Wengang Yang1, Dezhong Wen1, Shaoxi Chen2, Song Xue1, Jianmin Gu1, Jianggui Dan1, Hui Zheng1. 1. Department of Cardiovascular Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China. 2. Department of Cardiovascular Surgery, Zhe Jiang Hospital, Hangzhou 310030, China.
Abstract
BACKGROUND: To detect the expression of Nesprin-1 in aortic dissection (AD) in patients and to investigate the role of Nesprin-1 in the pathogenesis of AD in a mouse model. METHODS: Blood and tissue specimens from AD patients were collected. The expression of Nesprin-1 in tissues from AD patients and non-AD patients with heart disease was studied by western blotting and quantitative real-time polymerase chain reaction (qRT-PCR). In addition, the expression and distribution of Nesprin-1 in AD and sham mice were compared in an induced AD mouse model, and detected by immunohistochemistry and qRT-PCR. RESULTS: Immunoblotting and qRT-PCR both showed that the expression of Nesprin-1 was significantly higher in AD versus control patients. An animal model of AD was established by continuous injection of Ang II into ApoE-/- mice. The expression of Nesprin-1 in aortic tissue of AD mice was higher than that of sham-operated mice as determined by immunohistochemistry. qRT-PCR showed that Nesprin-1 gene expression in aorta of AD mice was higher than that of sham-operated mice. CONCLUSIONS: An increased expression of Nesprin-1 was associated with AD, and hence Nesprin-1 may be involved in the pathogenesis of ADs. Preliminary findings suggest that Nesprin-1 may be a therapeutic target for the treatment of AD. 2019 Journal of Thoracic Disease. All rights reserved.
BACKGROUND: To detect the expression of Nesprin-1 in aortic dissection (AD) in patients and to investigate the role of Nesprin-1 in the pathogenesis of AD in a mouse model. METHODS: Blood and tissue specimens from AD patients were collected. The expression of Nesprin-1 in tissues from AD patients and non-AD patients with heart disease was studied by western blotting and quantitative real-time polymerase chain reaction (qRT-PCR). In addition, the expression and distribution of Nesprin-1 in AD and sham mice were compared in an induced AD mouse model, and detected by immunohistochemistry and qRT-PCR. RESULTS: Immunoblotting and qRT-PCR both showed that the expression of Nesprin-1 was significantly higher in AD versus control patients. An animal model of AD was established by continuous injection of Ang II into ApoE-/- mice. The expression of Nesprin-1 in aortic tissue of AD mice was higher than that of sham-operated mice as determined by immunohistochemistry. qRT-PCR showed that Nesprin-1 gene expression in aorta of AD mice was higher than that of sham-operated mice. CONCLUSIONS: An increased expression of Nesprin-1 was associated with AD, and hence Nesprin-1 may be involved in the pathogenesis of ADs. Preliminary findings suggest that Nesprin-1 may be a therapeutic target for the treatment of AD. 2019 Journal of Thoracic Disease. All rights reserved.
Authors: Qiuping Zhang; Cassandra D Ragnauth; Jeremy N Skepper; Nathalie F Worth; Derek T Warren; Roland G Roberts; Peter L Weissberg; Juliet A Ellis; Catherine M Shanahan Journal: J Cell Sci Date: 2005-01-25 Impact factor: 5.285
Authors: V C Padmakumar; Sabu Abraham; Stephan Braune; Angelika A Noegel; Budi Tunggal; Iakowos Karakesisoglou; Elena Korenbaum Journal: Exp Cell Res Date: 2004-05-01 Impact factor: 3.905