Ivan J Fuss1. 1. Mucosal Immunity Section, Laboratory of Clinical Immunology and Microbiology, NIAID, National Institutes of Health, Bethesda, Maryland.
Abstract
This article describes a procedure for isolating T cell subpopulations using various methods including indirect panning and immunopanning by microarray. In these methods, cells are selected by their capacity to bind to antibody-coated plates (or slides) on the basis of particular cell-surface markers. Such methods can be superior to the antibody/complement lysis method (Alternate Protocol), as they can select additional cell population for analysis.
This article describes a procedure for isolating T cell subpopulations using various methods including indirect panning and immunopanning by microarray. In these methods, cells are selected by their capacity to bind to antibody-coated plates (or slides) on the basis of particular cell-surface markers. Such methods can be superior to the antibody/complement lysis method (Alternate Protocol), as they can select additional cell population for analysis.
Authors: R Padmanabhan; C D Corsico; T H Howard; W Holter; C M Fordis; M Willingham; B H Howard Journal: Anal Biochem Date: 1988-05-01 Impact factor: 3.365
Authors: Alina N Khvastunova; Sofya A Kuznetsova; Liubov S Al-Radi; Alexandra V Vylegzhanina; Anna O Zakirova; Olga S Fedyanina; Alexander V Filatov; Ivan A Vorobjev; Fazly Ataullakhanov Journal: Sci Rep Date: 2015-07-27 Impact factor: 4.379