Yang Zhang1, Hai Guo2, Li Ma3, Jinyu Zhu1, Anyun Guo1, Yong He1. 1. Department of Joint Trauma, General Hospital of Shenzhen University, Shenzhen Guangdong, 518055, P.R.China. 2. Department of Anesthesiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi Xinjiang, 830054, P.R.China. 3. Department of Emergency, General Hospital of Xinjiang Military Region, Urumqi Xinjiang, 830000, P.R.China.
Abstract
OBJECTIVE: To investigate the regulatory effect of long chain non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) adsorbing microRNA-124 (miR-124) on osteogenic differentiation of mesenchymal stem cells (MSCs). METHODS: C3H10T1/2 cells derived from mouse embryos were cultured in vitro, then randomly divided into control group (group A), lncRNA MALAT1 no-load plasmid group (group B), lncRNA MALAT1 overexpression plasmid group (group C), lncRNA MALAT1 small interfering RNA (siRNA) group (group D), and lncRNA MALAT1 siRNA negative control group (group E). The cells were transfected into plasmids and siRNA, then induced to differentiate into osteoblasts. Alkaline phosphatase (ALP) and alizarin red staining were used to detect the osteogenic differentiation of cells in each group, real-time fluorescence quantitative (qRT-PCR) analysis was used to detect the expressions of lncRNA MALAT, miR-124, and osteogenesis-related genes such as Runt-related transcription factor 2 (Runx2), osteopontin (OPN), and osteocalcin (OCN) in each group. Double luciferase reporter gene was used to detect the targeting regulation of lncRNA MALAT1 to miR-124. RESULTS: The relative contents of ALP positive cells, mineralized nodule, and the relative mRNA expressions of lncRNA MALAT1, Runx2, OPN, and OCN in group C were significantly higher than those in other groups ( P<0.05), while in group D significantly lower than in other groups ( P<0.05); the relative expression of miR-124 in group C was significantly lower than that in other groups( P<0.05), while in group D significantly higher than in other groups ( P<0.05). There was no significant difference in these indexes between groups A, B, and E ( P>0.05). The results of double luciferase reporter gene assay showed that lncRNA MALAT1 targeting down-regulated the expression of miR-124. CONCLUSION: LncRNA MALAT1 can targeting down-regulate the expression of miR-124 and promote the osteogenic differentiation of MSCs.
OBJECTIVE: To investigate the regulatory effect of long chain non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) adsorbing microRNA-124 (miR-124) on osteogenic differentiation of mesenchymal stem cells (MSCs). METHODS: C3H10T1/2 cells derived from mouse embryos were cultured in vitro, then randomly divided into control group (group A), lncRNA MALAT1 no-load plasmid group (group B), lncRNA MALAT1 overexpression plasmid group (group C), lncRNA MALAT1 small interfering RNA (siRNA) group (group D), and lncRNA MALAT1 siRNA negative control group (group E). The cells were transfected into plasmids and siRNA, then induced to differentiate into osteoblasts. Alkaline phosphatase (ALP) and alizarin red staining were used to detect the osteogenic differentiation of cells in each group, real-time fluorescence quantitative (qRT-PCR) analysis was used to detect the expressions of lncRNA MALAT, miR-124, and osteogenesis-related genes such as Runt-related transcription factor 2 (Runx2), osteopontin (OPN), and osteocalcin (OCN) in each group. Double luciferase reporter gene was used to detect the targeting regulation of lncRNA MALAT1 to miR-124. RESULTS: The relative contents of ALP positive cells, mineralized nodule, and the relative mRNA expressions of lncRNA MALAT1, Runx2, OPN, and OCN in group C were significantly higher than those in other groups ( P<0.05), while in group D significantly lower than in other groups ( P<0.05); the relative expression of miR-124 in group C was significantly lower than that in other groups( P<0.05), while in group D significantly higher than in other groups ( P<0.05). There was no significant difference in these indexes between groups A, B, and E ( P>0.05). The results of double luciferase reporter gene assay showed that lncRNA MALAT1 targeting down-regulated the expression of miR-124. CONCLUSION: LncRNA MALAT1 can targeting down-regulate the expression of miR-124 and promote the osteogenic differentiation of MSCs.
Authors: Huan Jing; Xiaoxia Su; Bo Gao; Yi Shuai; Ji Chen; Zhihong Deng; Li Liao; Yan Jin Journal: Cell Death Dis Date: 2018-02-07 Impact factor: 8.469
Authors: Carmen Lanzillotti; Monica De Mattei; Chiara Mazziotta; Francesca Taraballi; John Charles Rotondo; Mauro Tognon; Fernanda Martini Journal: Front Cell Dev Biol Date: 2021-04-09