subcutaneously implanted tumor model was carried out for animal experiment. Western blot was conducted for protein expression detection. Bioinformatics analysis was performed for exploring the underlying mechanism. MiR-34b-5p expression was down-regulated in breast cancer samples and cells, and miR-34d-5p could inhibit cell viability, migration and invasion also delay tumor growth in vivo. Low miR-34b-5p expression showed a bad prognosis of breast cancer patients. MiR-34b-5p functioned as a tumor suppressor by targeting ARHGAP1, and ARHGAP1 knockdown could reverse the effect of miR-34b-5p inhibitor on breast cancer cells. MiR-34b-5p exerts an anti-tumorigenesis role in breast cancer cells by targeting ARHGAP1, which is profitable for the breast cancer diagnosis and molecular treatment. AJTR