Liang Pan1, Chong Sun2, Kang Zhou3, Matteo Figini4, Bin Wang4, Junjie Shangguan4, Su Hu5, Jia Yang4, Wei Xing6, Jian Wang7, Yury Velichko4, Vahid Yaghmai4, Zhuoli Zhang8. 1. Department of Radiology, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China; Department of Radiology, Feinberg School of Medicine, Northwestern University, 737 N. Michigan Ave, 16th Floor, Chicago, IL 60611. 2. Department of Radiology, Feinberg School of Medicine, Northwestern University, 737 N. Michigan Ave, 16th Floor, Chicago, IL 60611; Department of Orthopedics, Affiliated Hospital of Qingdao University, Qingdao, Shandong, China. 3. Department of Radiology, Feinberg School of Medicine, Northwestern University, 737 N. Michigan Ave, 16th Floor, Chicago, IL 60611; Department of Radiology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China. 4. Department of Radiology, Feinberg School of Medicine, Northwestern University, 737 N. Michigan Ave, 16th Floor, Chicago, IL 60611. 5. Department of Radiology, Feinberg School of Medicine, Northwestern University, 737 N. Michigan Ave, 16th Floor, Chicago, IL 60611; Department of Radiology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. 6. Department of Radiology, Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China. 7. Department of Radiology, Feinberg School of Medicine, Northwestern University, 737 N. Michigan Ave, 16th Floor, Chicago, IL 60611; Department of Radiology, Southwest Hospital, Third Military Medical University, Chongqing, China. 8. Department of Radiology, Feinberg School of Medicine, Northwestern University, 737 N. Michigan Ave, 16th Floor, Chicago, IL 60611. Electronic address: zhuoli-zhang@northwestern.edu.
Abstract
RATIONALE AND OBJECTIVES: To investigate whether transcatheter intraarterial perfusion (TRIP) magnetic resonance imaging (MRI) can differentiate reversible electroporation (RE) zones from irreversible electroporation (IRE) zones immediately after IRE procedure in the rabbit liver. MATERIALS AND METHODS: All studies were approved by the institutional animal care and use committee and performed in accordance with institutional guidelines. A total of 13 healthy New Zealand White rabbits were used. After selective catheterization of the hepatic artery under X-ray fluoroscopy, we acquired TRIP-MRI at 20 minutes post-IRE using 3 mL of 5% intraarterial gadopentetate dimeglumine. Semi-quantitative (peak enhancement, PE; time to peak, TTP; wash-in slope, WIS; areas under the time-intensity curve, AUT, over 30, 60, 90, 120, 150, and 180 seconds after the initiation of enhancement) and quantitative (Ktrans, ve, and vp) TRIP-MRI parameters were calculated. The relationships between TRIP-MRI parameters and histological measurements and the differential ability of TRIP-MRI parameters was assessed. RESULTS: PE, AUT60, AUT90, AUT120, AUT150, AUT180, Ktrans, and ve were significantly higher in RE zones than in IRE zones (all P < 0.05), and AUC for these parameters ranged from 0.91(95% CI, 0.80, 1.00) to 0.99 (95% CI, 0.98, 1.00). There was no significant difference in AUC between any two parameters (Z, 0-1.47; P, 0.14-1.00). Hepatocyte apoptosis strongly correlated with PE, AUT60, AUT90, AUT120, AUT150, AUT180, Ktrans, and vp (the absolute value r, 0.6-0.7, all P < 0.0001). CONCLUSION: AUT150 or AUT180 could be a potential imaging biomarker to differentiate RE from IRE zones, and TRIP-MRI permits to differentiate RE from IRE zones immediately after IRE procedure in the rabbit liver. Published by Elsevier Inc.
RATIONALE AND OBJECTIVES: To investigate whether transcatheter intraarterial perfusion (TRIP) magnetic resonance imaging (MRI) can differentiate reversible electroporation (RE) zones from irreversible electroporation (IRE) zones immediately after IRE procedure in the rabbit liver. MATERIALS AND METHODS: All studies were approved by the institutional animal care and use committee and performed in accordance with institutional guidelines. A total of 13 healthy New Zealand White rabbits were used. After selective catheterization of the hepatic artery under X-ray fluoroscopy, we acquired TRIP-MRI at 20 minutes post-IRE using 3 mL of 5% intraarterial gadopentetate dimeglumine. Semi-quantitative (peak enhancement, PE; time to peak, TTP; wash-in slope, WIS; areas under the time-intensity curve, AUT, over 30, 60, 90, 120, 150, and 180 seconds after the initiation of enhancement) and quantitative (Ktrans, ve, and vp) TRIP-MRI parameters were calculated. The relationships between TRIP-MRI parameters and histological measurements and the differential ability of TRIP-MRI parameters was assessed. RESULTS: PE, AUT60, AUT90, AUT120, AUT150, AUT180, Ktrans, and ve were significantly higher in RE zones than in IRE zones (all P < 0.05), and AUC for these parameters ranged from 0.91(95% CI, 0.80, 1.00) to 0.99 (95% CI, 0.98, 1.00). There was no significant difference in AUC between any two parameters (Z, 0-1.47; P, 0.14-1.00). Hepatocyte apoptosis strongly correlated with PE, AUT60, AUT90, AUT120, AUT150, AUT180, Ktrans, and vp (the absolute value r, 0.6-0.7, all P < 0.0001). CONCLUSION: AUT150 or AUT180 could be a potential imaging biomarker to differentiate RE from IRE zones, and TRIP-MRI permits to differentiate RE from IRE zones immediately after IRE procedure in the rabbit liver. Published by Elsevier Inc.
Authors: Anna J Shangguan; Kang Zhou; Jia Yang; Aydin Eresen; Bin Wang; Chong Sun; Liang Pan; Su Hu; Ali T Khan; Samdeep K Mouli; Vahid Yaghmai; Zhuoli Zhang Journal: Clin Exp Gastroenterol Date: 2020-11-06