Hepatoprotective Effects of Polysaccharides Isolated from Phellinus gilvus Against Carbon Tetrachloride-induced Liver Injury in Rats.

Phellinus gilvus (PG) is a widely used mushroom for health promotion. We studied the hepatoprotective effect of the polysaccharide aqueous extract of PG (PGP) against carbon tetrachloride (CCl4)-induced liver injury in rats. Sprague Dawley rats were divided into 5 groups: Normal control, CCl4 control, PGP 50, 100, and 200 mg/kg + CCl4. The levels of serum biochemical parameters, liver lipid peroxide and antioxidant enzymes, and histological appearances were evaluated. The CCl4-induced increments of alanine aminotransferase, asparate aminotransferase, and alkaline phosphatase levels in serum were significantly decreased by PGP-pretreatments. The PGP dose-dependently decreased hepatic malondialdehyde formations in CCl4-treatment groups. Hepatic antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) were elevated by PGP in CCl4-treatment groups. Histopathological evaluation of liver showed that the loss of hepatocytes, fatty changes, swelling and extensive necrosis of hepatocytes in centrilobular regions of the CCl4-treated rats were ameliorated by PGP pretreatment. The PGP has hepatoprotective and antioxidative effects in CCl4-induced liver injury of rat. © Korean Society of Toxicology 2009.