Sebastian Blatt1,2, Valentin Burkhardt3, Peer W Kämmerer3, Andreas M Pabst4, Keyvan Sagheb3, Martin Heller5, Bilal Al-Nawas3, Eik Schiegnitz3. 1. Department of Oral- and Maxillofacial Surgery, University Medical Center, Augustusplatz 2, 55131, Mainz, Germany. sebastian.blatt@unimedizin-mainz.de. 2. Platform for Biomaterial Research, Biomatics Group, University Medical Center, Langenbeckstrasse 1, 55131, Mainz, Germany. sebastian.blatt@unimedizin-mainz.de. 3. Department of Oral- and Maxillofacial Surgery, University Medical Center, Augustusplatz 2, 55131, Mainz, Germany. 4. Department of Oral- and Maxillofacial Surgery, Federal Armed Forces Hospital, Rübenacher Str. 170, 56072, Koblenz, Germany. 5. Department of Gynecology, University Medical Center, Langenbeckstrasse 1, 55131, Mainz, Germany.
Abstract
OBJECTIVES: Porcine-derived collagen matrices (CM) can be used for oral tissue regeneration, but sufficient revascularization is crucial. The aim of this study was to analyze the influence of platelet-rich fibrin (PRF) on angiogenesis of different CM in vitro and in vivo. MATERIALS AND METHODS: Three different CM (mucoderm®, jason®, collprotect®) were combined with PRF in a plotting process. Growth factor release (VEGF, TGF-β) was measured in vitro via ELISA quantification after 1,4 and 7 days in comparison to PRF alone. In ovo yolk sac (YSM) and chorion allantois membrane (CAM) model, angiogenic potential were analyzed in vivo with light- and intravital fluorescence microscopy after 24 h, then verified with immunohistochemical staining for CD105 and αSMA. RESULTS: Highest growth factor release was seen after 24 h for all three activated membranes in comparison to the native CM (VEGF 24 h: each p < 0.05; TGF-β: each p < 0.001) and the PRF (no significant difference). All activated membranes revealed a significantly increased angiogenic potential in vivo after 24 h (vessels per mm2: each p < 0.05; branching points per mm2: each p < 0.01; vessel density: each p < 0.05) and with immunohistochemical staining for CD105 (each p < 0.01) and αSMA (each p < 0.05). CONCLUSIONS: PRF improved the angiogenesis of CM in vitro and in vivo. CLINICAL RELEVANCE: Bio-functionalization of CM with PRF could easily implemented in the clinical pathway and may lead to advanced soft tissue healing.
OBJECTIVES: Porcine-derived collagen matrices (CM) can be used for oral tissue regeneration, but sufficient revascularization is crucial. The aim of this study was to analyze the influence of platelet-rich fibrin (PRF) on angiogenesis of different CM in vitro and in vivo. MATERIALS AND METHODS: Three different CM (mucoderm®, jason®, collprotect®) were combined with PRF in a plotting process. Growth factor release (VEGF, TGF-β) was measured in vitro via ELISA quantification after 1,4 and 7 days in comparison to PRF alone. In ovo yolk sac (YSM) and chorion allantois membrane (CAM) model, angiogenic potential were analyzed in vivo with light- and intravital fluorescence microscopy after 24 h, then verified with immunohistochemical staining for CD105 and αSMA. RESULTS: Highest growth factor release was seen after 24 h for all three activated membranes in comparison to the native CM (VEGF 24 h: each p < 0.05; TGF-β: each p < 0.001) and the PRF (no significant difference). All activated membranes revealed a significantly increased angiogenic potential in vivo after 24 h (vessels per mm2: each p < 0.05; branching points per mm2: each p < 0.01; vessel density: each p < 0.05) and with immunohistochemical staining for CD105 (each p < 0.01) and αSMA (each p < 0.05). CONCLUSIONS: PRF improved the angiogenesis of CM in vitro and in vivo. CLINICAL RELEVANCE: Bio-functionalization of CM with PRF could easily implemented in the clinical pathway and may lead to advanced soft tissue healing.
Authors: Diana Heimes; Nadine Wiesmann; Jonas Eckrich; Juergen Brieger; Stefan Mattyasovszky; Peter Proff; Manuel Weber; James Deschner; Bilal Al-Nawas; Peer W Kämmerer Journal: Int J Mol Sci Date: 2020-10-14 Impact factor: 5.923
Authors: Solomiya Kyyak; Sebastian Blatt; Eik Schiegnitz; Diana Heimes; Henning Staedt; Daniel G E Thiem; Keyvan Sagheb; Bilal Al-Nawas; Peer W Kämmerer Journal: Front Bioeng Biotechnol Date: 2021-02-17
Authors: Javier Aragoneses; Ana Suárez; Cinthia Rodríguez; Juan Manuel Aragoneses Journal: Int J Environ Res Public Health Date: 2021-04-07 Impact factor: 3.390
Authors: Sebastian Blatt; Daniel G E Thiem; Solomiya Kyyak; Andreas Pabst; Bilal Al-Nawas; Peer W Kämmerer Journal: Front Bioeng Biotechnol Date: 2021-03-16
Authors: Henning Staedt; Michael Dau; Eik Schiegnitz; Daniel G E Thiem; Olga Tagadiuc; Victor Palarie; Peter Ottl; Bilal Al-Nawas; Peer W Kämmerer Journal: Head Face Med Date: 2020-12-07 Impact factor: 2.151
Authors: Sebastian Blatt; Maximilian Krüger; Peer W Kämmerer; Daniel G E Thiem; Philipp Matheis; Anne-Katrin Eisenbeiß; Jörg Wiltfang; Bilal Al-Nawas; Hendrik Naujokat Journal: J Clin Med Date: 2022-01-28 Impact factor: 4.241