Eduard Kabasakalyan1, Karapet Davtyan2, Viktorya Cholakyans3, Alvard Mirzoyan4, Karine Kentenyants5, Diana Petrosyan6, Armen Hayrapetyan7, Himanshu A Gupte8. 1. National Tuberculosis Control Center, SNPO, MoH, Armenia. eduard.kabasakalyan@gmail.com. 2. FMD K and L Europe Contract Research Organization, Yerevan, Armenia. davkaro@gmail.com. 3. National Tuberculosis Control Center, SNPO, MoH, Armenia. cholakyansv@yahoo.com. 4. National Tuberculosis Control Center, SNPO, MoH, Armenia. alvard47@yandex.ru. 5. FMD K and L Europe Contract Research Organization, Yerevan, Armenia. karine.kentenyants@gmail.com. 6. UC Davis Health, Alzheimer 's disease Center, Walnut Creek, California, United States. diana.petrosyan@gmail.com. 7. National Tuberculosis Control Center, SNPO, MoH, Armenia. hayrarmen@gmail.com. 8. Narotam Sekhsaria Foundation, Mumbai, India. himanshu.gupte@gmail.com.
Abstract
INTRODUCTION: In 2013, the National Tuberculosis (TB) Program of Armenia introduced GeneXpert MTB/RIF (Xpert) assay to address World Health Organization (WHO) target of 80% (2020) of notified new and relapse TB cases to be tested with WHO recommended rapid diagnostic methods. This study aimed to assess the change in laboratory diagnostic profile of Mycobacterium tuberculosis after introduction of the Xpert assay from 2013 to 2017. METHODOLOGY: Retrospective cohort analysis of all presumptive TB patients' records retrieved from the National Reference Laboratory database was performed. RESULTS: This study showed increased trend of Xpert coverage for suspected TB cases from 25% in 2013 to 86% in 2017 which is in line with WHO TB global strategy's target of 80% in 2020. In 4.7% cases, Xpert tested positive while microscopy showed negative results. There was also an improved detection of Rifampicin resistance with increased concordance from 99.1% to 99.4% and decreased discordance from 6.7% to 1.4% between culture and Xpert results. CONCLUSION: Armenia has achieved the 2020 target; in terms of utilizing the GeneXpert it is on track to achieve the End TB strategy target of 100% by 2025. The next step of this research will be assessment of the impact of GeneXpert and other TB tests utilization on the treatment outcomes in Armenia. Copyright (c) 2019 Eduard Yervand Kabasakalyan, Karapet Davtyan, Viktorya Cholakyans, Alvard Mirzoyan, Karine Kentenyants, Diana Petrosyan, Armen Hayrapetyan, Himanshu A. Gupte.
INTRODUCTION: In 2013, the National Tuberculosis (TB) Program of Armenia introduced GeneXpert MTB/RIF (Xpert) assay to address World Health Organization (WHO) target of 80% (2020) of notified new and relapse TB cases to be tested with WHO recommended rapid diagnostic methods. This study aimed to assess the change in laboratory diagnostic profile of Mycobacterium tuberculosis after introduction of the Xpert assay from 2013 to 2017. METHODOLOGY: Retrospective cohort analysis of all presumptive TBpatients' records retrieved from the National Reference Laboratory database was performed. RESULTS: This study showed increased trend of Xpert coverage for suspected TB cases from 25% in 2013 to 86% in 2017 which is in line with WHO TB global strategy's target of 80% in 2020. In 4.7% cases, Xpert tested positive while microscopy showed negative results. There was also an improved detection of Rifampicin resistance with increased concordance from 99.1% to 99.4% and decreased discordance from 6.7% to 1.4% between culture and Xpert results. CONCLUSION: Armenia has achieved the 2020 target; in terms of utilizing the GeneXpert it is on track to achieve the End TB strategy target of 100% by 2025. The next step of this research will be assessment of the impact of GeneXpert and other TB tests utilization on the treatment outcomes in Armenia. Copyright (c) 2019 Eduard Yervand Kabasakalyan, Karapet Davtyan, Viktorya Cholakyans, Alvard Mirzoyan, Karine Kentenyants, Diana Petrosyan, Armen Hayrapetyan, Himanshu A. Gupte.
Authors: F Bahraminia; M Zangiabadian; M J Nasiri; M Fattahi; M Goudarzi; R Ranjbar; A A Imani Fooladi Journal: New Microbes New Infect Date: 2021-06-23