Literature DB >> 3202951

Molecular cloning of bovine beta-lactoglobulin cDNA.

V N Ivanov1, E S Judinkova, S I Gorodetsky.   

Abstract

A cDNA library from bovine mammary gland mRNA was constructed in pBR322 and screened by hybrid-selected translation and immunoscreening. Several beta-lactoglobulin clones were identified and sequenced. All clones contained cDNA fragments corresponding to the 3' region of beta-lactoglobulin mRNA. The 3' non-translated region of beta-lactoglobulin mRNA consists of 187 nucleotides; the polyadenylation signal AATAAA occurs 17 nucleotides before the poly(A) tail. The amino-acid sequence predicted from the 3' coding region corresponds completely to the previously determined amino-acid sequence of beta-lactoglobulin.

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Year:  1988        PMID: 3202951     DOI: 10.1515/bchm3.1988.369.1.425

Source DB:  PubMed          Journal:  Biol Chem Hoppe Seyler        ISSN: 0177-3593


  2 in total

1.  Cloning and partial nucleotide sequence of the genomic bovine beta-lactoglobulin gene.

Authors:  M C Silva; D W Wong; C A Batt
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

2.  Complete sequence of the bovine beta-lactoglobulin cDNA.

Authors:  L J Alexander; G Hayes; M J Pearse; C W Beattie; A F Stewart; I M Willis; A G Mackinlay
Journal:  Nucleic Acids Res       Date:  1989-08-25       Impact factor: 16.971

  2 in total

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