Literature DB >> 3203157

A novel 190 kDa calmodulin-binding protein associated with brain actomyosin.

R E Larson1, D E Pitta, J A Ferro.   

Abstract

We present evidence for a novel calmodulin-binding protein associated with Ca2+-sensitive, non-muscle actomyosin from rabbit brain. Gel filtration chromatography of brain actomyosin and SDS-PAGE analysis of resulting fractions revealed the presence of a stable complex of a 190 kDa polypeptide and calmodulin. Furthermore, brain actomyosin was retained on a column of conjugated calmodulin-Sepharose 4B and eluted by 6 M urea in the presence of Ca2+. Further elution with 6M urea plus EGTA released the 190 kDa polypeptide from the column. These results show that the 190 kDa polypeptide forms a tight complex with calmodulin and that this complex associates with brain actomyosin in a Ca2+-independent manner.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3203157

Source DB:  PubMed          Journal:  Braz J Med Biol Res        ISSN： 0100-879X            Impact factor:   2.590


  7 in total

1.  The kinetic mechanism of myosin V.

Authors:  E M De La Cruz; A L Wells; S S Rosenfeld; E M Ostap; H L Sweeney
Journal:  Proc Natl Acad Sci U S A       Date:  1999-11-23       Impact factor: 11.205

2.  Primary structure and cellular localization of chicken brain myosin-V (p190), an unconventional myosin with calmodulin light chains.

Authors:  E M Espreafico; R E Cheney; M Matteoli; A A Nascimento; P V De Camilli; R E Larson; M S Mooseker
Journal:  J Cell Biol       Date:  1992-12       Impact factor: 10.539

3.  Regulation of melanosome movement in the cell cycle by reversible association with myosin V.

Authors:  S L Rogers; R L Karcher; J T Roland; A A Minin; W Steffen; V I Gelfand
Journal:  J Cell Biol       Date:  1999-09-20       Impact factor: 10.539

4.  Calmodulin concentrates at regions of cell growth in Saccharomyces cerevisiae.

Authors:  S E Brockerhoff; T N Davis
Journal:  J Cell Biol       Date:  1992-08       Impact factor: 10.539

5.  Biochemical and immunological characterization of p190-calmodulin complex from vertebrate brain: a novel calmodulin-binding myosin.

Authors:  F S Espindola; E M Espreafico; M V Coelho; A R Martins; F R Costa; M S Mooseker; R E Larson
Journal:  J Cell Biol       Date:  1992-07       Impact factor: 10.539

6.  Differential localization of Acanthamoeba myosin I isoforms.

Authors:  I C Baines; H Brzeska; E D Korn
Journal:  J Cell Biol       Date:  1992-12       Impact factor: 10.539

7.  The unconventional myosin, Myo2p, is a calmodulin target at sites of cell growth in Saccharomyces cerevisiae.

Authors:  S E Brockerhoff; R C Stevens; T N Davis
Journal:  J Cell Biol       Date:  1994-02       Impact factor: 10.539
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.