Literature DB >> 32027730

Correction: An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome.

Yanlong Wei, Pingping Deng, Ali Mohsin, Yan Yang, Huayan Zhou, Meijin Guo, Hongqing Fang.   

Abstract

[This corrects the article DOI: 10.1371/journal.pone.0186891.].

Entities:  

Year:  2020        PMID: 32027730      PMCID: PMC7004325          DOI: 10.1371/journal.pone.0229072

Source DB:  PubMed          Journal:  PLoS One        ISSN: 1932-6203            Impact factor:   3.240


The following information is missing from the Data Availability statement: The plasmids described in this study are available from Addgene: plasmid pBDC, Addgene ID: 135188 plasmid pBDK, Addgene ID: 135190 plasmid pCNA, Addgene ID: 135191 plasmid pSNA, Addgene ID: 135192 plasmid pSNK, Addgene ID: 135193 There is an error in S2 Table. The fourteenth base I-SceI should be “T,” not “A.” The correct S2 Table provided here shows the whole sequence of plasmid pSNA (Addgene ID: 135192).

Sequences of I-SceI, I-CreI, and GC cassette.

(DOCX) Click here for additional data file.
  1 in total

1.  An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome.

Authors:  Yanlong Wei; Pingping Deng; Ali Mohsin; Yan Yang; Huayan Zhou; Meijin Guo; Hongqing Fang
Journal:  PLoS One       Date:  2017-10-24       Impact factor: 3.240

  1 in total

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