Zihao Pan1, Wenchang Gan2,3, Caiqian Liang4, Yuanxun Xiao2,3, Yu Zhang2,3, Weisheng Yang2,3, Zehui Hou2,3, Shuang Chen2,3, Bing Zeng5, Yingru Li2,3. 1. Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China. 2. Department of Gastroenterology, Hernia and Abdominal Wall Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. 3. Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. 4. Department of Emergency, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China. 5. Department of General Surgery, The Sixth Affiliated Hospital of Guangzhou Medical University, Qing Yuan People's Hospital, Qingyuan 511518, China.
Abstract
BACKGROUND: Colon adenocarcinoma (CA) is the most common one with poor survival in colon cancer. This study aims to investigate the effect of miR-1245a on the process of CA cells and its target gene BRCA2. METHODS: The expression of CA tissues and cells were evaluated by q RT-PCR. Then we explore the association between expression of miR-1245a and prognosis in the CA patients from the TCGA database. CCK8 assays, colony formation assays were performed to explore the effect of miR-1245a in CA cell proliferation. The invasion ability of CA cells was evaluated by Transwell assays. Western blot was performed to assess the BRCA2 expression. Luciferase reporter assay was employed to scrutinize the relationship between miR-1245a and BRCA2. Finally, rescue experiments were performed through BRCA2 downregulation and miR-1245a inhibitors by using colony formation assay and Transwell invasion assay. RESULTS: miR-1245a is upregulated in CA cells and tissues. Additionally, the high expression of miR-1245a was related to poor survival. CCK8 assays, colony formation assays and Transwell assays showed that miR-1245a promotes the proliferation and invasion of CA cells. The luciferase reporter assay indicated that miR-1245a targeted BRCA2 and inhibited its expression. The rescue experiment further showed that miR-1245a could restore the effect of BRCA2 on CA. CONCLUSIONS: miR-1245a promotes the proliferation and invasion of CA by targeting BRCA2.Our results suggested that miR-1245a could be a potential biomarker for CA progression. 2019 Annals of Translational Medicine. All rights reserved.
BACKGROUND: Colon adenocarcinoma (CA) is the most common one with poor survival in colon cancer. This study aims to investigate the effect of miR-1245a on the process of CA cells and its target gene BRCA2. METHODS: The expression of CA tissues and cells were evaluated by q RT-PCR. Then we explore the association between expression of miR-1245a and prognosis in the CA patients from the TCGA database. CCK8 assays, colony formation assays were performed to explore the effect of miR-1245a in CA cell proliferation. The invasion ability of CA cells was evaluated by Transwell assays. Western blot was performed to assess the BRCA2 expression. Luciferase reporter assay was employed to scrutinize the relationship between miR-1245a and BRCA2. Finally, rescue experiments were performed through BRCA2 downregulation and miR-1245a inhibitors by using colony formation assay and Transwell invasion assay. RESULTS: miR-1245a is upregulated in CA cells and tissues. Additionally, the high expression of miR-1245a was related to poor survival. CCK8 assays, colony formation assays and Transwell assays showed that miR-1245a promotes the proliferation and invasion of CA cells. The luciferase reporter assay indicated that miR-1245a targeted BRCA2 and inhibited its expression. The rescue experiment further showed that miR-1245a could restore the effect of BRCA2 on CA. CONCLUSIONS: miR-1245a promotes the proliferation and invasion of CA by targeting BRCA2.Our results suggested that miR-1245a could be a potential biomarker for CA progression. 2019 Annals of Translational Medicine. All rights reserved.
Authors: Juan Zhang; Jie Sun; Jiuan Chen; Lu Yao; Tao Ouyang; Jinfeng Li; Tianfeng Wang; Zhaoqing Fan; Tie Fan; Benyao Lin; Yuntao Xie Journal: Breast Cancer Res Treat Date: 2016-07-08 Impact factor: 4.872
Authors: Bao Chai; Yarong Guo; Xiangli Cui; Jinchun Liu; Yuhong Suo; Zhangfeng Dou; Ning Li Journal: Am J Transl Res Date: 2019-07-15 Impact factor: 4.060
Authors: A A Arbini; F Guerra; M Greco; E Marra; L Gandee; G Xiao; Y Lotan; G Gasparre; J-T Hsieh; L Moro Journal: Oncogenesis Date: 2013-12-16 Impact factor: 7.485