Li Tian1, Lin Yang2, Wenjing Zheng3, Yinqing Hu4, Peikun Ding2, Zheng Wang2, Duo Zheng1, Li Fu1, Bin Chen5, Tian Xiao1, Yuejun Wang1, Feng Chen6, Jun Liu4, Kaiping Gao1, Sipeng Shen7, Rihong Zhai1. 1. School of Public Health, Guangdong Key Laboratory for Genome Stability & Disease Prevention, Carson Cancer Center, Shenzhen University Health Science Center, Shenzhen 518060, PR China. 2. Department of Thoracic Surgery, Shenzhen People's Hospital, Shenzhen 518020, PR China. 3. Department of Hepatobiliary Surgery, Shenzhen University General Hospital, Shenzhen 518060, PR China. 4. Department of Digestive Endoscopy, The First Affiliated Hospital of Shenzhen University, Shenzhen 518023, PR China. 5. Department of Thoracic Surgery, The First Affiliated Hospital of Shenzhen University, Shenzhen 518023, PR China. 6. Department of Biostatistics, Nanjing Medical University, Nanjing 211166, PR China. 7. Department of Environmental Health, Harvard TH Chan School of Public Health, Boston, MA 02115, USA.
Abstract
Aim: To explore the roles of exosomal long noncoding RNAs (lncRNAs) in early-stage esophageal squamous cell carcinoma (ESCC) and benign esophagitis. Materials & methods: Exosomal lncRNAs were analyzed using RNA-seq and validated by quantitative real-time PCR, loss-of-function, co-culture and RNA pulldown assays. Results: Exosomal lncRNAs displayed tighter tissue-specificity, higher expression level and lower splicing efficiency than that of mRNAs. A total of 152 exosomal lncRNAs were differentially expressed between ESCC and controls. A total of 124 exosomal lncRNAs were dysregulated between ESCC and esophagitis. Knockdown of 13 ESCC-associated lncRNAs modified proliferation, migration, and apoptosis of ESCC cells. A novel lncRNA RP5-1092A11.2 was highly expressed in ESCC-derived exosomes, ESCC cells and tumor tissues. Exosomes released from RP5-1092A11.2-knockdown cells inhibited ESCC cell proliferation. Conclusion: Dysregulated exosomal lncRNAs were functionally associated with different disease status in esophagus.
Aim: To explore the roles of exosomal long noncoding RNAs (lncRNAs) in early-stage esophageal squamous cell carcinoma (ESCC) and benign esophagitis. Materials & methods: Exosomal lncRNAs were analyzed using RNA-seq and validated by quantitative real-time PCR, loss-of-function, co-culture and RNA pulldown assays. Results: Exosomal lncRNAs displayed tighter tissue-specificity, higher expression level and lower splicing efficiency than that of mRNAs. A total of 152 exosomal lncRNAs were differentially expressed between ESCC and controls. A total of 124 exosomal lncRNAs were dysregulated between ESCC and esophagitis. Knockdown of 13 ESCC-associated lncRNAs modified proliferation, migration, and apoptosis of ESCC cells. A novel lncRNA RP5-1092A11.2 was highly expressed in ESCC-derived exosomes, ESCC cells and tumor tissues. Exosomes released from RP5-1092A11.2-knockdown cells inhibited ESCC cell proliferation. Conclusion: Dysregulated exosomal lncRNAs were functionally associated with different disease status in esophagus.