Uyen P N Dao1,2, Quan D Nguyen3,2, Trang T Nguyen4,5. 1. School of Biotechnology, International University, Block 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam. 2. Vietnam National University, Ho Chi Minh City, Vietnam. 3. Faculty of Chemical Engineering, HCMC University of Technology, 268 Ly Thuong Kiet, District 10, Ho Chi Minh City, Vietnam. 4. School of Biotechnology, International University, Block 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam. nttrang@hcmiu.edu.vn. 5. Vietnam National University, Ho Chi Minh City, Vietnam. nttrang@hcmiu.edu.vn.
Abstract
PURPOSE: The purpose of this study was to inspect the interactions between an anti-breast cancer, TAM, with model of lipid membranes composed of either zwitterionic DPPC LUVs or anionic DPPG LUVs and how they depend on ionic strength and cholesterol. METHODS: The Kp of TAM into DPPC and DPPG LUVs were determined at three different NaCl concentrations by second derivative UV-Vis spectrophotometry. The effect of cholesterol incorporated into these LUVs on TAM's Kp was also assessed. The ATR-FTIR measurements were carried out to verify structural changes within the acyl chain and head group regions of the liposomes upon TAM partitioning. RESULTS: Increasing salt concentration produced negligible impact on the partitioning of TAM into DPPC bilayer as its Kp remained unaffected whilst induced outstanding reduction of TAM's Kp into DPPG liposomes. Furthermore, TAM was found to disorder the lipids' acyl chains, which could result in an increase in the membrane fluidity, a necessary piece of information to refer to when prescribing TAM dosage for administration. Additionally, cholesterol showed astoundingly opposite contribution to the partitioning of TAM into the LUVs, as its Kp value reduced in DPPC/Chol bilayer yet increased in DPPG/Chol liposomes. CONCLUSION: Ionic strength and cholesterol play a noteworthy role in regulation of TAM partitioning into lipid membranes as they could obstruct or promote such action.
PURPOSE: The purpose of this study was to inspect the interactions between an anti-breast cancer, TAM, with model of lipid membranes composed of either zwitterionic DPPC LUVs or anionic DPPG LUVs and how they depend on ionic strength and cholesterol. METHODS: The Kp of TAM into DPPC and DPPG LUVs were determined at three different NaCl concentrations by second derivative UV-Vis spectrophotometry. The effect of cholesterol incorporated into these LUVs on TAM's Kp was also assessed. The ATR-FTIR measurements were carried out to verify structural changes within the acyl chain and head group regions of the liposomes upon TAM partitioning. RESULTS: Increasing salt concentration produced negligible impact on the partitioning of TAM into DPPC bilayer as its Kp remained unaffected whilst induced outstanding reduction of TAM's Kp into DPPG liposomes. Furthermore, TAM was found to disorder the lipids' acyl chains, which could result in an increase in the membrane fluidity, a necessary piece of information to refer to when prescribing TAM dosage for administration. Additionally, cholesterol showed astoundingly opposite contribution to the partitioning of TAM into the LUVs, as its Kp value reduced in DPPC/Chol bilayer yet increased in DPPG/Chol liposomes. CONCLUSION: Ionic strength and cholesterol play a noteworthy role in regulation of TAM partitioning into lipid membranes as they could obstruct or promote such action.
Authors: Hayet Bensikaddour; Karim Snoussi; Laurence Lins; Françoise Van Bambeke; Paul M Tulkens; Robert Brasseur; Erik Goormaghtigh; Marie-Paule Mingeot-Leclercq Journal: Biochim Biophys Acta Date: 2008-09-06
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Authors: Amirhossein Sahebkar; Maria-Corina Serban; Peter Penson; Camelia Gurban; Sorin Ursoniu; Peter P Toth; Steven R Jones; Giuseppe Lippi; Kazuhiko Kotani; Karam Kostner; Manfredi Rizzo; Jacek Rysz; Maciej Banach Journal: Drugs Date: 2017-07 Impact factor: 9.546