Christina Tsigalou1, Theocharis G Konstantinidis1, Dimitrios Cassimos2, Alexandros Karvelas3, Anastasia Grapsa3, Aggelos Tsalkidis2, Maria Panopoulou1, Athanasios Tsakris4. 1. MD, PhD Laboratory of Microbiology, School of Medicine, Democritus University of Thrace, Dragana 68100, Alexandroupolis, Greece. 2. MD, PhD Department of Pediatrics, University General Hospital of Alexandroupolis, Democritus University of Thrace, Dragana 68100, Alexandroupolis, Greece. 3. MD Laboratory of Microbiology, School of Medicine, Democritus University of Thrace, Dragana 68100, Alexandroupolis, Greece. 4. MD, PhD Laboratory of Microbiology, Medical School, National and Kapodistrian University of Athens, 75 Mikris Asias, Goudi 115 27, Athens, Greece.
Abstract
INTRODUCTION: Helicobacter pylori infection is a well-established etiological factor for a variety of diseases such as peptic ulcer and gastric cancer. On the other hand, there is ongoing research suggesting that H. pylori might have a beneficial effect through a pivotal influence in the immunological response especially in asthma. The aim of the current case-control study was to evaluate the prevalence of H. pylori infection in asthmatic children. METHODS: Twenty-seven children with exacerbation of persistent asthma, aged 8.6±4.5 years (18 males, 9 females) and 54 age-sex-matched non-asthmatic controls were enrolled. Clinical examination and laboratory investigations were performed. Detection of H. pylori antigen (HpSA) in stool samples was performed by a commercial kit (bioNexia® kit, BioMérieux). Serum specific IgG antibodies were detected by a rapid chromatographic immunoassay (DIAsourceImmunoAssays). Serum IgE concentration was determined by electrochemiluminescence (ECL) (Roche Elecsys) and IgE levels ≥ 90 IU/mL were considered significantly elevated. RESULTS: In 3 (11.1%) of the 27 asthmatic children H. pylori infection (based on both detection of HpSA and specific IgG-Abs) was established, whereas as many as 16 of the 54 (29.6%) non-asthmatic ones were found infected (odds ratio 0.1; 95%CI, 0.039-0.305, p=0.026). CONCLUSIONS: Our findings reveal an inverse relationship between H. pylori infection and children's persistent asthma in Greece. GERMS.
INTRODUCTION: Helicobacter pylori infection is a well-established etiological factor for a variety of diseases such as peptic ulcer and gastric cancer. On the other hand, there is ongoing research suggesting that H. pylori might have a beneficial effect through a pivotal influence in the immunological response especially in asthma. The aim of the current case-control study was to evaluate the prevalence of H. pylori infection in asthmatic children. METHODS: Twenty-seven children with exacerbation of persistent asthma, aged 8.6±4.5 years (18 males, 9 females) and 54 age-sex-matched non-asthmatic controls were enrolled. Clinical examination and laboratory investigations were performed. Detection of H. pylori antigen (HpSA) in stool samples was performed by a commercial kit (bioNexia® kit, BioMérieux). Serum specific IgG antibodies were detected by a rapid chromatographic immunoassay (DIAsourceImmunoAssays). Serum IgE concentration was determined by electrochemiluminescence (ECL) (Roche Elecsys) and IgE levels ≥ 90 IU/mL were considered significantly elevated. RESULTS: In 3 (11.1%) of the 27 asthmatic children H. pylori infection (based on both detection of HpSA and specific IgG-Abs) was established, whereas as many as 16 of the 54 (29.6%) non-asthmatic ones were found infected (odds ratio 0.1; 95%CI, 0.039-0.305, p=0.026). CONCLUSIONS: Our findings reveal an inverse relationship between H. pylori infection and children's persistent asthma in Greece. GERMS.
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